Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells.

<h4>Background & aims</h4>In preterm infants, exfoliated gastric epithelial cells can be retrieved from aspirates sampled through the naso-gastric feeding tube. Our aims were to determine (1) whether the recovery of exfoliated cells is feasible at any time from birth through the remo...

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Autores principales:	Bertrand Kaeffer, Arnaud Legrand, Thomas Moyon, Anne Frondas-Chauty, Hélène Billard, Omar Guzman-Quevedo, Dominique Darmaun, Jean-Christophe Rozé
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1808435f8bc2461eb8854ea41f48c406
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spelling	oai:doaj.org-article:1808435f8bc2461eb8854ea41f48c4062021-11-18T07:36:21ZNon-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells.1932-620310.1371/journal.pone.0025562https://doaj.org/article/1808435f8bc2461eb8854ea41f48c4062011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22028779/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background & aims</h4>In preterm infants, exfoliated gastric epithelial cells can be retrieved from aspirates sampled through the naso-gastric feeding tube. Our aims were to determine (1) whether the recovery of exfoliated cells is feasible at any time from birth through the removal of the nasogastric tube, (2) whether they can be grown in culture in vitro, and (3) whether the physiological state of exfoliated cells expressing H+/K+ -ATPases reflects that of their counterparts remaining in situ at the surface of the gastric epithelium in neonatal rat pups.<h4>Methods</h4>In infants, gastric fluid aspirates were collected weekly after birth or every 3 hours over 24-h periods, and related to clinical parameters (Biocollection PROG/09/18). In rat pups submitted to a single fasting/refeeding cycle, we explored circadian exfoliation with the cellular counter-parts in the gland. All samples were analyzed by confocal imaging and Enzyme-Linked Immunosorbent Assay.<h4>Results</h4>Epithelial cells were identified by microscopy using membrane-bound anti-H+/K+ ATPases antibody, assessed for nucleus integrity, and the expression of selected proteins (autophagy, circadian clock). On 34 infants, the H+/K+-ATPase-positive cells were consistently found quiescent, regardless of gestational age and feeding schedule from day-5 of life to the day of removal of the naso-gastric tube. By logistic regression analysis, we did find a positive correlation between the intensity of exfoliation (cellular loss per sample) and the postnatal age (p<0.001). The H+/K+ ATPase-positive cells established in culture retained the expression of a biomarker of progenitor status (Pouf5F1-Oct4). In rat pups, the expression pattern of Survivin in H+/K+ ATPase-positive exfoliated cells paralleled that observed in cells remaining at the surface of the gastric gland.<h4>Conclusions</h4>Tracking parietal cells can improve clinical monitoring and understanding of the autophagic death via the phosphatidylinositol 3-kinase/Akt/survivin pathway.Bertrand KaefferArnaud LegrandThomas MoyonAnne Frondas-ChautyHélène BillardOmar Guzman-QuevedoDominique DarmaunJean-Christophe RozéPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e25562 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bertrand Kaeffer Arnaud Legrand Thomas Moyon Anne Frondas-Chauty Hélène Billard Omar Guzman-Quevedo Dominique Darmaun Jean-Christophe Rozé Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells.
description	<h4>Background & aims</h4>In preterm infants, exfoliated gastric epithelial cells can be retrieved from aspirates sampled through the naso-gastric feeding tube. Our aims were to determine (1) whether the recovery of exfoliated cells is feasible at any time from birth through the removal of the nasogastric tube, (2) whether they can be grown in culture in vitro, and (3) whether the physiological state of exfoliated cells expressing H+/K+ -ATPases reflects that of their counterparts remaining in situ at the surface of the gastric epithelium in neonatal rat pups.<h4>Methods</h4>In infants, gastric fluid aspirates were collected weekly after birth or every 3 hours over 24-h periods, and related to clinical parameters (Biocollection PROG/09/18). In rat pups submitted to a single fasting/refeeding cycle, we explored circadian exfoliation with the cellular counter-parts in the gland. All samples were analyzed by confocal imaging and Enzyme-Linked Immunosorbent Assay.<h4>Results</h4>Epithelial cells were identified by microscopy using membrane-bound anti-H+/K+ ATPases antibody, assessed for nucleus integrity, and the expression of selected proteins (autophagy, circadian clock). On 34 infants, the H+/K+-ATPase-positive cells were consistently found quiescent, regardless of gestational age and feeding schedule from day-5 of life to the day of removal of the naso-gastric tube. By logistic regression analysis, we did find a positive correlation between the intensity of exfoliation (cellular loss per sample) and the postnatal age (p<0.001). The H+/K+ ATPase-positive cells established in culture retained the expression of a biomarker of progenitor status (Pouf5F1-Oct4). In rat pups, the expression pattern of Survivin in H+/K+ ATPase-positive exfoliated cells paralleled that observed in cells remaining at the surface of the gastric gland.<h4>Conclusions</h4>Tracking parietal cells can improve clinical monitoring and understanding of the autophagic death via the phosphatidylinositol 3-kinase/Akt/survivin pathway.
format	article
author	Bertrand Kaeffer Arnaud Legrand Thomas Moyon Anne Frondas-Chauty Hélène Billard Omar Guzman-Quevedo Dominique Darmaun Jean-Christophe Rozé
author_facet	Bertrand Kaeffer Arnaud Legrand Thomas Moyon Anne Frondas-Chauty Hélène Billard Omar Guzman-Quevedo Dominique Darmaun Jean-Christophe Rozé
author_sort	Bertrand Kaeffer
title	Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells.
title_short	Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells.
title_full	Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells.
title_fullStr	Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells.
title_full_unstemmed	Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells.
title_sort	non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/1808435f8bc2461eb8854ea41f48c406
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Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells.

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