Refinement of 16S rRNA gene analysis for low biomass biospecimens
Abstract High-throughput phylogenetic 16S rRNA gene analysis has permitted to thoroughly delve into microbial community complexity and to understand host-microbiota interactions in health and disease. The analysis comprises sample collection and storage, genomic DNA extraction, 16S rRNA gene amplifi...
Saved in:
Main Authors: | Remy Villette, Gaelle Autaa, Sophie Hind, Johanna B. Holm, Alicia Moreno-Sabater, Martin Larsen |
---|---|
Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2021
|
Subjects: | |
Online Access: | https://doaj.org/article/1818d4b5788f4d449b131b0ed8fa2f39 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Controlling for Contaminants in Low-Biomass 16S rRNA Gene Sequencing Experiments
by: Lisa Karstens, et al.
Published: (2019) -
Critical Relevance of Stochastic Effects on Low-Bacterial-Biomass 16S rRNA Gene Analysis
by: John R. Erb-Downward, et al.
Published: (2020) -
Sputum microbiota in tuberculosis as revealed by 16S rRNA pyrosequencing.
by: Man Kit Cheung, et al.
Published: (2013) -
16S rRNA Amplicon Sequencing for Epidemiological Surveys of Bacteria in Wildlife
by: Maxime Galan, et al.
Published: (2016) -
16S rRNA/rRNA Gene Ratios and Cell Activity Staining Reveal Consistent Patterns of Microbial Activity in Plant-Associated Soil
by: Alan W. Bowsher, et al.
Published: (2019)