A Boron Delivery Antibody (BDA) with Boronated Specific Residues: New Perspectives in Boron Neutron Capture Therapy from an In Silico Investigation

Boron Neutron Capture Therapy (BNCT) is a tumor cell-selective radiotherapy based on a nuclear reaction that occurs when the isotope boron-10 (<sup>10</sup>B) is radiated by low-energy thermal neutrons or epithermal neutrons, triggering a nuclear fission response and enabling a selective...

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Autores principales: Alessandro Rondina, Paola Fossa, Alessandro Orro, Luciano Milanesi, Antonella De Palma, Davide Perico, Pier Luigi Mauri, Pasqualina D’Ursi
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Publicado: MDPI AG 2021
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spelling oai:doaj.org-article:17f18813770e4ea0bfacae64a0c261052021-11-25T17:13:05ZA Boron Delivery Antibody (BDA) with Boronated Specific Residues: New Perspectives in Boron Neutron Capture Therapy from an In Silico Investigation10.3390/cells101132252073-4409https://doaj.org/article/17f18813770e4ea0bfacae64a0c261052021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3225https://doaj.org/toc/2073-4409Boron Neutron Capture Therapy (BNCT) is a tumor cell-selective radiotherapy based on a nuclear reaction that occurs when the isotope boron-10 (<sup>10</sup>B) is radiated by low-energy thermal neutrons or epithermal neutrons, triggering a nuclear fission response and enabling a selective administration of irradiation to cells. Hence, we need to create novel delivery agents containing <sup>10</sup>B with high tumor selectivity, but also exhibiting low intrinsic toxicity, fast clearance from normal tissue and blood, and no pharmaceutical effects. In the past, boronated monoclonal antibodies have been proposed using large boron-containing molecules or dendrimers, but with no investigations in relation to maintaining antibody specificity and structural and functional features. This work aims at improving the potential of monoclonal antibodies applied to BNCT therapy, identifying in silico the best native residues suitable to be substituted with a boronated one, carefully evaluating the effect of boronation on the 3D structure of the monoclonal antibody and on its binding affinity. A boronated monoclonal antibody was thus generated for specific <sup>10</sup>B delivery. In this context, we have developed a case study of Boron Delivery Antibody Identification Pipeline, which has been tested on cetuximab. Cetuximab is an epidermal growth factor receptor (EGFR) inhibitor used in the treatment of metastatic colorectal cancer, metastatic non-small cell lung cancer, and head and neck cancer.Alessandro RondinaPaola FossaAlessandro OrroLuciano MilanesiAntonella De PalmaDavide PericoPier Luigi MauriPasqualina D’UrsiMDPI AGarticleBoron Neutron Capture Therapy4-borono-L-phenylalanineBoron Delivery Antibody strategydockingmolecular dynamicsBiology (General)QH301-705.5ENCells, Vol 10, Iss 3225, p 3225 (2021)
institution DOAJ
collection DOAJ
language EN
topic Boron Neutron Capture Therapy
4-borono-L-phenylalanine
Boron Delivery Antibody strategy
docking
molecular dynamics
Biology (General)
QH301-705.5
spellingShingle Boron Neutron Capture Therapy
4-borono-L-phenylalanine
Boron Delivery Antibody strategy
docking
molecular dynamics
Biology (General)
QH301-705.5
Alessandro Rondina
Paola Fossa
Alessandro Orro
Luciano Milanesi
Antonella De Palma
Davide Perico
Pier Luigi Mauri
Pasqualina D’Ursi
A Boron Delivery Antibody (BDA) with Boronated Specific Residues: New Perspectives in Boron Neutron Capture Therapy from an In Silico Investigation
description Boron Neutron Capture Therapy (BNCT) is a tumor cell-selective radiotherapy based on a nuclear reaction that occurs when the isotope boron-10 (<sup>10</sup>B) is radiated by low-energy thermal neutrons or epithermal neutrons, triggering a nuclear fission response and enabling a selective administration of irradiation to cells. Hence, we need to create novel delivery agents containing <sup>10</sup>B with high tumor selectivity, but also exhibiting low intrinsic toxicity, fast clearance from normal tissue and blood, and no pharmaceutical effects. In the past, boronated monoclonal antibodies have been proposed using large boron-containing molecules or dendrimers, but with no investigations in relation to maintaining antibody specificity and structural and functional features. This work aims at improving the potential of monoclonal antibodies applied to BNCT therapy, identifying in silico the best native residues suitable to be substituted with a boronated one, carefully evaluating the effect of boronation on the 3D structure of the monoclonal antibody and on its binding affinity. A boronated monoclonal antibody was thus generated for specific <sup>10</sup>B delivery. In this context, we have developed a case study of Boron Delivery Antibody Identification Pipeline, which has been tested on cetuximab. Cetuximab is an epidermal growth factor receptor (EGFR) inhibitor used in the treatment of metastatic colorectal cancer, metastatic non-small cell lung cancer, and head and neck cancer.
format article
author Alessandro Rondina
Paola Fossa
Alessandro Orro
Luciano Milanesi
Antonella De Palma
Davide Perico
Pier Luigi Mauri
Pasqualina D’Ursi
author_facet Alessandro Rondina
Paola Fossa
Alessandro Orro
Luciano Milanesi
Antonella De Palma
Davide Perico
Pier Luigi Mauri
Pasqualina D’Ursi
author_sort Alessandro Rondina
title A Boron Delivery Antibody (BDA) with Boronated Specific Residues: New Perspectives in Boron Neutron Capture Therapy from an In Silico Investigation
title_short A Boron Delivery Antibody (BDA) with Boronated Specific Residues: New Perspectives in Boron Neutron Capture Therapy from an In Silico Investigation
title_full A Boron Delivery Antibody (BDA) with Boronated Specific Residues: New Perspectives in Boron Neutron Capture Therapy from an In Silico Investigation
title_fullStr A Boron Delivery Antibody (BDA) with Boronated Specific Residues: New Perspectives in Boron Neutron Capture Therapy from an In Silico Investigation
title_full_unstemmed A Boron Delivery Antibody (BDA) with Boronated Specific Residues: New Perspectives in Boron Neutron Capture Therapy from an In Silico Investigation
title_sort boron delivery antibody (bda) with boronated specific residues: new perspectives in boron neutron capture therapy from an in silico investigation
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/17f18813770e4ea0bfacae64a0c26105
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