DETECTION OF AMP-C BETA-LACTAMASES IN URINARY ISOLATES OF ESCHERICHIA COLI THROUGH VARIOUS PHENOTYPIC METHODS

Objective: To determine the prevalence of Amp C beta lactamases in urinary isolates of Escherichia coli (E. coli), and to evaluate and compare different phenotypic methods for its detection in a cost effective way. Study Design: Descriptive study. Place and Duration of Study: Department of Microb...

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Autores principales:	Qanita Fahim, Ayesha Khalid, Fatima Hameed, Saeed Anwar
Formato:	article
Lenguaje:	EN
Publicado:	Army Medical College Rawalpindi 2018
Materias:	amp c beta-lactamases Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/179a6ce951264831a18b50e18befe93c
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spelling	oai:doaj.org-article:179a6ce951264831a18b50e18befe93c2021-12-02T06:43:36ZDETECTION OF AMP-C BETA-LACTAMASES IN URINARY ISOLATES OF ESCHERICHIA COLI THROUGH VARIOUS PHENOTYPIC METHODS0030-96482411-8842https://doaj.org/article/179a6ce951264831a18b50e18befe93c2018-10-01T00:00:00Zhttps://www.pafmj.org/index.php/PAFMJ/article/view/2308/2003https://doaj.org/toc/0030-9648https://doaj.org/toc/2411-8842Objective: To determine the prevalence of Amp C beta lactamases in urinary isolates of Escherichia coli (E. coli), and to evaluate and compare different phenotypic methods for its detection in a cost effective way. Study Design: Descriptive study. Place and Duration of Study: Department of Microbiology, Combined Military Hospital Lahore Pakistan, from Jan 2016 to Jun 2016. Material and Methods: Modified Three Dimensional Test (M3DT) taken as Gold standard, Modified Hodge Test (MHT) (Cefoxitin), Nitrocephin test and three screening strategies for the detection of Amp C Beta-lactamases were tested on urinary isolates of E. coli collected during a period of 06 months. Results: Modified Hodge Test, was found to be simple, highly specific and sensitive in detecting these enzyme producers. Collectively these tests detected 45.07% of E. coli to be Amp C producers. Conclusion: Each of the three tests can be used as an acceptable phenotypic confirmatory tool when Amp C production in E. coli is suspected.Qanita FahimAyesha KhalidFatima HameedSaeed AnwarArmy Medical College Rawalpindiarticleamp c beta-lactamasesMedicineRMedicine (General)R5-920ENPakistan Armed Forces Medical Journal, Vol 68, Iss 5, Pp 1282-1287 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	amp c beta-lactamases Medicine R Medicine (General) R5-920
spellingShingle	amp c beta-lactamases Medicine R Medicine (General) R5-920 Qanita Fahim Ayesha Khalid Fatima Hameed Saeed Anwar DETECTION OF AMP-C BETA-LACTAMASES IN URINARY ISOLATES OF ESCHERICHIA COLI THROUGH VARIOUS PHENOTYPIC METHODS
description	Objective: To determine the prevalence of Amp C beta lactamases in urinary isolates of Escherichia coli (E. coli), and to evaluate and compare different phenotypic methods for its detection in a cost effective way. Study Design: Descriptive study. Place and Duration of Study: Department of Microbiology, Combined Military Hospital Lahore Pakistan, from Jan 2016 to Jun 2016. Material and Methods: Modified Three Dimensional Test (M3DT) taken as Gold standard, Modified Hodge Test (MHT) (Cefoxitin), Nitrocephin test and three screening strategies for the detection of Amp C Beta-lactamases were tested on urinary isolates of E. coli collected during a period of 06 months. Results: Modified Hodge Test, was found to be simple, highly specific and sensitive in detecting these enzyme producers. Collectively these tests detected 45.07% of E. coli to be Amp C producers. Conclusion: Each of the three tests can be used as an acceptable phenotypic confirmatory tool when Amp C production in E. coli is suspected.
format	article
author	Qanita Fahim Ayesha Khalid Fatima Hameed Saeed Anwar
author_facet	Qanita Fahim Ayesha Khalid Fatima Hameed Saeed Anwar
author_sort	Qanita Fahim
title	DETECTION OF AMP-C BETA-LACTAMASES IN URINARY ISOLATES OF ESCHERICHIA COLI THROUGH VARIOUS PHENOTYPIC METHODS
title_short	DETECTION OF AMP-C BETA-LACTAMASES IN URINARY ISOLATES OF ESCHERICHIA COLI THROUGH VARIOUS PHENOTYPIC METHODS
title_full	DETECTION OF AMP-C BETA-LACTAMASES IN URINARY ISOLATES OF ESCHERICHIA COLI THROUGH VARIOUS PHENOTYPIC METHODS
title_fullStr	DETECTION OF AMP-C BETA-LACTAMASES IN URINARY ISOLATES OF ESCHERICHIA COLI THROUGH VARIOUS PHENOTYPIC METHODS
title_full_unstemmed	DETECTION OF AMP-C BETA-LACTAMASES IN URINARY ISOLATES OF ESCHERICHIA COLI THROUGH VARIOUS PHENOTYPIC METHODS
title_sort	detection of amp-c beta-lactamases in urinary isolates of escherichia coli through various phenotypic methods
publisher	Army Medical College Rawalpindi
publishDate	2018
url	https://doaj.org/article/179a6ce951264831a18b50e18befe93c
work_keys_str_mv	AT qanitafahim detectionofampcbetalactamasesinurinaryisolatesofescherichiacolithroughvariousphenotypicmethods AT ayeshakhalid detectionofampcbetalactamasesinurinaryisolatesofescherichiacolithroughvariousphenotypicmethods AT fatimahameed detectionofampcbetalactamasesinurinaryisolatesofescherichiacolithroughvariousphenotypicmethods AT saeedanwar detectionofampcbetalactamasesinurinaryisolatesofescherichiacolithroughvariousphenotypicmethods
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DETECTION OF AMP-C BETA-LACTAMASES IN URINARY ISOLATES OF ESCHERICHIA COLI THROUGH VARIOUS PHENOTYPIC METHODS

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