Investigation of silver nanoparticles on titanium surface created by ion implantation technology

István Lampé,1 Dezső Beke,2 Sándor Biri,3 István Csarnovics,4 Attila Csik,3 Zsuzsanna Dombrádi,5 Péter Hajdu,3 Viktória Hegedűs,6 Richárd Rácz,3 István Varga,7 Csaba Hegedűs11Department of B...

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Autores principales:	Lampé I, Beke D, Biri S, Csarnovics I, Csik A, Dombrádi Z, Hajdu P, Hegedűs V, Rácz R, Varga I, Hegedűs C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Implants Peri-implant inflammation Silver Nanoparticles Antibacterial effect Titanium Geometrical model Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/17f6f322dd4a486c9706c6d641034129
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spelling	oai:doaj.org-article:17f6f322dd4a486c9706c6d6410341292021-12-02T06:23:55ZInvestigation of silver nanoparticles on titanium surface created by ion implantation technology1178-2013https://doaj.org/article/17f6f322dd4a486c9706c6d6410341292019-07-01T00:00:00Zhttps://www.dovepress.com/investigation-of-silver-nanoparticles-on-titanium-surface-created-by-i-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013István Lampé,1 Dezső Beke,2 Sándor Biri,3 István Csarnovics,4 Attila Csik,3 Zsuzsanna Dombrádi,5 Péter Hajdu,3 Viktória Hegedűs,6 Richárd Rácz,3 István Varga,7 Csaba Hegedűs11Department of Biomaterials and Prosthetic Dentistry, Faculty of Dentistry, University of Debrecen, Debrecen, Hungary; 2Department of Solid State Physics, University of Debrecen, Debrecen, Hungary; 3Hungarian Academy of Sciences, Institute for Nuclear Research, Debrecen, Hungary; 4Department of Experimental Physics, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary; 5Department of Medical Microbiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary; 6Department of Orthodontics, Faculty of Dentistry, University of Debrecen, Debrecen, Hungary; 7Department of Periodontology, Faculty of Dentistry, University of Debrecen, Debrecen, HungaryObjectives: Using dental Ti implants has become a well-accepted and used method for replacing missing dentition. It has become evident that in many cases peri-implant inflammation develops. The objective was to create and evaluate the antibacterial effect of silver nanoparticle (Ag-NP) coated Ti surfaces that can help to prevent such processes if applied on the surface of dental implants.Methods: Annealing I, Ag ion implantation by the beam of an Electron Cyclotron Resonance Ion Source (ECRIS), Ag Physical Vapor Deposition (PVD), Annealing II procedures were used, respectively, to create a safely anchored Ag-NP layer on 1x1 cm2, Grade 2 titanium samples. The antibacterial effect was evaluated by culturing Staphylococcus aureus (ATCC 29213) on the surfaces of the samples for 8 hours, and comparing the results to that of glass as control and of pure titanium samples. Alamar Blue assay was carried out to check cytotoxicity.Results: It was proved that silver nanoparticles were present on the treated surfaces. The average diameter of the particles was 58 nm, with a 25 nm deviation and Gaussian distribution, the the filling factor was 25%. Antibacterial evaluation revealed that the nanoparticle covered samples had an antibacterial effect of 64.6% that was statistically significant. Tests also proved that the nanoparticles are safely anchored to the titanium surface and are not cytotoxic.Conclusion: Creating a silver nanoparticle layer can be an option to add antibacterial features to the implant surface and to help in the prevention of peri-implant inflammatory processes. Recent studies demonstrated that silver nanoparticles can induce pathology in mammal cells, thus safe fixation of the particles is essential to prevent them from getting into the circulation.Keywords: implants, peri-implant inflammation, oxyde layer, cytocompatibility, antibacterial effect, physical vapor deposition, geometrical modelLampé IBeke DBiri SCsarnovics ICsik ADombrádi ZHajdu PHegedűs VRácz RVarga IHegedűs CDove Medical PressarticleImplantsPeri-implant inflammationSilverNanoparticlesAntibacterial effectTitaniumGeometrical modelMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 4709-4721 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Implants Peri-implant inflammation Silver Nanoparticles Antibacterial effect Titanium Geometrical model Medicine (General) R5-920
