High-throughput sequencing reveals genetic determinants associated with antibiotic resistance in Campylobacter spp. from farm-to-fork.

Campylobacter species are one of the most common causative agents of gastroenteritis worldwide. Resistance against quinolone and macrolide antimicrobials, the most commonly used therapeutic options, poses a serious risk for campylobacteriosis treatment. Owing to whole genome sequencing advancements...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shaimaa F Mouftah, José F Cobo-Díaz, Avelino Álvarez-Ordóñez, Menattallah Elserafy, Nehal A Saif, Asmaa Sadat, Ayman El-Shibiny, Mohamed Elhadidy
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/17b557cfa49a4ba69ea124044262fa1c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:17b557cfa49a4ba69ea124044262fa1c
record_format	dspace
spelling	oai:doaj.org-article:17b557cfa49a4ba69ea124044262fa1c2021-12-02T20:10:03ZHigh-throughput sequencing reveals genetic determinants associated with antibiotic resistance in Campylobacter spp. from farm-to-fork.1932-620310.1371/journal.pone.0253797https://doaj.org/article/17b557cfa49a4ba69ea124044262fa1c2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253797https://doaj.org/toc/1932-6203Campylobacter species are one of the most common causative agents of gastroenteritis worldwide. Resistance against quinolone and macrolide antimicrobials, the most commonly used therapeutic options, poses a serious risk for campylobacteriosis treatment. Owing to whole genome sequencing advancements for rapid detection of antimicrobial resistance mechanisms, phenotypic and genotypic resistance trends along the "farm-to-fork" continuum can be determined. Here, we examined the resistance trends in 111 Campylobacter isolates (90 C. jejuni and 21 C. coli) recovered from clinical samples, commercial broiler carcasses and dairy products in Cairo, Egypt. Multidrug resistance (MDR) was observed in 10% of the isolates, mostly from C. coli. The prevalence of MDR was the highest in isolates collected from broiler carcasses (13.3%), followed by clinical isolates (10.5%), and finally isolates from dairy products (4%). The highest proportion of antimicrobial resistance in both species was against quinolones (ciprofloxacin and/or nalidixic acid) (68.4%), followed by tetracycline (51.3%), then erythromycin (12.6%) and aminoglycosides (streptomycin and/or gentamicin) (5.4%). Similar resistance rates were observed for quinolones, tetracycline, and erythromycin among isolates recovered from broiler carcasses and clinical samples highlighting the contribution of food of animal sources to human illness. Significant associations between phenotypic resistance and putative gene mutations was observed, with a high prevalence of the gyrA T86I substitution among quinolone resistant isolates, tet(O), tet(W), and tet(32) among tetracycline resistant isolates, and 23S rRNA A2075G and A2074T mutations among erythromycin resistant isolates. Emergence of resistance was attributed to the dissemination of resistance genes among various lineages, with the dominance of distinctive clones. For example, sub-lineages of CC828 in C. coli and CC21 in C. jejuni and the genetically related clonal complexes 'CC206 and CC48' and 'CC464, CC353, CC354, CC574', respectively, propagated across different niches sharing semi-homogenous resistance patterns.Shaimaa F MouftahJosé F Cobo-DíazAvelino Álvarez-OrdóñezMenattallah ElserafyNehal A SaifAsmaa SadatAyman El-ShibinyMohamed ElhadidyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0253797 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shaimaa F Mouftah José F Cobo-Díaz Avelino Álvarez-Ordóñez Menattallah Elserafy Nehal A Saif Asmaa Sadat Ayman El-Shibiny Mohamed Elhadidy High-throughput sequencing reveals genetic determinants associated with antibiotic resistance in Campylobacter spp. from farm-to-fork.
