Temperature induced changes in the optical properties of skin in vivo

Abstract Knowledge of temperature-induced changes of skin optical properties is required for accurate dosimetry of photothermal treatments. We determined and compared in vivo optical properties of mouse ear skin at different temperatures. The diffuse reflectance, total and diffuse transmittance were...

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Autores principales:	Tyler W. Iorizzo, Peter R. Jermain, Elena Salomatina, Alona Muzikansky, Anna N. Yaroslavsky
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/179822a6c427474cafe15520a8532435
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spelling	oai:doaj.org-article:179822a6c427474cafe15520a85324352021-12-02T14:12:45ZTemperature induced changes in the optical properties of skin in vivo10.1038/s41598-020-80254-92045-2322https://doaj.org/article/179822a6c427474cafe15520a85324352021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80254-9https://doaj.org/toc/2045-2322Abstract Knowledge of temperature-induced changes of skin optical properties is required for accurate dosimetry of photothermal treatments. We determined and compared in vivo optical properties of mouse ear skin at different temperatures. The diffuse reflectance, total and diffuse transmittance were measured in the spectral range from 400 to 1650 nm using an integrating sphere spectrometer at the temperatures of 25 °C, 36 °C and 60 °C. Target temperatures were attained and maintained using an automated heater equipped with a sensor for feed-back and control. Temperature and temperature induced morphological changes of skin were monitored using an infrared thermal camera and reflectance confocal microscopy, respectively. An inverse Monte Carlo technique was utilized to determine absorption, scattering, and anisotropy factors from the measured quantities. Our results indicate significant differences between the optical properties of skin at different temperatures. Absorption and scattering coefficients increased, whereas anisotropy factors decreased with increasing temperature. Changes in absorption coefficients indicate deoxygenation of hemoglobin, and a blue shift of water absorption bands. Confocal imaging confirmed that our observations can be explained by temperature induced protein denaturation and blood coagulation. Monitoring spectral responses of treated tissue may become a valuable tool for accurate dosimetry of light treatments.Tyler W. IorizzoPeter R. JermainElena SalomatinaAlona MuzikanskyAnna N. YaroslavskyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tyler W. Iorizzo Peter R. Jermain Elena Salomatina Alona Muzikansky Anna N. Yaroslavsky Temperature induced changes in the optical properties of skin in vivo
description	Abstract Knowledge of temperature-induced changes of skin optical properties is required for accurate dosimetry of photothermal treatments. We determined and compared in vivo optical properties of mouse ear skin at different temperatures. The diffuse reflectance, total and diffuse transmittance were measured in the spectral range from 400 to 1650 nm using an integrating sphere spectrometer at the temperatures of 25 °C, 36 °C and 60 °C. Target temperatures were attained and maintained using an automated heater equipped with a sensor for feed-back and control. Temperature and temperature induced morphological changes of skin were monitored using an infrared thermal camera and reflectance confocal microscopy, respectively. An inverse Monte Carlo technique was utilized to determine absorption, scattering, and anisotropy factors from the measured quantities. Our results indicate significant differences between the optical properties of skin at different temperatures. Absorption and scattering coefficients increased, whereas anisotropy factors decreased with increasing temperature. Changes in absorption coefficients indicate deoxygenation of hemoglobin, and a blue shift of water absorption bands. Confocal imaging confirmed that our observations can be explained by temperature induced protein denaturation and blood coagulation. Monitoring spectral responses of treated tissue may become a valuable tool for accurate dosimetry of light treatments.
format	article
author	Tyler W. Iorizzo Peter R. Jermain Elena Salomatina Alona Muzikansky Anna N. Yaroslavsky
author_facet	Tyler W. Iorizzo Peter R. Jermain Elena Salomatina Alona Muzikansky Anna N. Yaroslavsky
author_sort	Tyler W. Iorizzo
title	Temperature induced changes in the optical properties of skin in vivo
title_short	Temperature induced changes in the optical properties of skin in vivo
title_full	Temperature induced changes in the optical properties of skin in vivo
title_fullStr	Temperature induced changes in the optical properties of skin in vivo
title_full_unstemmed	Temperature induced changes in the optical properties of skin in vivo
title_sort	temperature induced changes in the optical properties of skin in vivo
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/179822a6c427474cafe15520a8532435
work_keys_str_mv	AT tylerwiorizzo temperatureinducedchangesintheopticalpropertiesofskininvivo AT peterrjermain temperatureinducedchangesintheopticalpropertiesofskininvivo AT elenasalomatina temperatureinducedchangesintheopticalpropertiesofskininvivo AT alonamuzikansky temperatureinducedchangesintheopticalpropertiesofskininvivo AT annanyaroslavsky temperatureinducedchangesintheopticalpropertiesofskininvivo
_version_	1718391770741473280

Temperature induced changes in the optical properties of skin in vivo

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