spellingShingle	Implants Peri-implant inflammation Silver Nanoparticles Antibacterial effect Titanium Geometrical model Medicine (General) R5-920 Lampé I Beke D Biri S Csarnovics I Csik A Dombrádi Z Hajdu P Hegedűs V Rácz R Varga I Hegedűs C Investigation of silver nanoparticles on titanium surface created by ion implantation technology
description	István Lampé,1 Dezső Beke,2 Sándor Biri,3 István Csarnovics,4 Attila Csik,3 Zsuzsanna Dombrádi,5 Péter Hajdu,3 Viktória Hegedűs,6 Richárd Rácz,3 István Varga,7 Csaba Hegedűs11Department of Biomaterials and Prosthetic Dentistry, Faculty of Dentistry, University of Debrecen, Debrecen, Hungary; 2Department of Solid State Physics, University of Debrecen, Debrecen, Hungary; 3Hungarian Academy of Sciences, Institute for Nuclear Research, Debrecen, Hungary; 4Department of Experimental Physics, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary; 5Department of Medical Microbiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary; 6Department of Orthodontics, Faculty of Dentistry, University of Debrecen, Debrecen, Hungary; 7Department of Periodontology, Faculty of Dentistry, University of Debrecen, Debrecen, HungaryObjectives: Using dental Ti implants has become a well-accepted and used method for replacing missing dentition. It has become evident that in many cases peri-implant inflammation develops. The objective was to create and evaluate the antibacterial effect of silver nanoparticle (Ag-NP) coated Ti surfaces that can help to prevent such processes if applied on the surface of dental implants.Methods: Annealing I, Ag ion implantation by the beam of an Electron Cyclotron Resonance Ion Source (ECRIS), Ag Physical Vapor Deposition (PVD), Annealing II procedures were used, respectively, to create a safely anchored Ag-NP layer on 1x1 cm2, Grade 2 titanium samples. The antibacterial effect was evaluated by culturing Staphylococcus aureus (ATCC 29213) on the surfaces of the samples for 8 hours, and comparing the results to that of glass as control and of pure titanium samples. Alamar Blue assay was carried out to check cytotoxicity.Results: It was proved that silver nanoparticles were present on the treated surfaces. The average diameter of the particles was 58 nm, with a 25 nm deviation and Gaussian distribution, the the filling factor was 25%. Antibacterial evaluation revealed that the nanoparticle covered samples had an antibacterial effect of 64.6% that was statistically significant. Tests also proved that the nanoparticles are safely anchored to the titanium surface and are not cytotoxic.Conclusion: Creating a silver nanoparticle layer can be an option to add antibacterial features to the implant surface and to help in the prevention of peri-implant inflammatory processes. Recent studies demonstrated that silver nanoparticles can induce pathology in mammal cells, thus safe fixation of the particles is essential to prevent them from getting into the circulation.Keywords: implants, peri-implant inflammation, oxyde layer, cytocompatibility, antibacterial effect, physical vapor deposition, geometrical model
format	article
author	Lampé I Beke D Biri S Csarnovics I Csik A Dombrádi Z Hajdu P Hegedűs V Rácz R Varga I Hegedűs C
author_facet	Lampé I Beke D Biri S Csarnovics I Csik A Dombrádi Z Hajdu P Hegedűs V Rácz R Varga I Hegedűs C
author_sort	Lampé I
title	Investigation of silver nanoparticles on titanium surface created by ion implantation technology
title_short	Investigation of silver nanoparticles on titanium surface created by ion implantation technology
title_full	Investigation of silver nanoparticles on titanium surface created by ion implantation technology
title_fullStr	Investigation of silver nanoparticles on titanium surface created by ion implantation technology
title_full_unstemmed	Investigation of silver nanoparticles on titanium surface created by ion implantation technology
title_sort	investigation of silver nanoparticles on titanium surface created by ion implantation technology
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/17f6f322dd4a486c9706c6d641034129
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Investigation of silver nanoparticles on titanium surface created by ion implantation technology

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