Tissue-specific activation of gene expression by the Synergistic Activation Mediator (SAM) CRISPRa system in mice
CRISPR-Cas9 is a gene editing tool that can be used to modulate gene expression. Here, the authors report the generation of a mouse model that express all components of the CRISPR-Cas9 guide directed Synergistic Activation Mediator (SAM), demonstrate that gene activation can be achieved with various...
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Nature Portfolio
2021
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oai:doaj.org-article:17fb14b41cbb4fa7890fbff2faf8ff192021-12-02T16:50:25ZTissue-specific activation of gene expression by the Synergistic Activation Mediator (SAM) CRISPRa system in mice10.1038/s41467-021-22932-42041-1723https://doaj.org/article/17fb14b41cbb4fa7890fbff2faf8ff192021-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-22932-4https://doaj.org/toc/2041-1723CRISPR-Cas9 is a gene editing tool that can be used to modulate gene expression. Here, the authors report the generation of a mouse model that express all components of the CRISPR-Cas9 guide directed Synergistic Activation Mediator (SAM), demonstrate that gene activation can be achieved with various delivery methods and include generation of a disease model of hypercholesterolemiaCharleen HuntSuzanne A. HartfordDerek WhiteEvangelos PefanisTimothy HannaClarissa HermanJarrell WileyHeather BrownQi SuYurong XinDennis VoroninHien NguyenJudith AltarejosKeith CrosbyJeffery HainesSarah CancelarichMeghan DrummondSven Moller-TankRyan MalpassJacqueline BuckleyMaria del Pilar Molina-PortelaGustavo DroguettDavid FrendeweyEric ChiaoBrian ZambrowiczGuochun GongNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-14 (2021) |
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DOAJ |
| language |
EN |
| topic |
Science Q |
| spellingShingle |
Science Q Charleen Hunt Suzanne A. Hartford Derek White Evangelos Pefanis Timothy Hanna Clarissa Herman Jarrell Wiley Heather Brown Qi Su Yurong Xin Dennis Voronin Hien Nguyen Judith Altarejos Keith Crosby Jeffery Haines Sarah Cancelarich Meghan Drummond Sven Moller-Tank Ryan Malpass Jacqueline Buckley Maria del Pilar Molina-Portela Gustavo Droguett David Frendewey Eric Chiao Brian Zambrowicz Guochun Gong Tissue-specific activation of gene expression by the Synergistic Activation Mediator (SAM) CRISPRa system in mice |
| description |
CRISPR-Cas9 is a gene editing tool that can be used to modulate gene expression. Here, the authors report the generation of a mouse model that express all components of the CRISPR-Cas9 guide directed Synergistic Activation Mediator (SAM), demonstrate that gene activation can be achieved with various delivery methods and include generation of a disease model of hypercholesterolemia |
| format |
article |
| author |
Charleen Hunt Suzanne A. Hartford Derek White Evangelos Pefanis Timothy Hanna Clarissa Herman Jarrell Wiley Heather Brown Qi Su Yurong Xin Dennis Voronin Hien Nguyen Judith Altarejos Keith Crosby Jeffery Haines Sarah Cancelarich Meghan Drummond Sven Moller-Tank Ryan Malpass Jacqueline Buckley Maria del Pilar Molina-Portela Gustavo Droguett David Frendewey Eric Chiao Brian Zambrowicz Guochun Gong |
| author_facet |
Charleen Hunt Suzanne A. Hartford Derek White Evangelos Pefanis Timothy Hanna Clarissa Herman Jarrell Wiley Heather Brown Qi Su Yurong Xin Dennis Voronin Hien Nguyen Judith Altarejos Keith Crosby Jeffery Haines Sarah Cancelarich Meghan Drummond Sven Moller-Tank Ryan Malpass Jacqueline Buckley Maria del Pilar Molina-Portela Gustavo Droguett David Frendewey Eric Chiao Brian Zambrowicz Guochun Gong |
| author_sort |
Charleen Hunt |
| title |
Tissue-specific activation of gene expression by the Synergistic Activation Mediator (SAM) CRISPRa system in mice |
| title_short |
Tissue-specific activation of gene expression by the Synergistic Activation Mediator (SAM) CRISPRa system in mice |
| title_full |
Tissue-specific activation of gene expression by the Synergistic Activation Mediator (SAM) CRISPRa system in mice |
| title_fullStr |
Tissue-specific activation of gene expression by the Synergistic Activation Mediator (SAM) CRISPRa system in mice |
| title_full_unstemmed |
Tissue-specific activation of gene expression by the Synergistic Activation Mediator (SAM) CRISPRa system in mice |
| title_sort |
tissue-specific activation of gene expression by the synergistic activation mediator (sam) crispra system in mice |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/17fb14b41cbb4fa7890fbff2faf8ff19 |
| work_keys_str_mv |
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1718383021897285632 |