Host-pathogen interactions of clinical S. aureus isolates to induce infective endocarditis

To evaluate potential pathomechanisms in the induction of infective endocarditis (IE), 34 Staphylococcus aureus (S. aureus) isolates, collected from patients with S. aureus endocarditis and from healthy individuals were investigated both in vitro and in vivo. S. aureus isolates were tested in vitro...

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Autores principales:	Christian Schwarz, Yasemin Töre, Vanessa Hoesker, Sabine Ameling, Katja Grün, Uwe Völker, P. Christian Schulze, Marcus Franz, Cornelius Faber, Frieder Schaumburg, Silke Niemann, Verena Hoerr
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	endocarditis clinical isolates mri staphylococcus aureus pathomechanisms Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/17e07ae6e55f46c3b05b93e01510bbfa
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spelling	oai:doaj.org-article:17e07ae6e55f46c3b05b93e01510bbfa2021-11-11T14:23:42ZHost-pathogen interactions of clinical S. aureus isolates to induce infective endocarditis2150-55942150-560810.1080/21505594.2021.1960107https://doaj.org/article/17e07ae6e55f46c3b05b93e01510bbfa2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2021.1960107https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608To evaluate potential pathomechanisms in the induction of infective endocarditis (IE), 34 Staphylococcus aureus (S. aureus) isolates, collected from patients with S. aureus endocarditis and from healthy individuals were investigated both in vitro and in vivo. S. aureus isolates were tested in vitro for their cytotoxicity, invasion and the association with platelets. Virulence factor expression profiles and cellular response were additionally investigated and tested for correlation with the ability of S. aureus to induce vegetations on the aortic valves in vivo. In an animal model of IE valvular conspicuity was assessed by in vivo magnetic resonance imaging at 9.4 T, histology and enrichment gene expression analysis. All S. aureus isolates tested in vivo caused a reliable infection and inflammation of the aortic valves, but could not be differentiated and categorized according to the measured in vitro virulence profiles and cytotoxicity. Results from in vitro assays did not correlate with the severity of IE. However, the isolates differed substantially in the activation and inhibition of pathways connected to the extracellular matrix and inflammatory response. Thus, comprehensive approaches of host-pathogen interactions and corresponding immune pathways are needed for the evaluation of the pathogenic capacity of bacteria. An improved understanding of the interaction between virulence factors and immune response in S. aureus infective endocarditis would offer novel possibilities for the development of therapeutic strategies and specific diagnostic imaging markers.Christian SchwarzYasemin TöreVanessa HoeskerSabine AmelingKatja GrünUwe VölkerP. Christian SchulzeMarcus FranzCornelius FaberFrieder SchaumburgSilke NiemannVerena HoerrTaylor & Francis Grouparticleendocarditisclinical isolatesmristaphylococcus aureuspathomechanismsInfectious and parasitic diseasesRC109-216ENVirulence, Vol 12, Iss 1, Pp 2073-2087 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	endocarditis clinical isolates mri staphylococcus aureus pathomechanisms Infectious and parasitic diseases RC109-216
spellingShingle	endocarditis clinical isolates mri staphylococcus aureus pathomechanisms Infectious and parasitic diseases RC109-216 Christian Schwarz Yasemin Töre Vanessa Hoesker Sabine Ameling Katja Grün Uwe Völker P. Christian Schulze Marcus Franz Cornelius Faber Frieder Schaumburg Silke Niemann Verena Hoerr Host-pathogen interactions of clinical S. aureus isolates to induce infective endocarditis
description	To evaluate potential pathomechanisms in the induction of infective endocarditis (IE), 34 Staphylococcus aureus (S. aureus) isolates, collected from patients with S. aureus endocarditis and from healthy individuals were investigated both in vitro and in vivo. S. aureus isolates were tested in vitro for their cytotoxicity, invasion and the association with platelets. Virulence factor expression profiles and cellular response were additionally investigated and tested for correlation with the ability of S. aureus to induce vegetations on the aortic valves in vivo. In an animal model of IE valvular conspicuity was assessed by in vivo magnetic resonance imaging at 9.4 T, histology and enrichment gene expression analysis. All S. aureus isolates tested in vivo caused a reliable infection and inflammation of the aortic valves, but could not be differentiated and categorized according to the measured in vitro virulence profiles and cytotoxicity. Results from in vitro assays did not correlate with the severity of IE. However, the isolates differed substantially in the activation and inhibition of pathways connected to the extracellular matrix and inflammatory response. Thus, comprehensive approaches of host-pathogen interactions and corresponding immune pathways are needed for the evaluation of the pathogenic capacity of bacteria. An improved understanding of the interaction between virulence factors and immune response in S. aureus infective endocarditis would offer novel possibilities for the development of therapeutic strategies and specific diagnostic imaging markers.
format	article
author	Christian Schwarz Yasemin Töre Vanessa Hoesker Sabine Ameling Katja Grün Uwe Völker P. Christian Schulze Marcus Franz Cornelius Faber Frieder Schaumburg Silke Niemann Verena Hoerr
author_facet	Christian Schwarz Yasemin Töre Vanessa Hoesker Sabine Ameling Katja Grün Uwe Völker P. Christian Schulze Marcus Franz Cornelius Faber Frieder Schaumburg Silke Niemann Verena Hoerr
author_sort	Christian Schwarz
title	Host-pathogen interactions of clinical S. aureus isolates to induce infective endocarditis
title_short	Host-pathogen interactions of clinical S. aureus isolates to induce infective endocarditis
title_full	Host-pathogen interactions of clinical S. aureus isolates to induce infective endocarditis
title_fullStr	Host-pathogen interactions of clinical S. aureus isolates to induce infective endocarditis
title_full_unstemmed	Host-pathogen interactions of clinical S. aureus isolates to induce infective endocarditis
title_sort	host-pathogen interactions of clinical s. aureus isolates to induce infective endocarditis
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/17e07ae6e55f46c3b05b93e01510bbfa
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Host-pathogen interactions of clinical S. aureus isolates to induce infective endocarditis

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