Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi

Abstract Tomato wilt pathogen Fusarium oxysporum f. sp. lycopersici (Fol) is grouped into three races based on their pathogenicity to different host cultivars. Rapid detection and discrimination of Fol races in field soils is important to prevent tomato wilt disease. Although five types of point mut...

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Autores principales:	Yu Ayukawa, Saeri Hanyuda, Naoko Fujita, Ken Komatsu, Tsutomu Arie
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1806eb09bb9441adb2f618f8607f8004
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spelling	oai:doaj.org-article:1806eb09bb9441adb2f618f8607f80042021-12-02T12:32:06ZNovel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi10.1038/s41598-017-04084-y2045-2322https://doaj.org/article/1806eb09bb9441adb2f618f8607f80042017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04084-yhttps://doaj.org/toc/2045-2322Abstract Tomato wilt pathogen Fusarium oxysporum f. sp. lycopersici (Fol) is grouped into three races based on their pathogenicity to different host cultivars. Rapid detection and discrimination of Fol races in field soils is important to prevent tomato wilt disease. Although five types of point mutations in secreted in xylem 3 (SIX3) gene, which are characteristic of race 3, have been reported as a molecular marker for the race, detection of these point mutations is laborious. The aim of this study is to develop a rapid and accurate method for the detection of point mutations in SIX3 of Fol. Loop-mediated isothermal amplification (LAMP) of SIX3 gene with the universal QProbe as well as two joint DNAs followed by annealing curve analysis allowed us to specifically detect Fol and discriminate race 3 among other races in about one hour. Our developed method is applicable for detection of races of other plant pathogenic fungi as well as their pesticide-resistant mutants that arise through point mutations in a particular gene.Yu AyukawaSaeri HanyudaNaoko FujitaKen KomatsuTsutomu ArieNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yu Ayukawa Saeri Hanyuda Naoko Fujita Ken Komatsu Tsutomu Arie Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi
description	Abstract Tomato wilt pathogen Fusarium oxysporum f. sp. lycopersici (Fol) is grouped into three races based on their pathogenicity to different host cultivars. Rapid detection and discrimination of Fol races in field soils is important to prevent tomato wilt disease. Although five types of point mutations in secreted in xylem 3 (SIX3) gene, which are characteristic of race 3, have been reported as a molecular marker for the race, detection of these point mutations is laborious. The aim of this study is to develop a rapid and accurate method for the detection of point mutations in SIX3 of Fol. Loop-mediated isothermal amplification (LAMP) of SIX3 gene with the universal QProbe as well as two joint DNAs followed by annealing curve analysis allowed us to specifically detect Fol and discriminate race 3 among other races in about one hour. Our developed method is applicable for detection of races of other plant pathogenic fungi as well as their pesticide-resistant mutants that arise through point mutations in a particular gene.
format	article
author	Yu Ayukawa Saeri Hanyuda Naoko Fujita Ken Komatsu Tsutomu Arie
author_facet	Yu Ayukawa Saeri Hanyuda Naoko Fujita Ken Komatsu Tsutomu Arie
author_sort	Yu Ayukawa
title	Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi
title_short	Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi
title_full	Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi
title_fullStr	Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi
title_full_unstemmed	Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi
title_sort	novel loop-mediated isothermal amplification (lamp) assay with a universal qprobe can detect snps determining races in plant pathogenic fungi
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1806eb09bb9441adb2f618f8607f8004
work_keys_str_mv	AT yuayukawa novelloopmediatedisothermalamplificationlampassaywithauniversalqprobecandetectsnpsdeterminingracesinplantpathogenicfungi AT saerihanyuda novelloopmediatedisothermalamplificationlampassaywithauniversalqprobecandetectsnpsdeterminingracesinplantpathogenicfungi AT naokofujita novelloopmediatedisothermalamplificationlampassaywithauniversalqprobecandetectsnpsdeterminingracesinplantpathogenicfungi AT kenkomatsu novelloopmediatedisothermalamplificationlampassaywithauniversalqprobecandetectsnpsdeterminingracesinplantpathogenicfungi AT tsutomuarie novelloopmediatedisothermalamplificationlampassaywithauniversalqprobecandetectsnpsdeterminingracesinplantpathogenicfungi
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Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi

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