DETECTION OF AMP-C BETA-LACTAMASES IN URINARY ISOLATES OF ESCHERICHIA COLI THROUGH VARIOUS PHENOTYPIC METHODS
Objective: To determine the prevalence of Amp C beta lactamases in urinary isolates of Escherichia coli (E. coli), and to evaluate and compare different phenotypic methods for its detection in a cost effective way. Study Design: Descriptive study. Place and Duration of Study: Department of Microb...
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| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Army Medical College Rawalpindi
2018
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/179a6ce951264831a18b50e18befe93c |
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| Sumario: | Objective: To determine the prevalence of Amp C beta lactamases in urinary isolates of Escherichia coli (E. coli),
and to evaluate and compare different phenotypic methods for its detection in a cost effective way.
Study Design: Descriptive study.
Place and Duration of Study: Department of Microbiology, Combined Military Hospital Lahore Pakistan, from
Jan 2016 to Jun 2016.
Material and Methods: Modified Three Dimensional Test (M3DT) taken as Gold standard, Modified Hodge Test
(MHT) (Cefoxitin), Nitrocephin test and three screening strategies for the detection of Amp C Beta-lactamases
were tested on urinary isolates of E. coli collected during a period of 06 months.
Results: Modified Hodge Test, was found to be simple, highly specific and sensitive in detecting these enzyme
producers. Collectively these tests detected 45.07% of E. coli to be Amp C producers.
Conclusion: Each of the three tests can be used as an acceptable phenotypic confirmatory tool when Amp C production
in E. coli is suspected. |
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