Temperature induced changes in the optical properties of skin in vivo

Abstract Knowledge of temperature-induced changes of skin optical properties is required for accurate dosimetry of photothermal treatments. We determined and compared in vivo optical properties of mouse ear skin at different temperatures. The diffuse reflectance, total and diffuse transmittance were...

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Autores principales: Tyler W. Iorizzo, Peter R. Jermain, Elena Salomatina, Alona Muzikansky, Anna N. Yaroslavsky
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Publicado: Nature Portfolio 2021
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spelling oai:doaj.org-article:179822a6c427474cafe15520a85324352021-12-02T14:12:45ZTemperature induced changes in the optical properties of skin in vivo10.1038/s41598-020-80254-92045-2322https://doaj.org/article/179822a6c427474cafe15520a85324352021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80254-9https://doaj.org/toc/2045-2322Abstract Knowledge of temperature-induced changes of skin optical properties is required for accurate dosimetry of photothermal treatments. We determined and compared in vivo optical properties of mouse ear skin at different temperatures. The diffuse reflectance, total and diffuse transmittance were measured in the spectral range from 400 to 1650 nm using an integrating sphere spectrometer at the temperatures of 25 °C, 36 °C and 60 °C. Target temperatures were attained and maintained using an automated heater equipped with a sensor for feed-back and control. Temperature and temperature induced morphological changes of skin were monitored using an infrared thermal camera and reflectance confocal microscopy, respectively. An inverse Monte Carlo technique was utilized to determine absorption, scattering, and anisotropy factors from the measured quantities. Our results indicate significant differences between the optical properties of skin at different temperatures. Absorption and scattering coefficients increased, whereas anisotropy factors decreased with increasing temperature. Changes in absorption coefficients indicate deoxygenation of hemoglobin, and a blue shift of water absorption bands. Confocal imaging confirmed that our observations can be explained by temperature induced protein denaturation and blood coagulation. Monitoring spectral responses of treated tissue may become a valuable tool for accurate dosimetry of light treatments.Tyler W. IorizzoPeter R. JermainElena SalomatinaAlona MuzikanskyAnna N. YaroslavskyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Tyler W. Iorizzo
Peter R. Jermain
Elena Salomatina
Alona Muzikansky
Anna N. Yaroslavsky
Temperature induced changes in the optical properties of skin in vivo
description Abstract Knowledge of temperature-induced changes of skin optical properties is required for accurate dosimetry of photothermal treatments. We determined and compared in vivo optical properties of mouse ear skin at different temperatures. The diffuse reflectance, total and diffuse transmittance were measured in the spectral range from 400 to 1650 nm using an integrating sphere spectrometer at the temperatures of 25 °C, 36 °C and 60 °C. Target temperatures were attained and maintained using an automated heater equipped with a sensor for feed-back and control. Temperature and temperature induced morphological changes of skin were monitored using an infrared thermal camera and reflectance confocal microscopy, respectively. An inverse Monte Carlo technique was utilized to determine absorption, scattering, and anisotropy factors from the measured quantities. Our results indicate significant differences between the optical properties of skin at different temperatures. Absorption and scattering coefficients increased, whereas anisotropy factors decreased with increasing temperature. Changes in absorption coefficients indicate deoxygenation of hemoglobin, and a blue shift of water absorption bands. Confocal imaging confirmed that our observations can be explained by temperature induced protein denaturation and blood coagulation. Monitoring spectral responses of treated tissue may become a valuable tool for accurate dosimetry of light treatments.
format article
author Tyler W. Iorizzo
Peter R. Jermain
Elena Salomatina
Alona Muzikansky
Anna N. Yaroslavsky
author_facet Tyler W. Iorizzo
Peter R. Jermain
Elena Salomatina
Alona Muzikansky
Anna N. Yaroslavsky
author_sort Tyler W. Iorizzo
title Temperature induced changes in the optical properties of skin in vivo
title_short Temperature induced changes in the optical properties of skin in vivo
title_full Temperature induced changes in the optical properties of skin in vivo
title_fullStr Temperature induced changes in the optical properties of skin in vivo
title_full_unstemmed Temperature induced changes in the optical properties of skin in vivo
title_sort temperature induced changes in the optical properties of skin in vivo
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/179822a6c427474cafe15520a8532435
work_keys_str_mv AT tylerwiorizzo temperatureinducedchangesintheopticalpropertiesofskininvivo
AT peterrjermain temperatureinducedchangesintheopticalpropertiesofskininvivo
AT elenasalomatina temperatureinducedchangesintheopticalpropertiesofskininvivo
AT alonamuzikansky temperatureinducedchangesintheopticalpropertiesofskininvivo
AT annanyaroslavsky temperatureinducedchangesintheopticalpropertiesofskininvivo
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