Quantifying the efficiency of Hydroxyapatite Mineralising Peptides

Abstract We present a non-destructive analytical calibration tool to allow quantitative assessment of individual calcium phosphates such as hydroxyapatite (HAP) from mixtures including brushite. Many experimental approaches are used to evaluate the mineralising capabilities of biomolecules including...

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Autores principales:	Robyn Plowright, David J. Belton, David L. Kaplan, Carole C. Perry
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/17e3fc5403d54507a659756aed5305ec
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spelling	oai:doaj.org-article:17e3fc5403d54507a659756aed5305ec2021-12-02T12:31:48ZQuantifying the efficiency of Hydroxyapatite Mineralising Peptides10.1038/s41598-017-07247-z2045-2322https://doaj.org/article/17e3fc5403d54507a659756aed5305ec2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07247-zhttps://doaj.org/toc/2045-2322Abstract We present a non-destructive analytical calibration tool to allow quantitative assessment of individual calcium phosphates such as hydroxyapatite (HAP) from mixtures including brushite. Many experimental approaches are used to evaluate the mineralising capabilities of biomolecules including peptides. However, it is difficult to quantitatively compare the efficacy of peptides in the promotion of mineralisation when inseparable mixtures of different minerals are produced. To address this challenge, a series of hydroxyapatite and brushite mixtures were produced as a percent/weight (0–100%) from pure components and multiple (N = 10) XRD patterns were collected for each mixture. A linear relationship between the ratio of selected peak heights and the molar ratio was found. Using this method, the mineralising capabilities of three known hydroxyapatite binding peptides, CaP(S) STLPIPHEFSRE, CaP(V) VTKHLNQISQSY and CaP(H) SVSVGMKPSPRP, was compared. All three directed mineralisation towards hydroxyapatite in a peptide concentration dependent manner. CaP(V) was most effective at inducing hydroxyapatite formation at higher reagent levels (Ca2+ = 200 mM), as also seen with peptide-silk chimeric materials, whereas CaP(S) was most effective when lower concentrations of calcium (20 mM) and phosphate were used. The approach can be extended to investigate HAP mineralisation in the presence of any number of mineralisation promoters or inhibitors.Robyn PlowrightDavid J. BeltonDavid L. KaplanCarole C. PerryNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Robyn Plowright David J. Belton David L. Kaplan Carole C. Perry Quantifying the efficiency of Hydroxyapatite Mineralising Peptides
description	Abstract We present a non-destructive analytical calibration tool to allow quantitative assessment of individual calcium phosphates such as hydroxyapatite (HAP) from mixtures including brushite. Many experimental approaches are used to evaluate the mineralising capabilities of biomolecules including peptides. However, it is difficult to quantitatively compare the efficacy of peptides in the promotion of mineralisation when inseparable mixtures of different minerals are produced. To address this challenge, a series of hydroxyapatite and brushite mixtures were produced as a percent/weight (0–100%) from pure components and multiple (N = 10) XRD patterns were collected for each mixture. A linear relationship between the ratio of selected peak heights and the molar ratio was found. Using this method, the mineralising capabilities of three known hydroxyapatite binding peptides, CaP(S) STLPIPHEFSRE, CaP(V) VTKHLNQISQSY and CaP(H) SVSVGMKPSPRP, was compared. All three directed mineralisation towards hydroxyapatite in a peptide concentration dependent manner. CaP(V) was most effective at inducing hydroxyapatite formation at higher reagent levels (Ca2+ = 200 mM), as also seen with peptide-silk chimeric materials, whereas CaP(S) was most effective when lower concentrations of calcium (20 mM) and phosphate were used. The approach can be extended to investigate HAP mineralisation in the presence of any number of mineralisation promoters or inhibitors.
format	article
author	Robyn Plowright David J. Belton David L. Kaplan Carole C. Perry
author_facet	Robyn Plowright David J. Belton David L. Kaplan Carole C. Perry
author_sort	Robyn Plowright
title	Quantifying the efficiency of Hydroxyapatite Mineralising Peptides
title_short	Quantifying the efficiency of Hydroxyapatite Mineralising Peptides
title_full	Quantifying the efficiency of Hydroxyapatite Mineralising Peptides
title_fullStr	Quantifying the efficiency of Hydroxyapatite Mineralising Peptides
title_full_unstemmed	Quantifying the efficiency of Hydroxyapatite Mineralising Peptides
title_sort	quantifying the efficiency of hydroxyapatite mineralising peptides
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/17e3fc5403d54507a659756aed5305ec
work_keys_str_mv	AT robynplowright quantifyingtheefficiencyofhydroxyapatitemineralisingpeptides AT davidjbelton quantifyingtheefficiencyofhydroxyapatitemineralisingpeptides AT davidlkaplan quantifyingtheefficiencyofhydroxyapatitemineralisingpeptides AT carolecperry quantifyingtheefficiencyofhydroxyapatitemineralisingpeptides
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Quantifying the efficiency of Hydroxyapatite Mineralising Peptides

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