description	Campylobacter species are one of the most common causative agents of gastroenteritis worldwide. Resistance against quinolone and macrolide antimicrobials, the most commonly used therapeutic options, poses a serious risk for campylobacteriosis treatment. Owing to whole genome sequencing advancements for rapid detection of antimicrobial resistance mechanisms, phenotypic and genotypic resistance trends along the "farm-to-fork" continuum can be determined. Here, we examined the resistance trends in 111 Campylobacter isolates (90 C. jejuni and 21 C. coli) recovered from clinical samples, commercial broiler carcasses and dairy products in Cairo, Egypt. Multidrug resistance (MDR) was observed in 10% of the isolates, mostly from C. coli. The prevalence of MDR was the highest in isolates collected from broiler carcasses (13.3%), followed by clinical isolates (10.5%), and finally isolates from dairy products (4%). The highest proportion of antimicrobial resistance in both species was against quinolones (ciprofloxacin and/or nalidixic acid) (68.4%), followed by tetracycline (51.3%), then erythromycin (12.6%) and aminoglycosides (streptomycin and/or gentamicin) (5.4%). Similar resistance rates were observed for quinolones, tetracycline, and erythromycin among isolates recovered from broiler carcasses and clinical samples highlighting the contribution of food of animal sources to human illness. Significant associations between phenotypic resistance and putative gene mutations was observed, with a high prevalence of the gyrA T86I substitution among quinolone resistant isolates, tet(O), tet(W), and tet(32) among tetracycline resistant isolates, and 23S rRNA A2075G and A2074T mutations among erythromycin resistant isolates. Emergence of resistance was attributed to the dissemination of resistance genes among various lineages, with the dominance of distinctive clones. For example, sub-lineages of CC828 in C. coli and CC21 in C. jejuni and the genetically related clonal complexes 'CC206 and CC48' and 'CC464, CC353, CC354, CC574', respectively, propagated across different niches sharing semi-homogenous resistance patterns.
format	article
author	Shaimaa F Mouftah José F Cobo-Díaz Avelino Álvarez-Ordóñez Menattallah Elserafy Nehal A Saif Asmaa Sadat Ayman El-Shibiny Mohamed Elhadidy
author_facet	Shaimaa F Mouftah José F Cobo-Díaz Avelino Álvarez-Ordóñez Menattallah Elserafy Nehal A Saif Asmaa Sadat Ayman El-Shibiny Mohamed Elhadidy
author_sort	Shaimaa F Mouftah
title	High-throughput sequencing reveals genetic determinants associated with antibiotic resistance in Campylobacter spp. from farm-to-fork.
title_short	High-throughput sequencing reveals genetic determinants associated with antibiotic resistance in Campylobacter spp. from farm-to-fork.
title_full	High-throughput sequencing reveals genetic determinants associated with antibiotic resistance in Campylobacter spp. from farm-to-fork.
title_fullStr	High-throughput sequencing reveals genetic determinants associated with antibiotic resistance in Campylobacter spp. from farm-to-fork.
title_full_unstemmed	High-throughput sequencing reveals genetic determinants associated with antibiotic resistance in Campylobacter spp. from farm-to-fork.
title_sort	high-throughput sequencing reveals genetic determinants associated with antibiotic resistance in campylobacter spp. from farm-to-fork.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/17b557cfa49a4ba69ea124044262fa1c
work_keys_str_mv	AT shaimaafmouftah highthroughputsequencingrevealsgeneticdeterminantsassociatedwithantibioticresistanceincampylobactersppfromfarmtofork AT josefcobodiaz highthroughputsequencingrevealsgeneticdeterminantsassociatedwithantibioticresistanceincampylobactersppfromfarmtofork AT avelinoalvarezordonez highthroughputsequencingrevealsgeneticdeterminantsassociatedwithantibioticresistanceincampylobactersppfromfarmtofork AT menattallahelserafy highthroughputsequencingrevealsgeneticdeterminantsassociatedwithantibioticresistanceincampylobactersppfromfarmtofork AT nehalasaif highthroughputsequencingrevealsgeneticdeterminantsassociatedwithantibioticresistanceincampylobactersppfromfarmtofork AT asmaasadat highthroughputsequencingrevealsgeneticdeterminantsassociatedwithantibioticresistanceincampylobactersppfromfarmtofork AT aymanelshibiny highthroughputsequencingrevealsgeneticdeterminantsassociatedwithantibioticresistanceincampylobactersppfromfarmtofork AT mohamedelhadidy highthroughputsequencingrevealsgeneticdeterminantsassociatedwithantibioticresistanceincampylobactersppfromfarmtofork
_version_	1718374986097360896

High-throughput sequencing reveals genetic determinants associated with antibiotic resistance in Campylobacter spp. from farm-to-fork.

Ejemplares similares