Transgenerational Diapause as an Avoidance Strategy against Bacterial Pathogens in <italic toggle="yes">Caenorhabditis elegans</italic>

ABSTRACT The dynamic response of organisms exposed to environmental pathogens determines their survival or demise, and the outcome of this interaction depends on the host’s susceptibility and pathogen-dependent virulence factors. The transmission of acquired information about the nature of a pathoge...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	M. Fernanda Palominos, Lidia Verdugo, Carolaing Gabaldon, Bernardo Pollak, Javiera Ortíz-Severín, Macarena A. Varas, Francisco P. Chávez, Andrea Calixto
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Caenorhabditis elegans RNA interference defense diapause pathogenesis survival strategies Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/181a43d820d049dab4e6a0203d210d15
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:181a43d820d049dab4e6a0203d210d15
record_format	dspace
spelling	oai:doaj.org-article:181a43d820d049dab4e6a0203d210d152021-11-15T15:51:51ZTransgenerational Diapause as an Avoidance Strategy against Bacterial Pathogens in <italic toggle="yes">Caenorhabditis elegans</italic>10.1128/mBio.01234-172150-7511https://doaj.org/article/181a43d820d049dab4e6a0203d210d152017-11-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01234-17https://doaj.org/toc/2150-7511ABSTRACT The dynamic response of organisms exposed to environmental pathogens determines their survival or demise, and the outcome of this interaction depends on the host’s susceptibility and pathogen-dependent virulence factors. The transmission of acquired information about the nature of a pathogen to progeny may ensure effective defensive strategies for the progeny’s survival in adverse environments. Environmental RNA interference (RNAi) is a systemic and heritable mechanism and has recently been linked to antibacterial and antifungal defenses in both plants and animals. Here, we report that the second generation of Caenorhabditis elegans living on pathogenic bacteria can avoid bacterial infection by entering diapause in an RNAi pathway-dependent mechanism. Furthermore, we demonstrate that the information encoding this survival strategy is transgenerationally transmitted to the progeny via the maternal germ line. IMPORTANCE Bacteria vastly influence physiology and behavior, and yet, the specific mechanisms by which they cause behavioral changes in hosts are not known. We use C. elegans as a host and the bacteria they eat to understand how microbes trigger a behavioral change that helps animals to survive. We found that animals faced with an infection for two generations could enter a hibernationlike state, arresting development by forming dauer larvae. Dauers have closed mouths and effectively avoid infection. Animals accumulate information that is transgenerationally transmitted to the next generations to form dauers. This work gives insight on how bacteria communicate in noncanonical ways with their hosts, resulting in long-lasting effects providing survival strategies to the community.M. Fernanda PalominosLidia VerdugoCarolaing GabaldonBernardo PollakJaviera Ortíz-SeverínMacarena A. VarasFrancisco P. ChávezAndrea CalixtoAmerican Society for MicrobiologyarticleCaenorhabditis elegansRNA interferencedefensediapausepathogenesissurvival strategiesMicrobiologyQR1-502ENmBio, Vol 8, Iss 5 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Caenorhabditis elegans RNA interference defense diapause pathogenesis survival strategies Microbiology QR1-502
spellingShingle	Caenorhabditis elegans RNA interference defense diapause pathogenesis survival strategies Microbiology QR1-502 M. Fernanda Palominos Lidia Verdugo Carolaing Gabaldon Bernardo Pollak Javiera Ortíz-Severín Macarena A. Varas Francisco P. Chávez Andrea Calixto Transgenerational Diapause as an Avoidance Strategy against Bacterial Pathogens in <italic toggle="yes">Caenorhabditis elegans</italic>
