Fabrication and characterization of silk fibroin-derived curcumin nanoparticles for cancer therapy

Vishal Gupta1, Abraham Aseh1,3, Carmen N R&iacute;os1, Bharat B Aggarwal2, Anshu B Mathur11Department of Plastic Surgery; 2Department of Experimental Therapeutics, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA; 3School of Pharmacy, Texas Southern University, Houston,...

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Autores principales:	Vishal Gupta, Abraham Aseh, Carmen N R&iacute;os, Bharat B Aggarwal, et al.
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2009
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/18203f270b4d4e0e85dc6cc2b4d6f91b
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spelling	oai:doaj.org-article:18203f270b4d4e0e85dc6cc2b4d6f91b2021-12-02T02:31:36ZFabrication and characterization of silk fibroin-derived curcumin nanoparticles for cancer therapy1176-91141178-2013https://doaj.org/article/18203f270b4d4e0e85dc6cc2b4d6f91b2009-05-01T00:00:00Zhttp://www.dovepress.com/fabrication-and-characterization-of-silk-fibroin-derived-curcumin-nano-a3131https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Vishal Gupta1, Abraham Aseh1,3, Carmen N R&iacute;os1, Bharat B Aggarwal2, Anshu B Mathur11Department of Plastic Surgery; 2Department of Experimental Therapeutics, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA; 3School of Pharmacy, Texas Southern University, Houston, TX, USAAbstract: Biologically derived nanoparticles (&lt;100 nm) were fabricated for local and sustained therapeutic curcumin delivery to cancer cells. Silk fibroin (SF) and chitosan (CS) polymers were blended noncovalently to encapsulate curcumin in various proportions of SF and CS (75:25, 50:50, and 25:75 SF:CS) or pure SF at two concentrations (0.1% w/v and 10% w/v) using the devised capillary-microdot technique. Curcumin-polymer conjugates were frozen, lyophilized, crystallized, suspended in phosphate-buffered saline for characterization, and tested for efficacy against breast cancer cells. All nanoparticle formulations except 0.1% w/v 50:50 SFCS were less than 100 nm in size as determined with the transmission electron microscopy. The entrapment and release of curcumin over eight days was highest for SF-derived nanoparticles as compared to all SFCS blends. The uptake and efficacy of SF-coated curcumin was significantly higher (p &lt; 0.001) than SFCS-coated curcumin in both low and high Her2/neu expressing breast cancer cells. Interestingly, the uptake of curcumin was highest for the high Her2/neu expressing breast cancer cells when delivered with a 10% w/v SF coating as compared to other formulations. In conclusion, SF-derived curcumin nanoparticles show higher efficacy against breast cancer cells and have&nbsp;the potential to treat in vivo breast tumors by local, sustained, and long-term therapeutic delivery as a biodegradable system.Keywords: biodegradable, nanoparticles, curcumin, silk fibroin, breast cancer cells Vishal GuptaAbraham AsehCarmen N R&iacute;osBharat B Aggarwalet al.Dove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2009, Iss default, Pp 115-122 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Vishal Gupta Abraham Aseh Carmen N R&iacute;os Bharat B Aggarwal et al. Fabrication and characterization of silk fibroin-derived curcumin nanoparticles for cancer therapy
description	Vishal Gupta1, Abraham Aseh1,3, Carmen N R&iacute;os1, Bharat B Aggarwal2, Anshu B Mathur11Department of Plastic Surgery; 2Department of Experimental Therapeutics, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA; 3School of Pharmacy, Texas Southern University, Houston, TX, USAAbstract: Biologically derived nanoparticles (&lt;100 nm) were fabricated for local and sustained therapeutic curcumin delivery to cancer cells. Silk fibroin (SF) and chitosan (CS) polymers were blended noncovalently to encapsulate curcumin in various proportions of SF and CS (75:25, 50:50, and 25:75 SF:CS) or pure SF at two concentrations (0.1% w/v and 10% w/v) using the devised capillary-microdot technique. Curcumin-polymer conjugates were frozen, lyophilized, crystallized, suspended in phosphate-buffered saline for characterization, and tested for efficacy against breast cancer cells. All nanoparticle formulations except 0.1% w/v 50:50 SFCS were less than 100 nm in size as determined with the transmission electron microscopy. The entrapment and release of curcumin over eight days was highest for SF-derived nanoparticles as compared to all SFCS blends. The uptake and efficacy of SF-coated curcumin was significantly higher (p &lt; 0.001) than SFCS-coated curcumin in both low and high Her2/neu expressing breast cancer cells. Interestingly, the uptake of curcumin was highest for the high Her2/neu expressing breast cancer cells when delivered with a 10% w/v SF coating as compared to other formulations. In conclusion, SF-derived curcumin nanoparticles show higher efficacy against breast cancer cells and have&nbsp;the potential to treat in vivo breast tumors by local, sustained, and long-term therapeutic delivery as a biodegradable system.Keywords: biodegradable, nanoparticles, curcumin, silk fibroin, breast cancer cells
format	article
author	Vishal Gupta Abraham Aseh Carmen N R&iacute;os Bharat B Aggarwal et al.
author_facet	Vishal Gupta Abraham Aseh Carmen N R&iacute;os Bharat B Aggarwal et al.
author_sort	Vishal Gupta
title	Fabrication and characterization of silk fibroin-derived curcumin nanoparticles for cancer therapy
title_short	Fabrication and characterization of silk fibroin-derived curcumin nanoparticles for cancer therapy
title_full	Fabrication and characterization of silk fibroin-derived curcumin nanoparticles for cancer therapy
title_fullStr	Fabrication and characterization of silk fibroin-derived curcumin nanoparticles for cancer therapy
title_full_unstemmed	Fabrication and characterization of silk fibroin-derived curcumin nanoparticles for cancer therapy
title_sort	fabrication and characterization of silk fibroin-derived curcumin nanoparticles for cancer therapy
publisher	Dove Medical Press
publishDate	2009
url	https://doaj.org/article/18203f270b4d4e0e85dc6cc2b4d6f91b
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Fabrication and characterization of silk fibroin-derived curcumin nanoparticles for cancer therapy

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