Association of the single nucleotide polymorphism C1858T of the PTPN22 gene with unexplained recurrent pregnancy loss: A case-control study

Abstract Background: Lymphoid-tyrosine-phosphatase which is encoded by the protein tyrosine phosphatase non-receptor 22 (PTPN22) gene plays a pivotal role in the regulation of immune responses by dephosphorylating several signaling intermediates of immune cells. Objective: Since a balanced immune re...

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Autores principales:	Fateme Khanbarari, Nasrin Ghasemi, Mahmood Vakili, Morteza Samadi
Formato:	article
Lenguaje:	EN
Publicado:	Shahid Sadoughi University of Medical Sciences 2021
Materias:	recurrent pregnancy loss, ptpn22 protein, single nucleotide polymorphism. Gynecology and obstetrics RG1-991 Reproduction QH471-489
Acceso en línea:	https://doaj.org/article/17f7f340d8e440ce87ec344fef0ecfd5
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spelling	oai:doaj.org-article:17f7f340d8e440ce87ec344fef0ecfd52021-11-16T07:24:40ZAssociation of the single nucleotide polymorphism C1858T of the PTPN22 gene with unexplained recurrent pregnancy loss: A case-control study2476-41082476-377210.18502/ijrm.v19i10.9819https://doaj.org/article/17f7f340d8e440ce87ec344fef0ecfd52021-10-01T00:00:00Zhttps://doi.org/10.18502/ijrm.v19i10.9819https://doaj.org/toc/2476-4108https://doaj.org/toc/2476-3772Abstract Background: Lymphoid-tyrosine-phosphatase which is encoded by the protein tyrosine phosphatase non-receptor 22 (PTPN22) gene plays a pivotal role in the regulation of immune responses by dephosphorylating several signaling intermediates of immune cells. Objective: Since a balanced immune response has been shown to be important during pregnancy, the purpose of this research was to compare the frequency of the PTPN22 C1858T polymorphism in women with unexplained recurrent pregnancy loss (URPL) vs. in a control group for the first time. Materials and Methods: Genomic DNA from 200 individuals with URPL and 200 individuals without URPL (the control group) at the infertility center in Yazd, Iran was isolated using the salting-out method. The PTPN22 C1858T polymorphism of the two groups was analyzed using polymerase chain reaction-restriction fragment length polymorphism. Genotype frequencies in the women with URPL and the fertile control group were compared using the Chi-square test. Results: There were significant differences in the frequency of the PTPN22 1858T polymorphism in the URPL individuals vs. the healthy controls, i.e. 32.0% and 21.5%, respectively (p = 0.01). Conclusion: Our findings suggest that the PTPN22 1858T polymorphism could play a role in recurrent pregnancy loss. Therefore, genotyping of the mentioned polymorphism can help clinicians to predict the probable risk of URPL.Fateme KhanbarariNasrin GhasemiMahmood VakiliMorteza SamadiShahid Sadoughi University of Medical Sciencesarticlerecurrent pregnancy loss, ptpn22 protein, single nucleotide polymorphism.Gynecology and obstetricsRG1-991ReproductionQH471-489ENInternational Journal of Reproductive BioMedicine, Vol 19, Iss 10, Pp 873-880 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	recurrent pregnancy loss, ptpn22 protein, single nucleotide polymorphism. Gynecology and obstetrics RG1-991 Reproduction QH471-489
spellingShingle	recurrent pregnancy loss, ptpn22 protein, single nucleotide polymorphism. Gynecology and obstetrics RG1-991 Reproduction QH471-489 Fateme Khanbarari Nasrin Ghasemi Mahmood Vakili Morteza Samadi Association of the single nucleotide polymorphism C1858T of the PTPN22 gene with unexplained recurrent pregnancy loss: A case-control study
description	Abstract Background: Lymphoid-tyrosine-phosphatase which is encoded by the protein tyrosine phosphatase non-receptor 22 (PTPN22) gene plays a pivotal role in the regulation of immune responses by dephosphorylating several signaling intermediates of immune cells. Objective: Since a balanced immune response has been shown to be important during pregnancy, the purpose of this research was to compare the frequency of the PTPN22 C1858T polymorphism in women with unexplained recurrent pregnancy loss (URPL) vs. in a control group for the first time. Materials and Methods: Genomic DNA from 200 individuals with URPL and 200 individuals without URPL (the control group) at the infertility center in Yazd, Iran was isolated using the salting-out method. The PTPN22 C1858T polymorphism of the two groups was analyzed using polymerase chain reaction-restriction fragment length polymorphism. Genotype frequencies in the women with URPL and the fertile control group were compared using the Chi-square test. Results: There were significant differences in the frequency of the PTPN22 1858T polymorphism in the URPL individuals vs. the healthy controls, i.e. 32.0% and 21.5%, respectively (p = 0.01). Conclusion: Our findings suggest that the PTPN22 1858T polymorphism could play a role in recurrent pregnancy loss. Therefore, genotyping of the mentioned polymorphism can help clinicians to predict the probable risk of URPL.
format	article
author	Fateme Khanbarari Nasrin Ghasemi Mahmood Vakili Morteza Samadi
author_facet	Fateme Khanbarari Nasrin Ghasemi Mahmood Vakili Morteza Samadi
author_sort	Fateme Khanbarari
title	Association of the single nucleotide polymorphism C1858T of the PTPN22 gene with unexplained recurrent pregnancy loss: A case-control study
title_short	Association of the single nucleotide polymorphism C1858T of the PTPN22 gene with unexplained recurrent pregnancy loss: A case-control study
title_full	Association of the single nucleotide polymorphism C1858T of the PTPN22 gene with unexplained recurrent pregnancy loss: A case-control study
title_fullStr	Association of the single nucleotide polymorphism C1858T of the PTPN22 gene with unexplained recurrent pregnancy loss: A case-control study
title_full_unstemmed	Association of the single nucleotide polymorphism C1858T of the PTPN22 gene with unexplained recurrent pregnancy loss: A case-control study
title_sort	association of the single nucleotide polymorphism c1858t of the ptpn22 gene with unexplained recurrent pregnancy loss: a case-control study
publisher	Shahid Sadoughi University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/17f7f340d8e440ce87ec344fef0ecfd5
work_keys_str_mv	AT fatemekhanbarari associationofthesinglenucleotidepolymorphismc1858toftheptpn22genewithunexplainedrecurrentpregnancylossacasecontrolstudy AT nasringhasemi associationofthesinglenucleotidepolymorphismc1858toftheptpn22genewithunexplainedrecurrentpregnancylossacasecontrolstudy AT mahmoodvakili associationofthesinglenucleotidepolymorphismc1858toftheptpn22genewithunexplainedrecurrentpregnancylossacasecontrolstudy AT mortezasamadi associationofthesinglenucleotidepolymorphismc1858toftheptpn22genewithunexplainedrecurrentpregnancylossacasecontrolstudy
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Association of the single nucleotide polymorphism C1858T of the PTPN22 gene with unexplained recurrent pregnancy loss: A case-control study

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