Analysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics

Abstract. Abinawanto, Sophian A, Lestari R, Bowolaksono A, Efendi PS, Afnan R. 2019. Analysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics. Biodiversitas 20: 2004-2010. Insulin-like growth factor 1 (IGF-1) is defined as an essential gene fo...

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Autores principales: ABINAWANTO ABINAWANTO, ALFI SOPHIAN, RETNO LESTARI, ANOM BOWOLAKSONO, PIPIH SUNINGSIH EFENDI, RUDI AFNAN
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Publicado: MBI & UNS Solo 2019
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spelling oai:doaj.org-article:17da2aae5d0a4a2ba18ce0b0b9c511762021-11-21T21:42:27ZAnalysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics1412-033X2085-472210.13057/biodiv/d200729https://doaj.org/article/17da2aae5d0a4a2ba18ce0b0b9c511762019-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4112https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Abinawanto, Sophian A, Lestari R, Bowolaksono A, Efendi PS, Afnan R. 2019. Analysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics. Biodiversitas 20: 2004-2010. Insulin-like growth factor 1 (IGF-1) is defined as an essential gene for growth. Therefore, this research aims to identify the polymorphism of the IGF-1 gene from ayam ketawa with the dangdut and slow type vocal characteristics. Twenty male chickens were selected as an animal model. The sample consisted of 10 chickens with the dangdut type and 10 chickens with the slow type. The chickens were all obtained from five areas of the Pinrang district. The RFLP and HRM methods were selected to be used in this research. Results showed that the IGF-1 gene was 622 bp in length, while polymorphism analysis showed that the dangdut and slow type are homozygotic. The unrestricted DNA fragment indicated it after reaction of the restriction enzyme Pst1. Moreover, it confirmed that ayam ketawa with the dangdut or slow type had a similar sequence.ABINAWANTO ABINAWANTOALFI SOPHIANRETNO LESTARIANOM BOWOLAKSONOPIPIH SUNINGSIH EFENDIRUDI AFNANMBI & UNS Soloarticleayam ketawa, hrm, igf-1, polymorphism, rflpBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 7 (2019)
institution DOAJ
collection DOAJ
language EN
topic ayam ketawa, hrm, igf-1, polymorphism, rflp
Biology (General)
QH301-705.5
spellingShingle ayam ketawa, hrm, igf-1, polymorphism, rflp
Biology (General)
QH301-705.5
ABINAWANTO ABINAWANTO
ALFI SOPHIAN
RETNO LESTARI
ANOM BOWOLAKSONO
PIPIH SUNINGSIH EFENDI
RUDI AFNAN
Analysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics
description Abstract. Abinawanto, Sophian A, Lestari R, Bowolaksono A, Efendi PS, Afnan R. 2019. Analysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics. Biodiversitas 20: 2004-2010. Insulin-like growth factor 1 (IGF-1) is defined as an essential gene for growth. Therefore, this research aims to identify the polymorphism of the IGF-1 gene from ayam ketawa with the dangdut and slow type vocal characteristics. Twenty male chickens were selected as an animal model. The sample consisted of 10 chickens with the dangdut type and 10 chickens with the slow type. The chickens were all obtained from five areas of the Pinrang district. The RFLP and HRM methods were selected to be used in this research. Results showed that the IGF-1 gene was 622 bp in length, while polymorphism analysis showed that the dangdut and slow type are homozygotic. The unrestricted DNA fragment indicated it after reaction of the restriction enzyme Pst1. Moreover, it confirmed that ayam ketawa with the dangdut or slow type had a similar sequence.
format article
author ABINAWANTO ABINAWANTO
ALFI SOPHIAN
RETNO LESTARI
ANOM BOWOLAKSONO
PIPIH SUNINGSIH EFENDI
RUDI AFNAN
author_facet ABINAWANTO ABINAWANTO
ALFI SOPHIAN
RETNO LESTARI
ANOM BOWOLAKSONO
PIPIH SUNINGSIH EFENDI
RUDI AFNAN
author_sort ABINAWANTO ABINAWANTO
title Analysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics
title_short Analysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics
title_full Analysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics
title_fullStr Analysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics
title_full_unstemmed Analysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics
title_sort analysis of igf-1 gene in ayam ketawa (gallus gallus domesticus) with dangdut and slow type vocal characteristics
publisher MBI & UNS Solo
publishDate 2019
url https://doaj.org/article/17da2aae5d0a4a2ba18ce0b0b9c51176
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