Pseudomonas syringae pv. syringae uses proteasome inhibitor syringolin A to colonize from wound infection sites.

Infection of plants by bacterial leaf pathogens at wound sites is common in nature. Plants defend wound sites to prevent pathogen invasion, but several pathogens can overcome spatial restriction and enter leaf tissues. The molecular mechanisms used by pathogens to suppress containment at wound infec...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Johana C Misas-Villamil, Izabella Kolodziejek, Emerson Crabill, Farnusch Kaschani, Sherry Niessen, Takayuki Shindo, Markus Kaiser, James R Alfano, Renier A L van der Hoorn
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/17e0158d36c04a6d98f9c38f88400e9d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:17e0158d36c04a6d98f9c38f88400e9d
record_format	dspace
spelling	oai:doaj.org-article:17e0158d36c04a6d98f9c38f88400e9d2021-11-18T06:05:50ZPseudomonas syringae pv. syringae uses proteasome inhibitor syringolin A to colonize from wound infection sites.1553-73661553-737410.1371/journal.ppat.1003281https://doaj.org/article/17e0158d36c04a6d98f9c38f88400e9d2013-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23555272/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Infection of plants by bacterial leaf pathogens at wound sites is common in nature. Plants defend wound sites to prevent pathogen invasion, but several pathogens can overcome spatial restriction and enter leaf tissues. The molecular mechanisms used by pathogens to suppress containment at wound infection sites are poorly understood. Here, we studied Pseudomonas syringae strains causing brown spot on bean and blossom blight on pear. These strains exist as epiphytes that can cause disease upon wounding caused by hail, sand storms and frost. We demonstrate that these strains overcome spatial restriction at wound sites by producing syringolin A (SylA), a small molecule proteasome inhibitor. Consequently, SylA-producing strains are able to escape from primary infection sites and colonize adjacent tissues along the vasculature. We found that SylA diffuses from the primary infection site and suppresses acquired resistance in adjacent tissues by blocking signaling by the stress hormone salicylic acid (SA). Thus, SylA diffusion creates a zone of SA-insensitive tissue that is prepared for subsequent colonization. In addition, SylA promotes bacterial motility and suppresses immune responses at the primary infection site. These local immune responses do not affect bacterial growth and were weak compared to effector-triggered immunity. Thus, SylA facilitates colonization from wounding sites by increasing bacterial motility and suppressing SA signaling in adjacent tissues.Johana C Misas-VillamilIzabella KolodziejekEmerson CrabillFarnusch KaschaniSherry NiessenTakayuki ShindoMarkus KaiserJames R AlfanoRenier A L van der HoornPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 3, p e1003281 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Johana C Misas-Villamil Izabella Kolodziejek Emerson Crabill Farnusch Kaschani Sherry Niessen Takayuki Shindo Markus Kaiser James R Alfano Renier A L van der Hoorn Pseudomonas syringae pv. syringae uses proteasome inhibitor syringolin A to colonize from wound infection sites.
description	Infection of plants by bacterial leaf pathogens at wound sites is common in nature. Plants defend wound sites to prevent pathogen invasion, but several pathogens can overcome spatial restriction and enter leaf tissues. The molecular mechanisms used by pathogens to suppress containment at wound infection sites are poorly understood. Here, we studied Pseudomonas syringae strains causing brown spot on bean and blossom blight on pear. These strains exist as epiphytes that can cause disease upon wounding caused by hail, sand storms and frost. We demonstrate that these strains overcome spatial restriction at wound sites by producing syringolin A (SylA), a small molecule proteasome inhibitor. Consequently, SylA-producing strains are able to escape from primary infection sites and colonize adjacent tissues along the vasculature. We found that SylA diffuses from the primary infection site and suppresses acquired resistance in adjacent tissues by blocking signaling by the stress hormone salicylic acid (SA). Thus, SylA diffusion creates a zone of SA-insensitive tissue that is prepared for subsequent colonization. In addition, SylA promotes bacterial motility and suppresses immune responses at the primary infection site. These local immune responses do not affect bacterial growth and were weak compared to effector-triggered immunity. Thus, SylA facilitates colonization from wounding sites by increasing bacterial motility and suppressing SA signaling in adjacent tissues.
format	article
author	Johana C Misas-Villamil Izabella Kolodziejek Emerson Crabill Farnusch Kaschani Sherry Niessen Takayuki Shindo Markus Kaiser James R Alfano Renier A L van der Hoorn
author_facet	Johana C Misas-Villamil Izabella Kolodziejek Emerson Crabill Farnusch Kaschani Sherry Niessen Takayuki Shindo Markus Kaiser James R Alfano Renier A L van der Hoorn
author_sort	Johana C Misas-Villamil
title	Pseudomonas syringae pv. syringae uses proteasome inhibitor syringolin A to colonize from wound infection sites.
title_short	Pseudomonas syringae pv. syringae uses proteasome inhibitor syringolin A to colonize from wound infection sites.
title_full	Pseudomonas syringae pv. syringae uses proteasome inhibitor syringolin A to colonize from wound infection sites.
title_fullStr	Pseudomonas syringae pv. syringae uses proteasome inhibitor syringolin A to colonize from wound infection sites.
title_full_unstemmed	Pseudomonas syringae pv. syringae uses proteasome inhibitor syringolin A to colonize from wound infection sites.
title_sort	pseudomonas syringae pv. syringae uses proteasome inhibitor syringolin a to colonize from wound infection sites.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/17e0158d36c04a6d98f9c38f88400e9d
work_keys_str_mv	AT johanacmisasvillamil pseudomonassyringaepvsyringaeusesproteasomeinhibitorsyringolinatocolonizefromwoundinfectionsites AT izabellakolodziejek pseudomonassyringaepvsyringaeusesproteasomeinhibitorsyringolinatocolonizefromwoundinfectionsites AT emersoncrabill pseudomonassyringaepvsyringaeusesproteasomeinhibitorsyringolinatocolonizefromwoundinfectionsites AT farnuschkaschani pseudomonassyringaepvsyringaeusesproteasomeinhibitorsyringolinatocolonizefromwoundinfectionsites AT sherryniessen pseudomonassyringaepvsyringaeusesproteasomeinhibitorsyringolinatocolonizefromwoundinfectionsites AT takayukishindo pseudomonassyringaepvsyringaeusesproteasomeinhibitorsyringolinatocolonizefromwoundinfectionsites AT markuskaiser pseudomonassyringaepvsyringaeusesproteasomeinhibitorsyringolinatocolonizefromwoundinfectionsites AT jamesralfano pseudomonassyringaepvsyringaeusesproteasomeinhibitorsyringolinatocolonizefromwoundinfectionsites AT renieralvanderhoorn pseudomonassyringaepvsyringaeusesproteasomeinhibitorsyringolinatocolonizefromwoundinfectionsites
_version_	1718424587265376256

Pseudomonas syringae pv. syringae uses proteasome inhibitor syringolin A to colonize from wound infection sites.

Ejemplares similares