Generation of Functional Cardiomyocytes from Human Gastric Fibroblast-Derived Induced Pluripotent Stem Cells

Coronary artery diseases are major problems of the world. Coronary artery disease patients frequently suffer from peptic ulcers when they receive aspirin treatment. For diagnostic and therapeutic purposes, the implementation of panendoscopy (PES) with biopsy is necessary. Some biopsy samples are was...

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Autores principales:	Chih-Hsien Wu, Hsuan-Hwai Lin, Yi-Ying Wu, Yi-Lin Chiu, Li-Yen Huang, Cheng-Chung Cheng, Chung-Chi Yang, Tsung-Neng Tsai
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	induced pluripotent stem cells human gastric fibroblast cells cardiomyocytes Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/181686f5b90347c28acd24153d298c55
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spelling	oai:doaj.org-article:181686f5b90347c28acd24153d298c552021-11-25T16:49:05ZGeneration of Functional Cardiomyocytes from Human Gastric Fibroblast-Derived Induced Pluripotent Stem Cells10.3390/biomedicines91115652227-9059https://doaj.org/article/181686f5b90347c28acd24153d298c552021-10-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1565https://doaj.org/toc/2227-9059Coronary artery diseases are major problems of the world. Coronary artery disease patients frequently suffer from peptic ulcers when they receive aspirin treatment. For diagnostic and therapeutic purposes, the implementation of panendoscopy (PES) with biopsy is necessary. Some biopsy samples are wasted after the assay is completed. In the present study, we established a protocol for human gastric fibroblast isolation and induced pluripotent stem cell (iPSC) generation from gastric fibroblasts via PES with biopsy. We showed that these iPSCs can be differentiated into functional cardiomyocytes in vitro. To our knowledge, this is the first study to generate iPSCs from gastric fibroblasts in vitro.Chih-Hsien WuHsuan-Hwai LinYi-Ying WuYi-Lin ChiuLi-Yen HuangCheng-Chung ChengChung-Chi YangTsung-Neng TsaiMDPI AGarticleinduced pluripotent stem cellshuman gastric fibroblast cellscardiomyocytesBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1565, p 1565 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	induced pluripotent stem cells human gastric fibroblast cells cardiomyocytes Biology (General) QH301-705.5
spellingShingle	induced pluripotent stem cells human gastric fibroblast cells cardiomyocytes Biology (General) QH301-705.5 Chih-Hsien Wu Hsuan-Hwai Lin Yi-Ying Wu Yi-Lin Chiu Li-Yen Huang Cheng-Chung Cheng Chung-Chi Yang Tsung-Neng Tsai Generation of Functional Cardiomyocytes from Human Gastric Fibroblast-Derived Induced Pluripotent Stem Cells
description	Coronary artery diseases are major problems of the world. Coronary artery disease patients frequently suffer from peptic ulcers when they receive aspirin treatment. For diagnostic and therapeutic purposes, the implementation of panendoscopy (PES) with biopsy is necessary. Some biopsy samples are wasted after the assay is completed. In the present study, we established a protocol for human gastric fibroblast isolation and induced pluripotent stem cell (iPSC) generation from gastric fibroblasts via PES with biopsy. We showed that these iPSCs can be differentiated into functional cardiomyocytes in vitro. To our knowledge, this is the first study to generate iPSCs from gastric fibroblasts in vitro.
format	article
author	Chih-Hsien Wu Hsuan-Hwai Lin Yi-Ying Wu Yi-Lin Chiu Li-Yen Huang Cheng-Chung Cheng Chung-Chi Yang Tsung-Neng Tsai
author_facet	Chih-Hsien Wu Hsuan-Hwai Lin Yi-Ying Wu Yi-Lin Chiu Li-Yen Huang Cheng-Chung Cheng Chung-Chi Yang Tsung-Neng Tsai
author_sort	Chih-Hsien Wu
title	Generation of Functional Cardiomyocytes from Human Gastric Fibroblast-Derived Induced Pluripotent Stem Cells
title_short	Generation of Functional Cardiomyocytes from Human Gastric Fibroblast-Derived Induced Pluripotent Stem Cells
title_full	Generation of Functional Cardiomyocytes from Human Gastric Fibroblast-Derived Induced Pluripotent Stem Cells
title_fullStr	Generation of Functional Cardiomyocytes from Human Gastric Fibroblast-Derived Induced Pluripotent Stem Cells
title_full_unstemmed	Generation of Functional Cardiomyocytes from Human Gastric Fibroblast-Derived Induced Pluripotent Stem Cells
title_sort	generation of functional cardiomyocytes from human gastric fibroblast-derived induced pluripotent stem cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/181686f5b90347c28acd24153d298c55
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Generation of Functional Cardiomyocytes from Human Gastric Fibroblast-Derived Induced Pluripotent Stem Cells

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