Comprehensive Analysis of microRNAs in Human Adult Erythropoiesis

MicroRNAs (miRNAs) are small non-coding RNAs, which play an important role in various cellular and developmental processes. The study of miRNAs in erythropoiesis is crucial to uncover the cellular pathways that are modulated during the different stages of erythroid differentiation. Using erythroid c...

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Autores principales:	Aneesha Nath, Janakiram Rayabaram, Smitha Ijee, Abhirup Bagchi, Anurag Dutta Chaudhury, Debanjan Roy, Karthik Chambayil, Jyoti Singh, Yukio Nakamura, Shaji R. Velayudhan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	microRNAs small RNA sequencing erythropoiesis CD34+ HSPCs CRISPR-Cas9 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1833210d1d9f49d296cdd7d654be51af
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spelling	oai:doaj.org-article:1833210d1d9f49d296cdd7d654be51af2021-11-25T17:10:26ZComprehensive Analysis of microRNAs in Human Adult Erythropoiesis10.3390/cells101130182073-4409https://doaj.org/article/1833210d1d9f49d296cdd7d654be51af2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3018https://doaj.org/toc/2073-4409MicroRNAs (miRNAs) are small non-coding RNAs, which play an important role in various cellular and developmental processes. The study of miRNAs in erythropoiesis is crucial to uncover the cellular pathways that are modulated during the different stages of erythroid differentiation. Using erythroid cells derived from human CD34+ hematopoietic stem and progenitor cells (HSPCs)and small RNA sequencing, our study unravels the various miRNAs involved in critical cellular pathways in erythroid maturation. We analyzed the occupancy of erythroid transcription factors and chromatin accessibility in the promoter and enhancer regions of the differentially expressed miRNAs to integrate miRNAs in the transcriptional circuitry of erythropoiesis. Analysis of the targets of the differentially expressed miRNAs revealed novel pathways in erythroid differentiation. Finally, we described the application of Clustered regularly interspaced short palindromic repeats-Cas9 (CRISPR-Cas9) based editing of miRNAs to study their function in human erythropoiesis.Aneesha NathJanakiram RayabaramSmitha IjeeAbhirup BagchiAnurag Dutta ChaudhuryDebanjan RoyKarthik ChambayilJyoti SinghYukio NakamuraShaji R. VelayudhanMDPI AGarticlemicroRNAssmall RNA sequencingerythropoiesisCD34+ HSPCsCRISPR-Cas9Biology (General)QH301-705.5ENCells, Vol 10, Iss 3018, p 3018 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microRNAs small RNA sequencing erythropoiesis CD34+ HSPCs CRISPR-Cas9 Biology (General) QH301-705.5
spellingShingle	microRNAs small RNA sequencing erythropoiesis CD34+ HSPCs CRISPR-Cas9 Biology (General) QH301-705.5 Aneesha Nath Janakiram Rayabaram Smitha Ijee Abhirup Bagchi Anurag Dutta Chaudhury Debanjan Roy Karthik Chambayil Jyoti Singh Yukio Nakamura Shaji R. Velayudhan Comprehensive Analysis of microRNAs in Human Adult Erythropoiesis
description	MicroRNAs (miRNAs) are small non-coding RNAs, which play an important role in various cellular and developmental processes. The study of miRNAs in erythropoiesis is crucial to uncover the cellular pathways that are modulated during the different stages of erythroid differentiation. Using erythroid cells derived from human CD34+ hematopoietic stem and progenitor cells (HSPCs)and small RNA sequencing, our study unravels the various miRNAs involved in critical cellular pathways in erythroid maturation. We analyzed the occupancy of erythroid transcription factors and chromatin accessibility in the promoter and enhancer regions of the differentially expressed miRNAs to integrate miRNAs in the transcriptional circuitry of erythropoiesis. Analysis of the targets of the differentially expressed miRNAs revealed novel pathways in erythroid differentiation. Finally, we described the application of Clustered regularly interspaced short palindromic repeats-Cas9 (CRISPR-Cas9) based editing of miRNAs to study their function in human erythropoiesis.
format	article
author	Aneesha Nath Janakiram Rayabaram Smitha Ijee Abhirup Bagchi Anurag Dutta Chaudhury Debanjan Roy Karthik Chambayil Jyoti Singh Yukio Nakamura Shaji R. Velayudhan
author_facet	Aneesha Nath Janakiram Rayabaram Smitha Ijee Abhirup Bagchi Anurag Dutta Chaudhury Debanjan Roy Karthik Chambayil Jyoti Singh Yukio Nakamura Shaji R. Velayudhan
author_sort	Aneesha Nath
title	Comprehensive Analysis of microRNAs in Human Adult Erythropoiesis
title_short	Comprehensive Analysis of microRNAs in Human Adult Erythropoiesis
title_full	Comprehensive Analysis of microRNAs in Human Adult Erythropoiesis
title_fullStr	Comprehensive Analysis of microRNAs in Human Adult Erythropoiesis
title_full_unstemmed	Comprehensive Analysis of microRNAs in Human Adult Erythropoiesis
title_sort	comprehensive analysis of micrornas in human adult erythropoiesis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1833210d1d9f49d296cdd7d654be51af
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Comprehensive Analysis of microRNAs in Human Adult Erythropoiesis

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