Generation of functional eyes from pluripotent cells.

Pluripotent cells such as embryonic stem (ES) and induced pluripotent stem (iPS) cells are the starting point from which to generate organ specific cell types. For example, converting pluripotent cells to retinal cells could provide an opportunity to treat retinal injuries and degenerations. In this...

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Autores principales:	Andrea S Viczian, Eduardo C Solessio, Yung Lyou, Michael E Zuber
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/17d5aa042b354458b2bb383346ae07b3
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spelling	oai:doaj.org-article:17d5aa042b354458b2bb383346ae07b32021-11-25T05:34:04ZGeneration of functional eyes from pluripotent cells.1544-91731545-788510.1371/journal.pbio.1000174https://doaj.org/article/17d5aa042b354458b2bb383346ae07b32009-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19688031/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Pluripotent cells such as embryonic stem (ES) and induced pluripotent stem (iPS) cells are the starting point from which to generate organ specific cell types. For example, converting pluripotent cells to retinal cells could provide an opportunity to treat retinal injuries and degenerations. In this study, we used an in vivo strategy to determine if functional retinas could be generated from a defined population of pluripotent Xenopus laevis cells. Animal pole cells isolated from blastula stage embryos are pluripotent. Untreated, these cells formed only epidermis, when transplanted to either the flank or eye field. In contrast, misexpression of seven transcription factors induced the formation of retinal cell types. Induced retinal cells were committed to a retinal lineage as they formed eyes when transplanted to the flanks of developing embryos. When the endogenous eye field was replaced with induced retinal cells, they formed eyes that were molecularly, anatomically, and electrophysiologically similar to normal eyes. Importantly, induced eyes could guide a vision-based behavior. These results suggest the fate of pluripotent cells may be purposely altered to generate multipotent retinal progenitor cells, which differentiate into functional retinal cell classes and form a neural circuitry sufficient for vision.Andrea S ViczianEduardo C SolessioYung LyouMichael E ZuberPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 7, Iss 8, p e1000174 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Andrea S Viczian Eduardo C Solessio Yung Lyou Michael E Zuber Generation of functional eyes from pluripotent cells.
description	Pluripotent cells such as embryonic stem (ES) and induced pluripotent stem (iPS) cells are the starting point from which to generate organ specific cell types. For example, converting pluripotent cells to retinal cells could provide an opportunity to treat retinal injuries and degenerations. In this study, we used an in vivo strategy to determine if functional retinas could be generated from a defined population of pluripotent Xenopus laevis cells. Animal pole cells isolated from blastula stage embryos are pluripotent. Untreated, these cells formed only epidermis, when transplanted to either the flank or eye field. In contrast, misexpression of seven transcription factors induced the formation of retinal cell types. Induced retinal cells were committed to a retinal lineage as they formed eyes when transplanted to the flanks of developing embryos. When the endogenous eye field was replaced with induced retinal cells, they formed eyes that were molecularly, anatomically, and electrophysiologically similar to normal eyes. Importantly, induced eyes could guide a vision-based behavior. These results suggest the fate of pluripotent cells may be purposely altered to generate multipotent retinal progenitor cells, which differentiate into functional retinal cell classes and form a neural circuitry sufficient for vision.
format	article
author	Andrea S Viczian Eduardo C Solessio Yung Lyou Michael E Zuber
author_facet	Andrea S Viczian Eduardo C Solessio Yung Lyou Michael E Zuber
author_sort	Andrea S Viczian
title	Generation of functional eyes from pluripotent cells.
title_short	Generation of functional eyes from pluripotent cells.
title_full	Generation of functional eyes from pluripotent cells.
title_fullStr	Generation of functional eyes from pluripotent cells.
title_full_unstemmed	Generation of functional eyes from pluripotent cells.
title_sort	generation of functional eyes from pluripotent cells.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/17d5aa042b354458b2bb383346ae07b3
work_keys_str_mv	AT andreasviczian generationoffunctionaleyesfrompluripotentcells AT eduardocsolessio generationoffunctionaleyesfrompluripotentcells AT yunglyou generationoffunctionaleyesfrompluripotentcells AT michaelezuber generationoffunctionaleyesfrompluripotentcells
_version_	1718414561569144832

Generation of functional eyes from pluripotent cells.

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