LGR5 is a negative regulator of tumourigenicity, antagonizes Wnt signalling and regulates cell adhesion in colorectal cancer cell lines.

<h4>Background</h4>LGR5 (Leucine-rich repeat-containing G-protein coupled receptor 5) is the most established marker for intestinal stem cells. Mouse models show that LGR5+ cells are the cells of origin of intestinal cancer, and LGR5 expression is elevated in human colorectal cancers, ho...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Francesca Walker, Hui-Hua Zhang, Annalisa Odorizzi, Antony W Burgess
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/17c6b9b10c5045ddaa12ea2b61b46ec1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:17c6b9b10c5045ddaa12ea2b61b46ec1
record_format	dspace
spelling	oai:doaj.org-article:17c6b9b10c5045ddaa12ea2b61b46ec12021-11-18T06:49:09ZLGR5 is a negative regulator of tumourigenicity, antagonizes Wnt signalling and regulates cell adhesion in colorectal cancer cell lines.1932-620310.1371/journal.pone.0022733https://doaj.org/article/17c6b9b10c5045ddaa12ea2b61b46ec12011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21829496/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>LGR5 (Leucine-rich repeat-containing G-protein coupled receptor 5) is the most established marker for intestinal stem cells. Mouse models show that LGR5+ cells are the cells of origin of intestinal cancer, and LGR5 expression is elevated in human colorectal cancers, however very little is known about LGR5 function or its contribution to the stem cell phenotype and to colorectal cancer.<h4>Principal findings</h4>We have modulated the expression of LGR5 by RNAi (inhibitory RNAs) or overexpression in colorectal cancer cell lines. Paradoxically, ablation of LGR5 induces increased invasion and anchorage-independent growth, and enhances tumourigenicity in xenografts experiments. Conversely, overexpression of LGR5 augments cell adhesion, reduces clonogenicity and attenuates tumourigenicity. Expression profiling revealed enhanced wnt signalling and upregulation of EMT genes upon knockdown of LGR5, with opposite changes in LGR5 overexpressing cells. These findings suggest that LGR5 is important in restricting stem cells to their niche, and that loss of LGR5 concomitant with activated wnt signalling may contribute to the invasive phenotype of colorectal carcinomas.Francesca WalkerHui-Hua ZhangAnnalisa OdorizziAntony W BurgessPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e22733 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Francesca Walker Hui-Hua Zhang Annalisa Odorizzi Antony W Burgess LGR5 is a negative regulator of tumourigenicity, antagonizes Wnt signalling and regulates cell adhesion in colorectal cancer cell lines.
description	<h4>Background</h4>LGR5 (Leucine-rich repeat-containing G-protein coupled receptor 5) is the most established marker for intestinal stem cells. Mouse models show that LGR5+ cells are the cells of origin of intestinal cancer, and LGR5 expression is elevated in human colorectal cancers, however very little is known about LGR5 function or its contribution to the stem cell phenotype and to colorectal cancer.<h4>Principal findings</h4>We have modulated the expression of LGR5 by RNAi (inhibitory RNAs) or overexpression in colorectal cancer cell lines. Paradoxically, ablation of LGR5 induces increased invasion and anchorage-independent growth, and enhances tumourigenicity in xenografts experiments. Conversely, overexpression of LGR5 augments cell adhesion, reduces clonogenicity and attenuates tumourigenicity. Expression profiling revealed enhanced wnt signalling and upregulation of EMT genes upon knockdown of LGR5, with opposite changes in LGR5 overexpressing cells. These findings suggest that LGR5 is important in restricting stem cells to their niche, and that loss of LGR5 concomitant with activated wnt signalling may contribute to the invasive phenotype of colorectal carcinomas.
format	article
author	Francesca Walker Hui-Hua Zhang Annalisa Odorizzi Antony W Burgess
author_facet	Francesca Walker Hui-Hua Zhang Annalisa Odorizzi Antony W Burgess
author_sort	Francesca Walker
title	LGR5 is a negative regulator of tumourigenicity, antagonizes Wnt signalling and regulates cell adhesion in colorectal cancer cell lines.
title_short	LGR5 is a negative regulator of tumourigenicity, antagonizes Wnt signalling and regulates cell adhesion in colorectal cancer cell lines.
title_full	LGR5 is a negative regulator of tumourigenicity, antagonizes Wnt signalling and regulates cell adhesion in colorectal cancer cell lines.
title_fullStr	LGR5 is a negative regulator of tumourigenicity, antagonizes Wnt signalling and regulates cell adhesion in colorectal cancer cell lines.
title_full_unstemmed	LGR5 is a negative regulator of tumourigenicity, antagonizes Wnt signalling and regulates cell adhesion in colorectal cancer cell lines.
title_sort	lgr5 is a negative regulator of tumourigenicity, antagonizes wnt signalling and regulates cell adhesion in colorectal cancer cell lines.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/17c6b9b10c5045ddaa12ea2b61b46ec1
work_keys_str_mv	AT francescawalker lgr5isanegativeregulatoroftumourigenicityantagonizeswntsignallingandregulatescelladhesionincolorectalcancercelllines AT huihuazhang lgr5isanegativeregulatoroftumourigenicityantagonizeswntsignallingandregulatescelladhesionincolorectalcancercelllines AT annalisaodorizzi lgr5isanegativeregulatoroftumourigenicityantagonizeswntsignallingandregulatescelladhesionincolorectalcancercelllines AT antonywburgess lgr5isanegativeregulatoroftumourigenicityantagonizeswntsignallingandregulatescelladhesionincolorectalcancercelllines
_version_	1718424336288710656

LGR5 is a negative regulator of tumourigenicity, antagonizes Wnt signalling and regulates cell adhesion in colorectal cancer cell lines.

Ejemplares similares