Effects of common Gram-negative pathogens causing male genitourinary-tract infections on human sperm functions

Abstract Male genitourinary tract (MGT) bacterial infections are considered responsible for 15% of male infertility, but the mechanisms underlying decreased semen quality are poorly known. We evaluated in vitro the effect of strains of Gram-negative uropathogenic species (two E.coli strains, three K...

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Autores principales:	Sara Marchiani, Ilaria Baccani, Lara Tamburrino, Giorgio Mattiuz, Sabrina Nicolò, Chiara Bonaiuto, Carmen Panico, Linda Vignozzi, Alberto Antonelli, Gianmaria Rossolini, Maria Torcia, Elisabetta Baldi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/17b508fd85e04bfe803e658cba9df1f0
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spelling	oai:doaj.org-article:17b508fd85e04bfe803e658cba9df1f02021-12-02T18:51:35ZEffects of common Gram-negative pathogens causing male genitourinary-tract infections on human sperm functions10.1038/s41598-021-98710-52045-2322https://doaj.org/article/17b508fd85e04bfe803e658cba9df1f02021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98710-5https://doaj.org/toc/2045-2322Abstract Male genitourinary tract (MGT) bacterial infections are considered responsible for 15% of male infertility, but the mechanisms underlying decreased semen quality are poorly known. We evaluated in vitro the effect of strains of Gram-negative uropathogenic species (two E.coli strains, three K. pneumoniae strains, P. aeruginosa and E. cloacae) on motility, viability, mitochondrial oxidative status, DNA fragmentation and caspase activity of human spermatozoa. All strains, except P. aeruginosa, reduced significantly sperm motility, with variable effects. Sperm Immobilizing Factor (SIF) was largely responsible for deteriorating effects on sperm motility of E. coli strains since they were completely reverted by knockout of SIF coding recX gene. Sequence alignment for RecX showed the presence of high homologous sequences in K. pneumoniae and E. cloacae but not in P. aeruginosa. These results suggest that, in addition to E.coli, other common uropathogenic Gram-negative bacteria affect sperm motility through RecX products. In addition to sperm motility, the E. coli strain ATCC 35218 also affected sperm viability, and induced caspase activity, oxidative stress and DNA fragmentation suggesting an interspecies variability in the amount and/or type of the produced spermatotoxic factors. In general, our results highlight the need for a careful evaluation of semen infections in the diagnostic process of the infertile man.Sara MarchianiIlaria BaccaniLara TamburrinoGiorgio MattiuzSabrina NicolòChiara BonaiutoCarmen PanicoLinda VignozziAlberto AntonelliGianmaria RossoliniMaria TorciaElisabetta BaldiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sara Marchiani Ilaria Baccani Lara Tamburrino Giorgio Mattiuz Sabrina Nicolò Chiara Bonaiuto Carmen Panico Linda Vignozzi Alberto Antonelli Gianmaria Rossolini Maria Torcia Elisabetta Baldi Effects of common Gram-negative pathogens causing male genitourinary-tract infections on human sperm functions
description	Abstract Male genitourinary tract (MGT) bacterial infections are considered responsible for 15% of male infertility, but the mechanisms underlying decreased semen quality are poorly known. We evaluated in vitro the effect of strains of Gram-negative uropathogenic species (two E.coli strains, three K. pneumoniae strains, P. aeruginosa and E. cloacae) on motility, viability, mitochondrial oxidative status, DNA fragmentation and caspase activity of human spermatozoa. All strains, except P. aeruginosa, reduced significantly sperm motility, with variable effects. Sperm Immobilizing Factor (SIF) was largely responsible for deteriorating effects on sperm motility of E. coli strains since they were completely reverted by knockout of SIF coding recX gene. Sequence alignment for RecX showed the presence of high homologous sequences in K. pneumoniae and E. cloacae but not in P. aeruginosa. These results suggest that, in addition to E.coli, other common uropathogenic Gram-negative bacteria affect sperm motility through RecX products. In addition to sperm motility, the E. coli strain ATCC 35218 also affected sperm viability, and induced caspase activity, oxidative stress and DNA fragmentation suggesting an interspecies variability in the amount and/or type of the produced spermatotoxic factors. In general, our results highlight the need for a careful evaluation of semen infections in the diagnostic process of the infertile man.
format	article
author	Sara Marchiani Ilaria Baccani Lara Tamburrino Giorgio Mattiuz Sabrina Nicolò Chiara Bonaiuto Carmen Panico Linda Vignozzi Alberto Antonelli Gianmaria Rossolini Maria Torcia Elisabetta Baldi
author_facet	Sara Marchiani Ilaria Baccani Lara Tamburrino Giorgio Mattiuz Sabrina Nicolò Chiara Bonaiuto Carmen Panico Linda Vignozzi Alberto Antonelli Gianmaria Rossolini Maria Torcia Elisabetta Baldi
author_sort	Sara Marchiani
title	Effects of common Gram-negative pathogens causing male genitourinary-tract infections on human sperm functions
title_short	Effects of common Gram-negative pathogens causing male genitourinary-tract infections on human sperm functions
title_full	Effects of common Gram-negative pathogens causing male genitourinary-tract infections on human sperm functions
title_fullStr	Effects of common Gram-negative pathogens causing male genitourinary-tract infections on human sperm functions
title_full_unstemmed	Effects of common Gram-negative pathogens causing male genitourinary-tract infections on human sperm functions
title_sort	effects of common gram-negative pathogens causing male genitourinary-tract infections on human sperm functions
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/17b508fd85e04bfe803e658cba9df1f0
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Effects of common Gram-negative pathogens causing male genitourinary-tract infections on human sperm functions

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