Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells.

<h4>Background & aims</h4>In preterm infants, exfoliated gastric epithelial cells can be retrieved from aspirates sampled through the naso-gastric feeding tube. Our aims were to determine (1) whether the recovery of exfoliated cells is feasible at any time from birth through the remo...

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Autores principales: Bertrand Kaeffer, Arnaud Legrand, Thomas Moyon, Anne Frondas-Chauty, Hélène Billard, Omar Guzman-Quevedo, Dominique Darmaun, Jean-Christophe Rozé
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Publicado: Public Library of Science (PLoS) 2011
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spelling oai:doaj.org-article:1808435f8bc2461eb8854ea41f48c4062021-11-18T07:36:21ZNon-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells.1932-620310.1371/journal.pone.0025562https://doaj.org/article/1808435f8bc2461eb8854ea41f48c4062011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22028779/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background & aims</h4>In preterm infants, exfoliated gastric epithelial cells can be retrieved from aspirates sampled through the naso-gastric feeding tube. Our aims were to determine (1) whether the recovery of exfoliated cells is feasible at any time from birth through the removal of the nasogastric tube, (2) whether they can be grown in culture in vitro, and (3) whether the physiological state of exfoliated cells expressing H+/K+ -ATPases reflects that of their counterparts remaining in situ at the surface of the gastric epithelium in neonatal rat pups.<h4>Methods</h4>In infants, gastric fluid aspirates were collected weekly after birth or every 3 hours over 24-h periods, and related to clinical parameters (Biocollection PROG/09/18). In rat pups submitted to a single fasting/refeeding cycle, we explored circadian exfoliation with the cellular counter-parts in the gland. All samples were analyzed by confocal imaging and Enzyme-Linked Immunosorbent Assay.<h4>Results</h4>Epithelial cells were identified by microscopy using membrane-bound anti-H+/K+ ATPases antibody, assessed for nucleus integrity, and the expression of selected proteins (autophagy, circadian clock). On 34 infants, the H+/K+-ATPase-positive cells were consistently found quiescent, regardless of gestational age and feeding schedule from day-5 of life to the day of removal of the naso-gastric tube. By logistic regression analysis, we did find a positive correlation between the intensity of exfoliation (cellular loss per sample) and the postnatal age (p<0.001). The H+/K+ ATPase-positive cells established in culture retained the expression of a biomarker of progenitor status (Pouf5F1-Oct4). In rat pups, the expression pattern of Survivin in H+/K+ ATPase-positive exfoliated cells paralleled that observed in cells remaining at the surface of the gastric gland.<h4>Conclusions</h4>Tracking parietal cells can improve clinical monitoring and understanding of the autophagic death via the phosphatidylinositol 3-kinase/Akt/survivin pathway.Bertrand KaefferArnaud LegrandThomas MoyonAnne Frondas-ChautyHélène BillardOmar Guzman-QuevedoDominique DarmaunJean-Christophe RozéPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e25562 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Bertrand Kaeffer
Arnaud Legrand
Thomas Moyon
Anne Frondas-Chauty
Hélène Billard
Omar Guzman-Quevedo
Dominique Darmaun
Jean-Christophe Rozé
Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells.
description <h4>Background & aims</h4>In preterm infants, exfoliated gastric epithelial cells can be retrieved from aspirates sampled through the naso-gastric feeding tube. Our aims were to determine (1) whether the recovery of exfoliated cells is feasible at any time from birth through the removal of the nasogastric tube, (2) whether they can be grown in culture in vitro, and (3) whether the physiological state of exfoliated cells expressing H+/K+ -ATPases reflects that of their counterparts remaining in situ at the surface of the gastric epithelium in neonatal rat pups.<h4>Methods</h4>In infants, gastric fluid aspirates were collected weekly after birth or every 3 hours over 24-h periods, and related to clinical parameters (Biocollection PROG/09/18). In rat pups submitted to a single fasting/refeeding cycle, we explored circadian exfoliation with the cellular counter-parts in the gland. All samples were analyzed by confocal imaging and Enzyme-Linked Immunosorbent Assay.<h4>Results</h4>Epithelial cells were identified by microscopy using membrane-bound anti-H+/K+ ATPases antibody, assessed for nucleus integrity, and the expression of selected proteins (autophagy, circadian clock). On 34 infants, the H+/K+-ATPase-positive cells were consistently found quiescent, regardless of gestational age and feeding schedule from day-5 of life to the day of removal of the naso-gastric tube. By logistic regression analysis, we did find a positive correlation between the intensity of exfoliation (cellular loss per sample) and the postnatal age (p<0.001). The H+/K+ ATPase-positive cells established in culture retained the expression of a biomarker of progenitor status (Pouf5F1-Oct4). In rat pups, the expression pattern of Survivin in H+/K+ ATPase-positive exfoliated cells paralleled that observed in cells remaining at the surface of the gastric gland.<h4>Conclusions</h4>Tracking parietal cells can improve clinical monitoring and understanding of the autophagic death via the phosphatidylinositol 3-kinase/Akt/survivin pathway.
format article
author Bertrand Kaeffer
Arnaud Legrand
Thomas Moyon
Anne Frondas-Chauty
Hélène Billard
Omar Guzman-Quevedo
Dominique Darmaun
Jean-Christophe Rozé
author_facet Bertrand Kaeffer
Arnaud Legrand
Thomas Moyon
Anne Frondas-Chauty
Hélène Billard
Omar Guzman-Quevedo
Dominique Darmaun
Jean-Christophe Rozé
author_sort Bertrand Kaeffer
title Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells.
title_short Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells.
title_full Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells.
title_fullStr Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells.
title_full_unstemmed Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells.
title_sort non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells.
publisher Public Library of Science (PLoS)
publishDate 2011
url https://doaj.org/article/1808435f8bc2461eb8854ea41f48c406
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