Expression analysis of selected genes involved in tryptophan metabolic pathways in Egyptian children with Autism Spectrum Disorder and learning disabilities

Abstract Autism Spectrum Disorder (ASD) and learning disabilities are neurodevelopmental disabilities characterized by dramatically increasing incidence rates, yet the exact etiology for these disabilities is not identified. Impairment in tryptophan metabolism has been suggested to participate in th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Aliaa M. Higazi, Hanan M. Kamel, Emad A. Abdel-Naeem, Noha M. Abdullah, Doaa M. Mahrous, Ashraf M. Osman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/17f51597f9004c6ca731f220fe35572a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:17f51597f9004c6ca731f220fe35572a
record_format	dspace
spelling	oai:doaj.org-article:17f51597f9004c6ca731f220fe35572a2021-12-02T16:36:12ZExpression analysis of selected genes involved in tryptophan metabolic pathways in Egyptian children with Autism Spectrum Disorder and learning disabilities10.1038/s41598-021-86162-w2045-2322https://doaj.org/article/17f51597f9004c6ca731f220fe35572a2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86162-whttps://doaj.org/toc/2045-2322Abstract Autism Spectrum Disorder (ASD) and learning disabilities are neurodevelopmental disabilities characterized by dramatically increasing incidence rates, yet the exact etiology for these disabilities is not identified. Impairment in tryptophan metabolism has been suggested to participate in the pathogenesis of ASD, however, further validation of its involvement is required. Additionally, its role in learning disabilities is still uninvestigated. Our objective was to evaluate some aspects of tryptophan metabolism in ASD children (N = 45) compared to children with learning disabilities (N = 44) and healthy controls (N = 40) by measuring the expression levels of the MAOA, HAAO and AADAT genes using real-time RT-qPCR. We also aimed to correlate the expression patterns of these genes with parental ages at the time of childbirth, levels of serum iron, and vitamin D3 and zinc/copper ratio, as possible risk factors for ASD. Results demonstrated a significant decrease in the expression of the selected genes within ASD children (p < 0.001) relative to children with learning disabilities and healthy controls, which significantly associated with the levels of our targeted risk factors (p < 0.05) and negatively correlated to ASD scoring (p < 0.001). In conclusion, this study suggests that the expression of the MAOA, HAAO and AADAT genes may underpin the pathophysiology of ASD.Aliaa M. HigaziHanan M. KamelEmad A. Abdel-NaeemNoha M. AbdullahDoaa M. MahrousAshraf M. OsmanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aliaa M. Higazi Hanan M. Kamel Emad A. Abdel-Naeem Noha M. Abdullah Doaa M. Mahrous Ashraf M. Osman Expression analysis of selected genes involved in tryptophan metabolic pathways in Egyptian children with Autism Spectrum Disorder and learning disabilities
description	Abstract Autism Spectrum Disorder (ASD) and learning disabilities are neurodevelopmental disabilities characterized by dramatically increasing incidence rates, yet the exact etiology for these disabilities is not identified. Impairment in tryptophan metabolism has been suggested to participate in the pathogenesis of ASD, however, further validation of its involvement is required. Additionally, its role in learning disabilities is still uninvestigated. Our objective was to evaluate some aspects of tryptophan metabolism in ASD children (N = 45) compared to children with learning disabilities (N = 44) and healthy controls (N = 40) by measuring the expression levels of the MAOA, HAAO and AADAT genes using real-time RT-qPCR. We also aimed to correlate the expression patterns of these genes with parental ages at the time of childbirth, levels of serum iron, and vitamin D3 and zinc/copper ratio, as possible risk factors for ASD. Results demonstrated a significant decrease in the expression of the selected genes within ASD children (p < 0.001) relative to children with learning disabilities and healthy controls, which significantly associated with the levels of our targeted risk factors (p < 0.05) and negatively correlated to ASD scoring (p < 0.001). In conclusion, this study suggests that the expression of the MAOA, HAAO and AADAT genes may underpin the pathophysiology of ASD.
format	article
author	Aliaa M. Higazi Hanan M. Kamel Emad A. Abdel-Naeem Noha M. Abdullah Doaa M. Mahrous Ashraf M. Osman
author_facet	Aliaa M. Higazi Hanan M. Kamel Emad A. Abdel-Naeem Noha M. Abdullah Doaa M. Mahrous Ashraf M. Osman
author_sort	Aliaa M. Higazi
title	Expression analysis of selected genes involved in tryptophan metabolic pathways in Egyptian children with Autism Spectrum Disorder and learning disabilities
title_short	Expression analysis of selected genes involved in tryptophan metabolic pathways in Egyptian children with Autism Spectrum Disorder and learning disabilities
title_full	Expression analysis of selected genes involved in tryptophan metabolic pathways in Egyptian children with Autism Spectrum Disorder and learning disabilities
title_fullStr	Expression analysis of selected genes involved in tryptophan metabolic pathways in Egyptian children with Autism Spectrum Disorder and learning disabilities
title_full_unstemmed	Expression analysis of selected genes involved in tryptophan metabolic pathways in Egyptian children with Autism Spectrum Disorder and learning disabilities
title_sort	expression analysis of selected genes involved in tryptophan metabolic pathways in egyptian children with autism spectrum disorder and learning disabilities
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/17f51597f9004c6ca731f220fe35572a
work_keys_str_mv	AT aliaamhigazi expressionanalysisofselectedgenesinvolvedintryptophanmetabolicpathwaysinegyptianchildrenwithautismspectrumdisorderandlearningdisabilities AT hananmkamel expressionanalysisofselectedgenesinvolvedintryptophanmetabolicpathwaysinegyptianchildrenwithautismspectrumdisorderandlearningdisabilities AT emadaabdelnaeem expressionanalysisofselectedgenesinvolvedintryptophanmetabolicpathwaysinegyptianchildrenwithautismspectrumdisorderandlearningdisabilities AT nohamabdullah expressionanalysisofselectedgenesinvolvedintryptophanmetabolicpathwaysinegyptianchildrenwithautismspectrumdisorderandlearningdisabilities AT doaammahrous expressionanalysisofselectedgenesinvolvedintryptophanmetabolicpathwaysinegyptianchildrenwithautismspectrumdisorderandlearningdisabilities AT ashrafmosman expressionanalysisofselectedgenesinvolvedintryptophanmetabolicpathwaysinegyptianchildrenwithautismspectrumdisorderandlearningdisabilities
_version_	1718383617466433536

Expression analysis of selected genes involved in tryptophan metabolic pathways in Egyptian children with Autism Spectrum Disorder and learning disabilities

Ejemplares similares