description	ABSTRACT The dynamic response of organisms exposed to environmental pathogens determines their survival or demise, and the outcome of this interaction depends on the host’s susceptibility and pathogen-dependent virulence factors. The transmission of acquired information about the nature of a pathogen to progeny may ensure effective defensive strategies for the progeny’s survival in adverse environments. Environmental RNA interference (RNAi) is a systemic and heritable mechanism and has recently been linked to antibacterial and antifungal defenses in both plants and animals. Here, we report that the second generation of Caenorhabditis elegans living on pathogenic bacteria can avoid bacterial infection by entering diapause in an RNAi pathway-dependent mechanism. Furthermore, we demonstrate that the information encoding this survival strategy is transgenerationally transmitted to the progeny via the maternal germ line. IMPORTANCE Bacteria vastly influence physiology and behavior, and yet, the specific mechanisms by which they cause behavioral changes in hosts are not known. We use C. elegans as a host and the bacteria they eat to understand how microbes trigger a behavioral change that helps animals to survive. We found that animals faced with an infection for two generations could enter a hibernationlike state, arresting development by forming dauer larvae. Dauers have closed mouths and effectively avoid infection. Animals accumulate information that is transgenerationally transmitted to the next generations to form dauers. This work gives insight on how bacteria communicate in noncanonical ways with their hosts, resulting in long-lasting effects providing survival strategies to the community.
format	article
author	M. Fernanda Palominos Lidia Verdugo Carolaing Gabaldon Bernardo Pollak Javiera Ortíz-Severín Macarena A. Varas Francisco P. Chávez Andrea Calixto
author_facet	M. Fernanda Palominos Lidia Verdugo Carolaing Gabaldon Bernardo Pollak Javiera Ortíz-Severín Macarena A. Varas Francisco P. Chávez Andrea Calixto
author_sort	M. Fernanda Palominos
title	Transgenerational Diapause as an Avoidance Strategy against Bacterial Pathogens in <italic toggle="yes">Caenorhabditis elegans</italic>
title_short	Transgenerational Diapause as an Avoidance Strategy against Bacterial Pathogens in <italic toggle="yes">Caenorhabditis elegans</italic>
title_full	Transgenerational Diapause as an Avoidance Strategy against Bacterial Pathogens in <italic toggle="yes">Caenorhabditis elegans</italic>
title_fullStr	Transgenerational Diapause as an Avoidance Strategy against Bacterial Pathogens in <italic toggle="yes">Caenorhabditis elegans</italic>
title_full_unstemmed	Transgenerational Diapause as an Avoidance Strategy against Bacterial Pathogens in <italic toggle="yes">Caenorhabditis elegans</italic>
title_sort	transgenerational diapause as an avoidance strategy against bacterial pathogens in <italic toggle="yes">caenorhabditis elegans</italic>
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/181a43d820d049dab4e6a0203d210d15
work_keys_str_mv	AT mfernandapalominos transgenerationaldiapauseasanavoidancestrategyagainstbacterialpathogensinitalictoggleyescaenorhabditiselegansitalic AT lidiaverdugo transgenerationaldiapauseasanavoidancestrategyagainstbacterialpathogensinitalictoggleyescaenorhabditiselegansitalic AT carolainggabaldon transgenerationaldiapauseasanavoidancestrategyagainstbacterialpathogensinitalictoggleyescaenorhabditiselegansitalic AT bernardopollak transgenerationaldiapauseasanavoidancestrategyagainstbacterialpathogensinitalictoggleyescaenorhabditiselegansitalic AT javieraortizseverin transgenerationaldiapauseasanavoidancestrategyagainstbacterialpathogensinitalictoggleyescaenorhabditiselegansitalic AT macarenaavaras transgenerationaldiapauseasanavoidancestrategyagainstbacterialpathogensinitalictoggleyescaenorhabditiselegansitalic AT franciscopchavez transgenerationaldiapauseasanavoidancestrategyagainstbacterialpathogensinitalictoggleyescaenorhabditiselegansitalic AT andreacalixto transgenerationaldiapauseasanavoidancestrategyagainstbacterialpathogensinitalictoggleyescaenorhabditiselegansitalic
_version_	1718427321167249408

Transgenerational Diapause as an Avoidance Strategy against Bacterial Pathogens in <italic toggle="yes">Caenorhabditis elegans</italic>

Ejemplares similares