A high-throughput pipeline for design and selection of peptides targeting the SARS-Cov-2 Spike protein

Abstract Rapid design, screening, and characterization of biorecognition elements (BREs) is essential for the development of diagnostic tests and antiviral therapeutics needed to combat the spread of viruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). To address this need,...

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Autores principales:	Monica Wolfe, Sean Webb, Yaroslav Chushak, Rachel Krabacher, Yi Liu, Nathan Swami, Svetlana Harbaugh, Jorge Chávez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/179bddfe608341f48fcb672122105c86
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spelling	oai:doaj.org-article:179bddfe608341f48fcb672122105c862021-11-08T10:55:19ZA high-throughput pipeline for design and selection of peptides targeting the SARS-Cov-2 Spike protein10.1038/s41598-021-01225-22045-2322https://doaj.org/article/179bddfe608341f48fcb672122105c862021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01225-2https://doaj.org/toc/2045-2322Abstract Rapid design, screening, and characterization of biorecognition elements (BREs) is essential for the development of diagnostic tests and antiviral therapeutics needed to combat the spread of viruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). To address this need, we developed a high-throughput pipeline combining in silico design of a peptide library specific for SARS-CoV-2 spike (S) protein and microarray screening to identify binding sequences. Our optimized microarray platform allowed the simultaneous screening of ~ 2.5 k peptides and rapid identification of binding sequences resulting in selection of four peptides with nanomolar affinity to the SARS-CoV-2 S protein. Finally, we demonstrated the successful integration of one of the top peptides into an electrochemical sensor with a clinically relevant limit of detection for S protein in spiked saliva. Our results demonstrate the utility of this novel pipeline for the selection of peptide BREs in response to the SARS-CoV-2 pandemic, and the broader application of such a platform in response to future viral threats.Monica WolfeSean WebbYaroslav ChushakRachel KrabacherYi LiuNathan SwamiSvetlana HarbaughJorge ChávezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Monica Wolfe Sean Webb Yaroslav Chushak Rachel Krabacher Yi Liu Nathan Swami Svetlana Harbaugh Jorge Chávez A high-throughput pipeline for design and selection of peptides targeting the SARS-Cov-2 Spike protein
description	Abstract Rapid design, screening, and characterization of biorecognition elements (BREs) is essential for the development of diagnostic tests and antiviral therapeutics needed to combat the spread of viruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). To address this need, we developed a high-throughput pipeline combining in silico design of a peptide library specific for SARS-CoV-2 spike (S) protein and microarray screening to identify binding sequences. Our optimized microarray platform allowed the simultaneous screening of ~ 2.5 k peptides and rapid identification of binding sequences resulting in selection of four peptides with nanomolar affinity to the SARS-CoV-2 S protein. Finally, we demonstrated the successful integration of one of the top peptides into an electrochemical sensor with a clinically relevant limit of detection for S protein in spiked saliva. Our results demonstrate the utility of this novel pipeline for the selection of peptide BREs in response to the SARS-CoV-2 pandemic, and the broader application of such a platform in response to future viral threats.
format	article
author	Monica Wolfe Sean Webb Yaroslav Chushak Rachel Krabacher Yi Liu Nathan Swami Svetlana Harbaugh Jorge Chávez
author_facet	Monica Wolfe Sean Webb Yaroslav Chushak Rachel Krabacher Yi Liu Nathan Swami Svetlana Harbaugh Jorge Chávez
author_sort	Monica Wolfe
title	A high-throughput pipeline for design and selection of peptides targeting the SARS-Cov-2 Spike protein
title_short	A high-throughput pipeline for design and selection of peptides targeting the SARS-Cov-2 Spike protein
title_full	A high-throughput pipeline for design and selection of peptides targeting the SARS-Cov-2 Spike protein
title_fullStr	A high-throughput pipeline for design and selection of peptides targeting the SARS-Cov-2 Spike protein
title_full_unstemmed	A high-throughput pipeline for design and selection of peptides targeting the SARS-Cov-2 Spike protein
title_sort	high-throughput pipeline for design and selection of peptides targeting the sars-cov-2 spike protein
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/179bddfe608341f48fcb672122105c86
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A high-throughput pipeline for design and selection of peptides targeting the SARS-Cov-2 Spike protein

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