Micropatterning biomineralization with immobilized mother of pearl proteins

Abstract In response to the drawbacks of autograft donor-site morbidity and bone morphogenetic protein type 2 (BMP2) carcinogenesis and ectopic bone formation, there has been an increased research focus towards developing alternatives capable of achieving spatial control over bone formation. Here we...

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Autores principales:	Kristopher A. White, Vincent J. Cali, Ronke M. Olabisi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/17e4d14ea51c48f0b7445c7b0c12acef
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spelling	oai:doaj.org-article:17e4d14ea51c48f0b7445c7b0c12acef2021-12-02T13:57:25ZMicropatterning biomineralization with immobilized mother of pearl proteins10.1038/s41598-021-81534-82045-2322https://doaj.org/article/17e4d14ea51c48f0b7445c7b0c12acef2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81534-8https://doaj.org/toc/2045-2322Abstract In response to the drawbacks of autograft donor-site morbidity and bone morphogenetic protein type 2 (BMP2) carcinogenesis and ectopic bone formation, there has been an increased research focus towards developing alternatives capable of achieving spatial control over bone formation. Here we show for the first time both osteogenic differentiation and mineralization (from solution or mediated by cells) occurring within predetermined microscopic patterns. Our results revealed that both PEGylated BMP2 and nacre proteins induced stem cell osteodifferentiation in microscopic patterns when these proteins were covalently bonded in patterns onto polyethylene glycol diacrylate (PEGDA) hydrogel substrates; however, only nacre proteins induced mineralization localized to the micropatterns. These findings have broad implications on the design and development of orthopedic biomaterials and drug delivery.Kristopher A. WhiteVincent J. CaliRonke M. OlabisiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kristopher A. White Vincent J. Cali Ronke M. Olabisi Micropatterning biomineralization with immobilized mother of pearl proteins
description	Abstract In response to the drawbacks of autograft donor-site morbidity and bone morphogenetic protein type 2 (BMP2) carcinogenesis and ectopic bone formation, there has been an increased research focus towards developing alternatives capable of achieving spatial control over bone formation. Here we show for the first time both osteogenic differentiation and mineralization (from solution or mediated by cells) occurring within predetermined microscopic patterns. Our results revealed that both PEGylated BMP2 and nacre proteins induced stem cell osteodifferentiation in microscopic patterns when these proteins were covalently bonded in patterns onto polyethylene glycol diacrylate (PEGDA) hydrogel substrates; however, only nacre proteins induced mineralization localized to the micropatterns. These findings have broad implications on the design and development of orthopedic biomaterials and drug delivery.
format	article
author	Kristopher A. White Vincent J. Cali Ronke M. Olabisi
author_facet	Kristopher A. White Vincent J. Cali Ronke M. Olabisi
author_sort	Kristopher A. White
title	Micropatterning biomineralization with immobilized mother of pearl proteins
title_short	Micropatterning biomineralization with immobilized mother of pearl proteins
title_full	Micropatterning biomineralization with immobilized mother of pearl proteins
title_fullStr	Micropatterning biomineralization with immobilized mother of pearl proteins
title_full_unstemmed	Micropatterning biomineralization with immobilized mother of pearl proteins
title_sort	micropatterning biomineralization with immobilized mother of pearl proteins
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/17e4d14ea51c48f0b7445c7b0c12acef
work_keys_str_mv	AT kristopherawhite micropatterningbiomineralizationwithimmobilizedmotherofpearlproteins AT vincentjcali micropatterningbiomineralizationwithimmobilizedmotherofpearlproteins AT ronkemolabisi micropatterningbiomineralizationwithimmobilizedmotherofpearlproteins
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Micropatterning biomineralization with immobilized mother of pearl proteins

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