Oral Intake of Semi-refined Carrageenan by Rats Affects Apoptosis of Lymphocytes

Introduction. The safety of generally recognized as safe food additives E407 and E407a is under rigorous debate. The aim of our research was to evaluate the effects of the orally administered food additive E407a (semi-refined carrageenan) on the viability of lymphocytes and their cell death modes. M...

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Autores principales:	Anton Tkachenko, Anatolii Onishchenko
Formato:	article
Lenguaje:	EN
Publicado:	Shiraz University of Medical Sciences 2020
Materias:	processed eucheuma seaweed annexin v 7-aminoactinomycin d flow cytometry Medicine R
Acceso en línea:	https://doaj.org/article/1828297a01b9456ea613994b547da6ce
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spelling	oai:doaj.org-article:1828297a01b9456ea613994b547da6ce2021-11-14T08:12:23ZOral Intake of Semi-refined Carrageenan by Rats Affects Apoptosis of Lymphocytes2783-243010.30476/acrr.2021.88624.1068https://doaj.org/article/1828297a01b9456ea613994b547da6ce2020-12-01T00:00:00Zhttps://colorectalresearch.sums.ac.ir/article_47233_fd0dd1c35f47149821e8907ced99cb93.pdfhttps://doaj.org/toc/2783-2430Introduction. The safety of generally recognized as safe food additives E407 and E407a is under rigorous debate. The aim of our research was to evaluate the effects of the orally administered food additive E407a (semi-refined carrageenan) on the viability of lymphocytes and their cell death modes. Methods. Totally, 16 adult WAG rats were divided into two equal groups (experimental – oral intake of 140 mg/kg of E407a during 2 weeks; control – oral consumption of drinking water instead). Blood samples were used to obtain leukocyte suspensions stained with Annexin V-FITC and 7-aminoactinomycin D (7-AAD). The region of lymphocytes was analyzed after collecting data using a BD FACSCanto™ II flow cytometer. Results. Oral administration of E407a led to a decrease in the amount of viable (Annexin V-, 7-AAD-) circulating lymphocytes. Furthermore, exposure to semi-refined carrageenan resulted in an elevated number of early apoptotic (Annexin V+, 7-AAD-) lymphocytes. Their percentage was approximately 4 times higher in rats exposed to E407a compared with the control group. Conclusion. Our findings indicate that dietary intake of E407a promotes apoptosis of circulating lymphocytes. Keywords: processed Eucheuma seaweed, annexin V, 7-aminoactinomycin D, flow cytometry.Anton TkachenkoAnatolii OnishchenkoShiraz University of Medical Sciencesarticleprocessed eucheuma seaweedannexin v7-aminoactinomycin dflow cytometryMedicineRENIranian Journal of Colorectal Research, Vol 8, Iss 4, Pp 170-174 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	processed eucheuma seaweed annexin v 7-aminoactinomycin d flow cytometry Medicine R
spellingShingle	processed eucheuma seaweed annexin v 7-aminoactinomycin d flow cytometry Medicine R Anton Tkachenko Anatolii Onishchenko Oral Intake of Semi-refined Carrageenan by Rats Affects Apoptosis of Lymphocytes
description	Introduction. The safety of generally recognized as safe food additives E407 and E407a is under rigorous debate. The aim of our research was to evaluate the effects of the orally administered food additive E407a (semi-refined carrageenan) on the viability of lymphocytes and their cell death modes. Methods. Totally, 16 adult WAG rats were divided into two equal groups (experimental – oral intake of 140 mg/kg of E407a during 2 weeks; control – oral consumption of drinking water instead). Blood samples were used to obtain leukocyte suspensions stained with Annexin V-FITC and 7-aminoactinomycin D (7-AAD). The region of lymphocytes was analyzed after collecting data using a BD FACSCanto™ II flow cytometer. Results. Oral administration of E407a led to a decrease in the amount of viable (Annexin V-, 7-AAD-) circulating lymphocytes. Furthermore, exposure to semi-refined carrageenan resulted in an elevated number of early apoptotic (Annexin V+, 7-AAD-) lymphocytes. Their percentage was approximately 4 times higher in rats exposed to E407a compared with the control group. Conclusion. Our findings indicate that dietary intake of E407a promotes apoptosis of circulating lymphocytes. Keywords: processed Eucheuma seaweed, annexin V, 7-aminoactinomycin D, flow cytometry.
format	article
author	Anton Tkachenko Anatolii Onishchenko
author_facet	Anton Tkachenko Anatolii Onishchenko
author_sort	Anton Tkachenko
title	Oral Intake of Semi-refined Carrageenan by Rats Affects Apoptosis of Lymphocytes
title_short	Oral Intake of Semi-refined Carrageenan by Rats Affects Apoptosis of Lymphocytes
title_full	Oral Intake of Semi-refined Carrageenan by Rats Affects Apoptosis of Lymphocytes
title_fullStr	Oral Intake of Semi-refined Carrageenan by Rats Affects Apoptosis of Lymphocytes
title_full_unstemmed	Oral Intake of Semi-refined Carrageenan by Rats Affects Apoptosis of Lymphocytes
title_sort	oral intake of semi-refined carrageenan by rats affects apoptosis of lymphocytes
publisher	Shiraz University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/1828297a01b9456ea613994b547da6ce
work_keys_str_mv	AT antontkachenko oralintakeofsemirefinedcarrageenanbyratsaffectsapoptosisoflymphocytes AT anatoliionishchenko oralintakeofsemirefinedcarrageenanbyratsaffectsapoptosisoflymphocytes
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Oral Intake of Semi-refined Carrageenan by Rats Affects Apoptosis of Lymphocytes

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