Bioinspired Synthesis of ZnO@polydopamine/Au for Label-Free Photoelectrochemical Immunoassay of Amyloid-β Protein

Amyloid-β protein (Aβ) is an important biomarker and plays a key role in the early stage of Alzheimer’s disease (AD). Here, an ultrasensitive photoelectrochemical (PEC) sensor based on ZnO@polydopamine/Au nanocomposites was constructed for quantitative detection of Aβ. In this sensing system, the Zn...

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Autores principales:	Guangli He, Yue Zhou, Mifang Li, Yanzhen Guo, Hang Yin, Baocheng Yang, Shouren Zhang, Yibiao Liu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	photoelectrochemical sensor ZnO polydopamine gold nanoparticles Alzheimer’s disease Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/17ba753d4d754fc6a3e98d3b66bfdf62
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spelling	oai:doaj.org-article:17ba753d4d754fc6a3e98d3b66bfdf622021-11-16T07:48:21ZBioinspired Synthesis of ZnO@polydopamine/Au for Label-Free Photoelectrochemical Immunoassay of Amyloid-β Protein2296-418510.3389/fbioe.2021.777344https://doaj.org/article/17ba753d4d754fc6a3e98d3b66bfdf622021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.777344/fullhttps://doaj.org/toc/2296-4185Amyloid-β protein (Aβ) is an important biomarker and plays a key role in the early stage of Alzheimer’s disease (AD). Here, an ultrasensitive photoelectrochemical (PEC) sensor based on ZnO@polydopamine/Au nanocomposites was constructed for quantitative detection of Aβ. In this sensing system, the ZnO nanorod array decorated with PDA films and gold nanoparticles (Au NPs) have excellent visible-light activity. The PDA film was used as a sensitizer for charge separation, and it also was used for antibody binding. Moreover, Au NPs were loaded on the surface of PDA film by in situ deposition, which further improved the charge transfer efficiency and the PEC activity in visible light due to the localized surface plasmon resonance effect of Au NPs. Therefore, in ZnO@polydopamine/Au nanocomposites, a significantly enhanced photocurrent response was obtained on this photoelectrode, which provides a good and reliable signal for early detection of AD. Under the optimized conditions, the PEC immunosensor displayed a wide linear range from 1 pg/mL to 100 ng/mL and a low detection limit of 0.26 pg/mL. In addition, this PEC immunosensor also presented good selectivity, stability, and reproducibility. This work may provide a promising point-of-care testing method toward advanced PEC immunoassays for AD biomarkers.Guangli HeYue ZhouMifang LiYanzhen GuoHang YinBaocheng YangShouren ZhangYibiao LiuFrontiers Media S.A.articlephotoelectrochemical sensorZnOpolydopaminegold nanoparticlesAlzheimer’s diseaseBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	photoelectrochemical sensor ZnO polydopamine gold nanoparticles Alzheimer’s disease Biotechnology TP248.13-248.65
spellingShingle	photoelectrochemical sensor ZnO polydopamine gold nanoparticles Alzheimer’s disease Biotechnology TP248.13-248.65 Guangli He Yue Zhou Mifang Li Yanzhen Guo Hang Yin Baocheng Yang Shouren Zhang Yibiao Liu Bioinspired Synthesis of ZnO@polydopamine/Au for Label-Free Photoelectrochemical Immunoassay of Amyloid-β Protein
description	Amyloid-β protein (Aβ) is an important biomarker and plays a key role in the early stage of Alzheimer’s disease (AD). Here, an ultrasensitive photoelectrochemical (PEC) sensor based on ZnO@polydopamine/Au nanocomposites was constructed for quantitative detection of Aβ. In this sensing system, the ZnO nanorod array decorated with PDA films and gold nanoparticles (Au NPs) have excellent visible-light activity. The PDA film was used as a sensitizer for charge separation, and it also was used for antibody binding. Moreover, Au NPs were loaded on the surface of PDA film by in situ deposition, which further improved the charge transfer efficiency and the PEC activity in visible light due to the localized surface plasmon resonance effect of Au NPs. Therefore, in ZnO@polydopamine/Au nanocomposites, a significantly enhanced photocurrent response was obtained on this photoelectrode, which provides a good and reliable signal for early detection of AD. Under the optimized conditions, the PEC immunosensor displayed a wide linear range from 1 pg/mL to 100 ng/mL and a low detection limit of 0.26 pg/mL. In addition, this PEC immunosensor also presented good selectivity, stability, and reproducibility. This work may provide a promising point-of-care testing method toward advanced PEC immunoassays for AD biomarkers.
format	article
author	Guangli He Yue Zhou Mifang Li Yanzhen Guo Hang Yin Baocheng Yang Shouren Zhang Yibiao Liu
author_facet	Guangli He Yue Zhou Mifang Li Yanzhen Guo Hang Yin Baocheng Yang Shouren Zhang Yibiao Liu
author_sort	Guangli He
title	Bioinspired Synthesis of ZnO@polydopamine/Au for Label-Free Photoelectrochemical Immunoassay of Amyloid-β Protein
title_short	Bioinspired Synthesis of ZnO@polydopamine/Au for Label-Free Photoelectrochemical Immunoassay of Amyloid-β Protein
title_full	Bioinspired Synthesis of ZnO@polydopamine/Au for Label-Free Photoelectrochemical Immunoassay of Amyloid-β Protein
title_fullStr	Bioinspired Synthesis of ZnO@polydopamine/Au for Label-Free Photoelectrochemical Immunoassay of Amyloid-β Protein
title_full_unstemmed	Bioinspired Synthesis of ZnO@polydopamine/Au for Label-Free Photoelectrochemical Immunoassay of Amyloid-β Protein
title_sort	bioinspired synthesis of zno@polydopamine/au for label-free photoelectrochemical immunoassay of amyloid-β protein
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/17ba753d4d754fc6a3e98d3b66bfdf62
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Bioinspired Synthesis of ZnO@polydopamine/Au for Label-Free Photoelectrochemical Immunoassay of Amyloid-β Protein

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