Genomic Methylated Cytosine Level during the Dedifferentiation and Cellular Competence in Coffea arabica Lines: Insights about the Different In Vitro Responses

Coffea arabica genotypes present distinct responses in vitro, and somaclonal variation occurrence has been reported. Global cytosine methylation is one of the epigenetic mechanisms that influences the Coffea in vitro responses. We aimed to establish the indirect...

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Autores principales:	João Paulo de Morais Oliveira, Natália Arruda Sanglard, Adésio Ferreira, Wellington Ronildo Clarindo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	coffee epigenetic indirect somatic embryogenesis plant tissue culture ploidy level Plant ecology QK900-989
Acceso en línea:	https://doaj.org/article/182702af93f24554821fe2e2dcedacea
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spelling	oai:doaj.org-article:182702af93f24554821fe2e2dcedacea2021-11-25T17:38:23ZGenomic Methylated Cytosine Level during the Dedifferentiation and Cellular Competence in <i>Coffea arabica</i> Lines: Insights about the Different In Vitro Responses10.3390/f121115361999-4907https://doaj.org/article/182702af93f24554821fe2e2dcedacea2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4907/12/11/1536https://doaj.org/toc/1999-4907<i>Coffea arabica</i> genotypes present distinct responses in vitro, and somaclonal variation occurrence has been reported. Global cytosine methylation is one of the epigenetic mechanisms that influences the <i>Coffea</i> in vitro responses. We aimed to establish the indirect somatic embryogenesis in <i>C. arabica</i> ‘Catuaí Vermelho’, ‘Caturra’ and ‘Oeiras’, associate the distinct responses to the methylated cytosine genomic level, and check the ploidy stability. Leaf explants were cultured in callus induction and proliferation medium. The resulted calli were transferred to the regeneration medium, and the mature cotyledonary somatic embryos were transferred to the seedling medium. ‘Oeiras’ exhibited the highest number of responsive leaf explants, followed by ‘Caturra’ and ‘Catuaí Vermelho’. Global methylated cytosine level increased over time in the ‘Catuaí Vermelho’ and ‘Caturra’ friable calli, remaining constant in ‘Oeiras’. ‘Oeiras’ did not regenerate somatic embryos, while ‘Catuaí Vermelho’ exhibited the highest number. Somatic embryo regeneration was associated with the increase of the methylated cytosine level. However, the ‘Catuaí Vermelho’ embryogenic calli showed a lower methylated cytosine level than ‘Caturra’. Recovered plantlets exhibited the same 2C value and chromosome number to the explant donors. Therefore, cytosine hypermethylation occurred during <i>C. arabica</i> indirect somatic embryogenesis, influencing cell competence and somatic embryos regeneration.João Paulo de Morais OliveiraNatália Arruda SanglardAdésio FerreiraWellington Ronildo ClarindoMDPI AGarticlecoffeeepigeneticindirect somatic embryogenesisplant tissue cultureploidy levelPlant ecologyQK900-989ENForests, Vol 12, Iss 1536, p 1536 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	coffee epigenetic indirect somatic embryogenesis plant tissue culture ploidy level Plant ecology QK900-989
spellingShingle	coffee epigenetic indirect somatic embryogenesis plant tissue culture ploidy level Plant ecology QK900-989 João Paulo de Morais Oliveira Natália Arruda Sanglard Adésio Ferreira Wellington Ronildo Clarindo Genomic Methylated Cytosine Level during the Dedifferentiation and Cellular Competence in <i>Coffea arabica</i> Lines: Insights about the Different In Vitro Responses
description	<i>Coffea arabica</i> genotypes present distinct responses in vitro, and somaclonal variation occurrence has been reported. Global cytosine methylation is one of the epigenetic mechanisms that influences the <i>Coffea</i> in vitro responses. We aimed to establish the indirect somatic embryogenesis in <i>C. arabica</i> ‘Catuaí Vermelho’, ‘Caturra’ and ‘Oeiras’, associate the distinct responses to the methylated cytosine genomic level, and check the ploidy stability. Leaf explants were cultured in callus induction and proliferation medium. The resulted calli were transferred to the regeneration medium, and the mature cotyledonary somatic embryos were transferred to the seedling medium. ‘Oeiras’ exhibited the highest number of responsive leaf explants, followed by ‘Caturra’ and ‘Catuaí Vermelho’. Global methylated cytosine level increased over time in the ‘Catuaí Vermelho’ and ‘Caturra’ friable calli, remaining constant in ‘Oeiras’. ‘Oeiras’ did not regenerate somatic embryos, while ‘Catuaí Vermelho’ exhibited the highest number. Somatic embryo regeneration was associated with the increase of the methylated cytosine level. However, the ‘Catuaí Vermelho’ embryogenic calli showed a lower methylated cytosine level than ‘Caturra’. Recovered plantlets exhibited the same 2C value and chromosome number to the explant donors. Therefore, cytosine hypermethylation occurred during <i>C. arabica</i> indirect somatic embryogenesis, influencing cell competence and somatic embryos regeneration.
format	article
author	João Paulo de Morais Oliveira Natália Arruda Sanglard Adésio Ferreira Wellington Ronildo Clarindo
author_facet	João Paulo de Morais Oliveira Natália Arruda Sanglard Adésio Ferreira Wellington Ronildo Clarindo
author_sort	João Paulo de Morais Oliveira
title	Genomic Methylated Cytosine Level during the Dedifferentiation and Cellular Competence in <i>Coffea arabica</i> Lines: Insights about the Different In Vitro Responses
title_short	Genomic Methylated Cytosine Level during the Dedifferentiation and Cellular Competence in <i>Coffea arabica</i> Lines: Insights about the Different In Vitro Responses
title_full	Genomic Methylated Cytosine Level during the Dedifferentiation and Cellular Competence in <i>Coffea arabica</i> Lines: Insights about the Different In Vitro Responses
title_fullStr	Genomic Methylated Cytosine Level during the Dedifferentiation and Cellular Competence in <i>Coffea arabica</i> Lines: Insights about the Different In Vitro Responses
title_full_unstemmed	Genomic Methylated Cytosine Level during the Dedifferentiation and Cellular Competence in <i>Coffea arabica</i> Lines: Insights about the Different In Vitro Responses
title_sort	genomic methylated cytosine level during the dedifferentiation and cellular competence in <i>coffea arabica</i> lines: insights about the different in vitro responses
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/182702af93f24554821fe2e2dcedacea
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Genomic Methylated Cytosine Level during the Dedifferentiation and Cellular Competence in <i>Coffea arabica</i> Lines: Insights about the Different In Vitro Responses

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