Role of flagella and type four pili in the co-migration of Burkholderia terrae BS001 with fungal hyphae through soil

Abstract Burkholderia terrae BS001 has previously been found to be able to disperse along with growing fungal hyphae in soil, with the type-3 secretion system having a supportive role in this movement. In this study, we focus on the role of two motility- and adherence-associated appendages, i.e. typ...

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Autores principales:	Pu Yang, Miaozhi Zhang, Jan Dirk van Elsas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/17b01da765774e218b435ad2332ea202
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spelling	oai:doaj.org-article:17b01da765774e218b435ad2332ea2022021-12-02T15:05:43ZRole of flagella and type four pili in the co-migration of Burkholderia terrae BS001 with fungal hyphae through soil10.1038/s41598-017-02959-82045-2322https://doaj.org/article/17b01da765774e218b435ad2332ea2022017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02959-8https://doaj.org/toc/2045-2322Abstract Burkholderia terrae BS001 has previously been found to be able to disperse along with growing fungal hyphae in soil, with the type-3 secretion system having a supportive role in this movement. In this study, we focus on the role of two motility- and adherence-associated appendages, i.e. type-4 pili (T4P) and flagella. Electron microcopy and motility testing revealed that strain BS001 produces polar flagella and can swim on semi-solid R2A agar. Flagellum- and T4P-negative mutants were then constructed to examine the ecological roles of the respective systems. Both in liquid media and on swimming agar, the mutant strains showed similar fitness to the wild-type strain in mixed culture. The flagellar mutant had completely lost its flagella, as well as its swimming capacity. It also lost its co-migration ability with two soil-exploring fungi, Lyophyllum sp. strain Karsten and Trichoderma asperellum 302, in soil microcosms. In contrast, the T4P mutant showed reduced surface twitching motility, whereas its co-migration ability in competition with the wild-type strain was slightly reduced. We conclude that the co-migration of strain BS001 with fungal hyphae through soil is dependent on the presence of functional flagella conferring swimming motility, with the T4P system having a minor effect.Pu YangMiaozhi ZhangJan Dirk van ElsasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pu Yang Miaozhi Zhang Jan Dirk van Elsas Role of flagella and type four pili in the co-migration of Burkholderia terrae BS001 with fungal hyphae through soil
description	Abstract Burkholderia terrae BS001 has previously been found to be able to disperse along with growing fungal hyphae in soil, with the type-3 secretion system having a supportive role in this movement. In this study, we focus on the role of two motility- and adherence-associated appendages, i.e. type-4 pili (T4P) and flagella. Electron microcopy and motility testing revealed that strain BS001 produces polar flagella and can swim on semi-solid R2A agar. Flagellum- and T4P-negative mutants were then constructed to examine the ecological roles of the respective systems. Both in liquid media and on swimming agar, the mutant strains showed similar fitness to the wild-type strain in mixed culture. The flagellar mutant had completely lost its flagella, as well as its swimming capacity. It also lost its co-migration ability with two soil-exploring fungi, Lyophyllum sp. strain Karsten and Trichoderma asperellum 302, in soil microcosms. In contrast, the T4P mutant showed reduced surface twitching motility, whereas its co-migration ability in competition with the wild-type strain was slightly reduced. We conclude that the co-migration of strain BS001 with fungal hyphae through soil is dependent on the presence of functional flagella conferring swimming motility, with the T4P system having a minor effect.
format	article
author	Pu Yang Miaozhi Zhang Jan Dirk van Elsas
author_facet	Pu Yang Miaozhi Zhang Jan Dirk van Elsas
author_sort	Pu Yang
title	Role of flagella and type four pili in the co-migration of Burkholderia terrae BS001 with fungal hyphae through soil
title_short	Role of flagella and type four pili in the co-migration of Burkholderia terrae BS001 with fungal hyphae through soil
title_full	Role of flagella and type four pili in the co-migration of Burkholderia terrae BS001 with fungal hyphae through soil
title_fullStr	Role of flagella and type four pili in the co-migration of Burkholderia terrae BS001 with fungal hyphae through soil
title_full_unstemmed	Role of flagella and type four pili in the co-migration of Burkholderia terrae BS001 with fungal hyphae through soil
title_sort	role of flagella and type four pili in the co-migration of burkholderia terrae bs001 with fungal hyphae through soil
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/17b01da765774e218b435ad2332ea202
work_keys_str_mv	AT puyang roleofflagellaandtypefourpiliinthecomigrationofburkholderiaterraebs001withfungalhyphaethroughsoil AT miaozhizhang roleofflagellaandtypefourpiliinthecomigrationofburkholderiaterraebs001withfungalhyphaethroughsoil AT jandirkvanelsas roleofflagellaandtypefourpiliinthecomigrationofburkholderiaterraebs001withfungalhyphaethroughsoil
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Role of flagella and type four pili in the co-migration of Burkholderia terrae BS001 with fungal hyphae through soil

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