Photobiomodulation as an antioxidant substitute in post-thawing trauma of human stem cells from the apical papilla

Abstract Cryopreservation, the most common method of preserving stem cells, requires post-processing because it produces trauma to the cells. Post-thawing trauma typically induces cell death, elevates reactive oxygen species (ROS) concentration, and lowers mitochondrial membrane potential (MMP). Alt...

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Autores principales:	Woori Choi, Ku Youn Baik, Seung Jeong, Sangbae Park, Jae Eun Kim, Hong Bae Kim, Jong Hoon Chung
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1835eb6bf0f84f97b9d49cd02c8f2965
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spelling	oai:doaj.org-article:1835eb6bf0f84f97b9d49cd02c8f29652021-12-02T17:51:21ZPhotobiomodulation as an antioxidant substitute in post-thawing trauma of human stem cells from the apical papilla10.1038/s41598-021-96841-32045-2322https://doaj.org/article/1835eb6bf0f84f97b9d49cd02c8f29652021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96841-3https://doaj.org/toc/2045-2322Abstract Cryopreservation, the most common method of preserving stem cells, requires post-processing because it produces trauma to the cells. Post-thawing trauma typically induces cell death, elevates reactive oxygen species (ROS) concentration, and lowers mitochondrial membrane potential (MMP). Although this trauma has been solved using antioxidants, we attempted to use photobiomodulation (PBM) instead of chemical treatment. We used a 950-nm near-infrared LED to create a PBM device and chose a pulsed-wave mode of 30 Hz and a 30% duty cycle. Near-infrared radiation (NIR) at 950 nm was effective in reducing cell death caused by hydrogen peroxide induced-oxidative stress. Cryodamage also leads to apoptosis of cells, which can be avoided by irradiation at 950 nm NIR. Irradiation as post-processing for cryopreservation had an antioxidant effect that reduced both cellular and mitochondrial ROS. It also increased mitochondrial mass and activated mitochondrial activity, resulting in increased MMP, ATP generation, and increased cytochrome c oxidase activity. In addition, NIR increased alkaline phosphatase (ALP) activity, a biomarker of differentiation. As a result, we identified that 950 nm NIR PBM solves cryodamage in human stem cells from the apical papilla, indicating its potential as an alternative to antioxidants for treatment of post-thawing trauma, and further estimated its mechanism.Woori ChoiKu Youn BaikSeung JeongSangbae ParkJae Eun KimHong Bae KimJong Hoon ChungNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Woori Choi Ku Youn Baik Seung Jeong Sangbae Park Jae Eun Kim Hong Bae Kim Jong Hoon Chung Photobiomodulation as an antioxidant substitute in post-thawing trauma of human stem cells from the apical papilla
description	Abstract Cryopreservation, the most common method of preserving stem cells, requires post-processing because it produces trauma to the cells. Post-thawing trauma typically induces cell death, elevates reactive oxygen species (ROS) concentration, and lowers mitochondrial membrane potential (MMP). Although this trauma has been solved using antioxidants, we attempted to use photobiomodulation (PBM) instead of chemical treatment. We used a 950-nm near-infrared LED to create a PBM device and chose a pulsed-wave mode of 30 Hz and a 30% duty cycle. Near-infrared radiation (NIR) at 950 nm was effective in reducing cell death caused by hydrogen peroxide induced-oxidative stress. Cryodamage also leads to apoptosis of cells, which can be avoided by irradiation at 950 nm NIR. Irradiation as post-processing for cryopreservation had an antioxidant effect that reduced both cellular and mitochondrial ROS. It also increased mitochondrial mass and activated mitochondrial activity, resulting in increased MMP, ATP generation, and increased cytochrome c oxidase activity. In addition, NIR increased alkaline phosphatase (ALP) activity, a biomarker of differentiation. As a result, we identified that 950 nm NIR PBM solves cryodamage in human stem cells from the apical papilla, indicating its potential as an alternative to antioxidants for treatment of post-thawing trauma, and further estimated its mechanism.
format	article
author	Woori Choi Ku Youn Baik Seung Jeong Sangbae Park Jae Eun Kim Hong Bae Kim Jong Hoon Chung
author_facet	Woori Choi Ku Youn Baik Seung Jeong Sangbae Park Jae Eun Kim Hong Bae Kim Jong Hoon Chung
author_sort	Woori Choi
title	Photobiomodulation as an antioxidant substitute in post-thawing trauma of human stem cells from the apical papilla
title_short	Photobiomodulation as an antioxidant substitute in post-thawing trauma of human stem cells from the apical papilla
title_full	Photobiomodulation as an antioxidant substitute in post-thawing trauma of human stem cells from the apical papilla
title_fullStr	Photobiomodulation as an antioxidant substitute in post-thawing trauma of human stem cells from the apical papilla
title_full_unstemmed	Photobiomodulation as an antioxidant substitute in post-thawing trauma of human stem cells from the apical papilla
title_sort	photobiomodulation as an antioxidant substitute in post-thawing trauma of human stem cells from the apical papilla
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1835eb6bf0f84f97b9d49cd02c8f2965
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Photobiomodulation as an antioxidant substitute in post-thawing trauma of human stem cells from the apical papilla

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