Atomic Force Microscopy Demonstrates that <named-content content-type="genus-species">Candida glabrata</named-content> Uses Three Epa Proteins To Mediate Adhesion to Abiotic Surfaces

ABSTRACT The fungal pathogen Candida glabrata can cause both mucosal and disseminated infections. Cell adhesion, a key step in colonization and infection, depends in C. glabrata primarily on the Epa family of cell adhesion proteins. While Epa proteins have been documented to mediate specific adhesio...

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Autores principales:	Claire Valotteau, Valeria Prystopiuk, Brendan P. Cormack, Yves F. Dufrêne
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Candida glabrata adhesion AFM EPA Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/17dcd5c404b84f52b17a49c61323ea95
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spelling	oai:doaj.org-article:17dcd5c404b84f52b17a49c61323ea952021-11-15T15:22:20ZAtomic Force Microscopy Demonstrates that <named-content content-type="genus-species">Candida glabrata</named-content> Uses Three Epa Proteins To Mediate Adhesion to Abiotic Surfaces10.1128/mSphere.00277-192379-5042https://doaj.org/article/17dcd5c404b84f52b17a49c61323ea952019-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00277-19https://doaj.org/toc/2379-5042ABSTRACT The fungal pathogen Candida glabrata can cause both mucosal and disseminated infections. Cell adhesion, a key step in colonization and infection, depends in C. glabrata primarily on the Epa family of cell adhesion proteins. While Epa proteins have been documented to mediate specific adhesion to host glycans, some of them also promote nonspecific adhesion to abiotic surfaces, though this is incompletely understood. Here we address this issue using a combination of genetics and single-cell force measurements. By quantifying the forces driving the attachment of single C. glabrata cells to hydrophobic and hydrophilic substrates, we show that cell adhesion is strongly increased by loss of Sir-mediated silencing. Using a series of mutant strains lacking specific EPA genes, we demonstrate unexpectedly that three major Epa proteins, Epa1, Epa6, and Epa7, primarily contribute to both hydrophilic and hydrophobic interactions, suggesting a broad role for the Epa adhesins in mediating specific and nonspecific adherence and implicating Epa genes in biofilm formation on abiotic surfaces. IMPORTANCE Candida glabrata cell wall proteins mediate the attachment of C. glabrata to abiotic surfaces through molecular interactions that are poorly understood. Here, we study the forces engaged in Epa-dependent adhesion using single-cell techniques. Fungal adhesion to hydrophilic and hydrophobic substrates involves mainly three Epa proteins, suggesting a broad role for the Epa adhesins in mediating adherence. These proteins might represent a potential target for the development of innovative antifungal drugs.Claire ValotteauValeria PrystopiukBrendan P. CormackYves F. DufrêneAmerican Society for MicrobiologyarticleCandida glabrataadhesionAFMEPAMicrobiologyQR1-502ENmSphere, Vol 4, Iss 3 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Candida glabrata adhesion AFM EPA Microbiology QR1-502
spellingShingle	Candida glabrata adhesion AFM EPA Microbiology QR1-502 Claire Valotteau Valeria Prystopiuk Brendan P. Cormack Yves F. Dufrêne Atomic Force Microscopy Demonstrates that <named-content content-type="genus-species">Candida glabrata</named-content> Uses Three Epa Proteins To Mediate Adhesion to Abiotic Surfaces
description	ABSTRACT The fungal pathogen Candida glabrata can cause both mucosal and disseminated infections. Cell adhesion, a key step in colonization and infection, depends in C. glabrata primarily on the Epa family of cell adhesion proteins. While Epa proteins have been documented to mediate specific adhesion to host glycans, some of them also promote nonspecific adhesion to abiotic surfaces, though this is incompletely understood. Here we address this issue using a combination of genetics and single-cell force measurements. By quantifying the forces driving the attachment of single C. glabrata cells to hydrophobic and hydrophilic substrates, we show that cell adhesion is strongly increased by loss of Sir-mediated silencing. Using a series of mutant strains lacking specific EPA genes, we demonstrate unexpectedly that three major Epa proteins, Epa1, Epa6, and Epa7, primarily contribute to both hydrophilic and hydrophobic interactions, suggesting a broad role for the Epa adhesins in mediating specific and nonspecific adherence and implicating Epa genes in biofilm formation on abiotic surfaces. IMPORTANCE Candida glabrata cell wall proteins mediate the attachment of C. glabrata to abiotic surfaces through molecular interactions that are poorly understood. Here, we study the forces engaged in Epa-dependent adhesion using single-cell techniques. Fungal adhesion to hydrophilic and hydrophobic substrates involves mainly three Epa proteins, suggesting a broad role for the Epa adhesins in mediating adherence. These proteins might represent a potential target for the development of innovative antifungal drugs.
format	article
author	Claire Valotteau Valeria Prystopiuk Brendan P. Cormack Yves F. Dufrêne
author_facet	Claire Valotteau Valeria Prystopiuk Brendan P. Cormack Yves F. Dufrêne
author_sort	Claire Valotteau
title	Atomic Force Microscopy Demonstrates that <named-content content-type="genus-species">Candida glabrata</named-content> Uses Three Epa Proteins To Mediate Adhesion to Abiotic Surfaces
title_short	Atomic Force Microscopy Demonstrates that <named-content content-type="genus-species">Candida glabrata</named-content> Uses Three Epa Proteins To Mediate Adhesion to Abiotic Surfaces
title_full	Atomic Force Microscopy Demonstrates that <named-content content-type="genus-species">Candida glabrata</named-content> Uses Three Epa Proteins To Mediate Adhesion to Abiotic Surfaces
title_fullStr	Atomic Force Microscopy Demonstrates that <named-content content-type="genus-species">Candida glabrata</named-content> Uses Three Epa Proteins To Mediate Adhesion to Abiotic Surfaces
title_full_unstemmed	Atomic Force Microscopy Demonstrates that <named-content content-type="genus-species">Candida glabrata</named-content> Uses Three Epa Proteins To Mediate Adhesion to Abiotic Surfaces
title_sort	atomic force microscopy demonstrates that <named-content content-type="genus-species">candida glabrata</named-content> uses three epa proteins to mediate adhesion to abiotic surfaces
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/17dcd5c404b84f52b17a49c61323ea95
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Atomic Force Microscopy Demonstrates that <named-content content-type="genus-species">Candida glabrata</named-content> Uses Three Epa Proteins To Mediate Adhesion to Abiotic Surfaces

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