Locating human splenic capillary sheaths in virtual reality

Abstract Stromal capillary sheath cells in human spleens strongly express CD271, the low affinity nerve growth factor receptor p75. Serial sections of a representative adult human spleen were double-stained for CD271 versus smooth muscle alpha actin (SMA) plus CD34 to visualise capillary sheaths, th...

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Autores principales:	B. S. Steiniger, V. Wilhelmi, M. Berthold, M. Guthe, O. Lobachev
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Capillary Sheath Human Spleen Marginal Reticular Cells Marginal Metallophilic Macrophages (MMMs) Feeding Arterioles Medicine R Science Q
Acceso en línea:	https://doaj.org/article/17a6481e7809449f84909315cfa196fb
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spelling	oai:doaj.org-article:17a6481e7809449f84909315cfa196fb2021-12-02T11:40:25ZLocating human splenic capillary sheaths in virtual reality10.1038/s41598-018-34105-32045-2322https://doaj.org/article/17a6481e7809449f84909315cfa196fb2018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34105-3https://doaj.org/toc/2045-2322Abstract Stromal capillary sheath cells in human spleens strongly express CD271, the low affinity nerve growth factor receptor p75. Serial sections of a representative adult human spleen were double-stained for CD271 versus smooth muscle alpha actin (SMA) plus CD34 to visualise capillary sheaths, the arterial tree and endothelial cells by transmitted light. Preliminary three-dimensional (3D) reconstructions of single regions were inspected in virtual reality (VR). This method showed that a large number of CD271+ sheaths occur in a post-arteriolar position often surrounding capillaries located close to divisions of arterioles. The length and diameter of capillary sheaths are rather heterogeneous. Long sheaths were observed to accompany one or two generations of capillary branches. We hypothesise that human splenic capillary sheaths may attract recirculating B-lymphocytes from the open circulation of the red pulp to start their migration into white pulp follicles along branches of the arterial tree. In addition, they may provide sites of interaction among sheath macrophages and B-lymphocytes. Our innovative approach allows stringent quality control by inserting the original immunostained serial sections into the 3D model for viewing and annotation in VR. Longer series of sections will allow to unequivocally localise most of the capillary sheaths in a given volume.B. S. SteinigerV. WilhelmiM. BertholdM. GutheO. LobachevNature PortfolioarticleCapillary SheathHuman SpleenMarginal Reticular CellsMarginal Metallophilic Macrophages (MMMs)Feeding ArteriolesMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Capillary Sheath Human Spleen Marginal Reticular Cells Marginal Metallophilic Macrophages (MMMs) Feeding Arterioles Medicine R Science Q
spellingShingle	Capillary Sheath Human Spleen Marginal Reticular Cells Marginal Metallophilic Macrophages (MMMs) Feeding Arterioles Medicine R Science Q B. S. Steiniger V. Wilhelmi M. Berthold M. Guthe O. Lobachev Locating human splenic capillary sheaths in virtual reality
description	Abstract Stromal capillary sheath cells in human spleens strongly express CD271, the low affinity nerve growth factor receptor p75. Serial sections of a representative adult human spleen were double-stained for CD271 versus smooth muscle alpha actin (SMA) plus CD34 to visualise capillary sheaths, the arterial tree and endothelial cells by transmitted light. Preliminary three-dimensional (3D) reconstructions of single regions were inspected in virtual reality (VR). This method showed that a large number of CD271+ sheaths occur in a post-arteriolar position often surrounding capillaries located close to divisions of arterioles. The length and diameter of capillary sheaths are rather heterogeneous. Long sheaths were observed to accompany one or two generations of capillary branches. We hypothesise that human splenic capillary sheaths may attract recirculating B-lymphocytes from the open circulation of the red pulp to start their migration into white pulp follicles along branches of the arterial tree. In addition, they may provide sites of interaction among sheath macrophages and B-lymphocytes. Our innovative approach allows stringent quality control by inserting the original immunostained serial sections into the 3D model for viewing and annotation in VR. Longer series of sections will allow to unequivocally localise most of the capillary sheaths in a given volume.
format	article
author	B. S. Steiniger V. Wilhelmi M. Berthold M. Guthe O. Lobachev
author_facet	B. S. Steiniger V. Wilhelmi M. Berthold M. Guthe O. Lobachev
author_sort	B. S. Steiniger
title	Locating human splenic capillary sheaths in virtual reality
title_short	Locating human splenic capillary sheaths in virtual reality
title_full	Locating human splenic capillary sheaths in virtual reality
title_fullStr	Locating human splenic capillary sheaths in virtual reality
title_full_unstemmed	Locating human splenic capillary sheaths in virtual reality
title_sort	locating human splenic capillary sheaths in virtual reality
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/17a6481e7809449f84909315cfa196fb
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_version_	1718395625773465600

Locating human splenic capillary sheaths in virtual reality

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