A versatile system for USER cloning-based assembly of expression vectors for mammalian cell engineering.

A new versatile mammalian vector system for protein production, cell biology analyses, and cell factory engineering was developed. The vector system applies the ligation-free uracil-excision based technique--USER cloning--to rapidly construct mammalian expression vectors of multiple DNA fragments an...

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Autores principales: Anne Mathilde Lund, Helene Faustrup Kildegaard, Maja Borup Kjær Petersen, Julie Rank, Bjarne Gram Hansen, Mikael Rørdam Andersen, Uffe Hasbro Mortensen
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2014
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Acceso en línea:https://doaj.org/article/181d730b6705404b876e3f3957db1fb4
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