Increased expression of IRE1α and stress-related signal transduction proteins in ischemia-reperfusion injured retina
Natsuyo Hata1, Toshiyuki Oshitari1,2, Akiko Yokoyama1,3, Yoshinori Mitamura1, Shuichi Yamamoto11Department of Ophthalmology and Visual Science, Chiba University Graduate School of Medicine, Chuo-ku, Chiba, Japan; 2Department of Ophthalmology, Kimitsu Central Hospital, Kisarazu City, Chiba, Japan; 3D...
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Dove Medical Press
2008
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oai:doaj.org-article:17f06ec573454e408d48e3b73ea664062021-12-02T00:55:47ZIncreased expression of IRE1α and stress-related signal transduction proteins in ischemia-reperfusion injured retina1177-54671177-5483https://doaj.org/article/17f06ec573454e408d48e3b73ea664062008-08-01T00:00:00Zhttp://www.dovepress.com/increased-expression-of-ire1alpha-and-stress-related-signal-transducti-a1967https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Natsuyo Hata1, Toshiyuki Oshitari1,2, Akiko Yokoyama1,3, Yoshinori Mitamura1, Shuichi Yamamoto11Department of Ophthalmology and Visual Science, Chiba University Graduate School of Medicine, Chuo-ku, Chiba, Japan; 2Department of Ophthalmology, Kimitsu Central Hospital, Kisarazu City, Chiba, Japan; 3Department of Ophthalmology, Inoue Memorial Hospital, Chuo-ku, Chiba, JapanAbstract: The purpose of this study was to determine whether the expression of ER stress-related factors IRE1α, apoptosis signal-regulating kinase 1 (ASK1), SAPK/ERK kinase 1 (SEK1) and c-Jun N-terminal kinase (JNK) is associated with the damaged retinal neurons induced by ischemia-reperfusion injury. After 60 minutes of ischemia, the rat retinas were reperfused, and retinas were isolated and fixed after 6, 9, 12, 18, and 24 hours, and 2, 5, and 9 days of reperfusion. Cryosections were immunostained with Fluoro-Jade B, a degenerating neuron marker to label degenerating neurons. Semi-quantitative analysis of the expression of IRE1α, ASK1, SEK1, and JNK were performed in both control and ischemic retinas. In ischemic retinas, the intensities of IRE1α immunoreactivity in the ganglion cell layer (GCL) were significantly higher than in the control retinas. In ischemic retinas, the numbers of SEK1-, ASK1-, and JNK-positive cells were significantly increased in the GCL compared to those in the control retinas. In addition, the cells that were positive for SEK1-, ASK1-, and JNK were also positive for Fluoro-Jade B-positive cells. These results indicate that the increased expression of ER stress-related factors was, in part, associated with the retinal neuronal abnormalities after ischemia-reperfusion injury in rat retinas.Keywords: endoplasmic reticulum, IRE1α, apoptosis signal-regulating kinase 1, SAPK/ERK kinase 1, c-Jun N-terminal kinase, Fluoro-Jade B, ischemia-reperfusion injury Natsuyo HataToshiyuki OshitariAkiko YokoyamaYoshinori MitamuraShuichi YamamotoDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2008, Iss Issue 4, Pp 743-752 (2008) |
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Ophthalmology RE1-994 |
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Ophthalmology RE1-994 Natsuyo Hata Toshiyuki Oshitari Akiko Yokoyama Yoshinori Mitamura Shuichi Yamamoto Increased expression of IRE1α and stress-related signal transduction proteins in ischemia-reperfusion injured retina |
| description |
Natsuyo Hata1, Toshiyuki Oshitari1,2, Akiko Yokoyama1,3, Yoshinori Mitamura1, Shuichi Yamamoto11Department of Ophthalmology and Visual Science, Chiba University Graduate School of Medicine, Chuo-ku, Chiba, Japan; 2Department of Ophthalmology, Kimitsu Central Hospital, Kisarazu City, Chiba, Japan; 3Department of Ophthalmology, Inoue Memorial Hospital, Chuo-ku, Chiba, JapanAbstract: The purpose of this study was to determine whether the expression of ER stress-related factors IRE1α, apoptosis signal-regulating kinase 1 (ASK1), SAPK/ERK kinase 1 (SEK1) and c-Jun N-terminal kinase (JNK) is associated with the damaged retinal neurons induced by ischemia-reperfusion injury. After 60 minutes of ischemia, the rat retinas were reperfused, and retinas were isolated and fixed after 6, 9, 12, 18, and 24 hours, and 2, 5, and 9 days of reperfusion. Cryosections were immunostained with Fluoro-Jade B, a degenerating neuron marker to label degenerating neurons. Semi-quantitative analysis of the expression of IRE1α, ASK1, SEK1, and JNK were performed in both control and ischemic retinas. In ischemic retinas, the intensities of IRE1α immunoreactivity in the ganglion cell layer (GCL) were significantly higher than in the control retinas. In ischemic retinas, the numbers of SEK1-, ASK1-, and JNK-positive cells were significantly increased in the GCL compared to those in the control retinas. In addition, the cells that were positive for SEK1-, ASK1-, and JNK were also positive for Fluoro-Jade B-positive cells. These results indicate that the increased expression of ER stress-related factors was, in part, associated with the retinal neuronal abnormalities after ischemia-reperfusion injury in rat retinas.Keywords: endoplasmic reticulum, IRE1α, apoptosis signal-regulating kinase 1, SAPK/ERK kinase 1, c-Jun N-terminal kinase, Fluoro-Jade B, ischemia-reperfusion injury |
| format |
article |
| author |
Natsuyo Hata Toshiyuki Oshitari Akiko Yokoyama Yoshinori Mitamura Shuichi Yamamoto |
| author_facet |
Natsuyo Hata Toshiyuki Oshitari Akiko Yokoyama Yoshinori Mitamura Shuichi Yamamoto |
| author_sort |
Natsuyo Hata |
| title |
Increased expression of IRE1α and stress-related signal transduction proteins in ischemia-reperfusion injured retina |
| title_short |
Increased expression of IRE1α and stress-related signal transduction proteins in ischemia-reperfusion injured retina |
| title_full |
Increased expression of IRE1α and stress-related signal transduction proteins in ischemia-reperfusion injured retina |
| title_fullStr |
Increased expression of IRE1α and stress-related signal transduction proteins in ischemia-reperfusion injured retina |
| title_full_unstemmed |
Increased expression of IRE1α and stress-related signal transduction proteins in ischemia-reperfusion injured retina |
| title_sort |
increased expression of ire1α and stress-related signal transduction proteins in ischemia-reperfusion injured retina |
| publisher |
Dove Medical Press |
| publishDate |
2008 |
| url |
https://doaj.org/article/17f06ec573454e408d48e3b73ea66406 |
| work_keys_str_mv |
AT natsuyohata increasedexpressionofire1ampalphaandstressrelatedsignaltransductionproteinsinischemiareperfusioninjuredretina AT toshiyukioshitari increasedexpressionofire1ampalphaandstressrelatedsignaltransductionproteinsinischemiareperfusioninjuredretina AT akikoyokoyama increasedexpressionofire1ampalphaandstressrelatedsignaltransductionproteinsinischemiareperfusioninjuredretina AT yoshinorimitamura increasedexpressionofire1ampalphaandstressrelatedsignaltransductionproteinsinischemiareperfusioninjuredretina AT shuichiyamamoto increasedexpressionofire1ampalphaandstressrelatedsignaltransductionproteinsinischemiareperfusioninjuredretina |
| _version_ |
1718403365947310080 |