3D Bioprinting of Pectin-Cellulose Nanofibers Multicomponent Bioinks

Pectin has found extensive interest in biomedical applications, including wound dressing, drug delivery, and cancer targeting. However, the low viscosity of pectin solutions hinders their applications in 3D bioprinting. Here, we developed multicomponent bioinks prepared by combining pectin with TEMP...

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Autores principales:	Matteo Pitton, Andrea Fiorati, Silvia Buscemi, Lucio Melone, Silvia Farè, Nicola Contessi Negrini
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	pectin cellulose nanofiber hydrogel bioprinting multicomponent bioink 3D printing Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/1836242337694e5485bf8378e6bfd878
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spelling	oai:doaj.org-article:1836242337694e5485bf8378e6bfd8782021-12-03T05:46:20Z3D Bioprinting of Pectin-Cellulose Nanofibers Multicomponent Bioinks2296-418510.3389/fbioe.2021.732689https://doaj.org/article/1836242337694e5485bf8378e6bfd8782021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.732689/fullhttps://doaj.org/toc/2296-4185Pectin has found extensive interest in biomedical applications, including wound dressing, drug delivery, and cancer targeting. However, the low viscosity of pectin solutions hinders their applications in 3D bioprinting. Here, we developed multicomponent bioinks prepared by combining pectin with TEMPO-oxidized cellulose nanofibers (TOCNFs) to optimize the inks’ printability while ensuring stability of the printed hydrogels and simultaneously print viable cell-laden inks. First, we screened several combinations of pectin (1%, 1.5%, 2%, and 2.5% w/v) and TOCNFs (0%, 0.5%, 1%, and 1.5% w/v) by testing their rheological properties and printability. Addition of TOCNFs allowed increasing the inks’ viscosity while maintaining shear thinning rheological response, and it allowed us to identify the optimal pectin concentration (2.5% w/v). We then selected the optimal TOCNFs concentration (1% w/v) by evaluating the viability of cells embedded in the ink and eventually optimized the writing speed to be used to print accurate 3D grid structures. Bioinks were prepared by embedding L929 fibroblast cells in the ink printed by optimized printing parameters. The printed scaffolds were stable in a physiological-like environment and characterized by an elastic modulus of E = 1.8 ± 0.2 kPa. Cells loaded in the ink and printed were viable (cell viability >80%) and their metabolic activity increased in time during the in vitro culture, showing the potential use of the developed bioinks for biofabrication and tissue engineering applications.Matteo PittonMatteo PittonAndrea FioratiAndrea FioratiSilvia BuscemiLucio MeloneLucio MeloneLucio MeloneSilvia FarèSilvia FarèNicola Contessi NegriniNicola Contessi NegriniFrontiers Media S.A.articlepectincellulose nanofiberhydrogelbioprintingmulticomponent bioink3D printingBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pectin cellulose nanofiber hydrogel bioprinting multicomponent bioink 3D printing Biotechnology TP248.13-248.65
spellingShingle	pectin cellulose nanofiber hydrogel bioprinting multicomponent bioink 3D printing Biotechnology TP248.13-248.65 Matteo Pitton Matteo Pitton Andrea Fiorati Andrea Fiorati Silvia Buscemi Lucio Melone Lucio Melone Lucio Melone Silvia Farè Silvia Farè Nicola Contessi Negrini Nicola Contessi Negrini 3D Bioprinting of Pectin-Cellulose Nanofibers Multicomponent Bioinks
description	Pectin has found extensive interest in biomedical applications, including wound dressing, drug delivery, and cancer targeting. However, the low viscosity of pectin solutions hinders their applications in 3D bioprinting. Here, we developed multicomponent bioinks prepared by combining pectin with TEMPO-oxidized cellulose nanofibers (TOCNFs) to optimize the inks’ printability while ensuring stability of the printed hydrogels and simultaneously print viable cell-laden inks. First, we screened several combinations of pectin (1%, 1.5%, 2%, and 2.5% w/v) and TOCNFs (0%, 0.5%, 1%, and 1.5% w/v) by testing their rheological properties and printability. Addition of TOCNFs allowed increasing the inks’ viscosity while maintaining shear thinning rheological response, and it allowed us to identify the optimal pectin concentration (2.5% w/v). We then selected the optimal TOCNFs concentration (1% w/v) by evaluating the viability of cells embedded in the ink and eventually optimized the writing speed to be used to print accurate 3D grid structures. Bioinks were prepared by embedding L929 fibroblast cells in the ink printed by optimized printing parameters. The printed scaffolds were stable in a physiological-like environment and characterized by an elastic modulus of E = 1.8 ± 0.2 kPa. Cells loaded in the ink and printed were viable (cell viability >80%) and their metabolic activity increased in time during the in vitro culture, showing the potential use of the developed bioinks for biofabrication and tissue engineering applications.
format	article
author	Matteo Pitton Matteo Pitton Andrea Fiorati Andrea Fiorati Silvia Buscemi Lucio Melone Lucio Melone Lucio Melone Silvia Farè Silvia Farè Nicola Contessi Negrini Nicola Contessi Negrini
author_facet	Matteo Pitton Matteo Pitton Andrea Fiorati Andrea Fiorati Silvia Buscemi Lucio Melone Lucio Melone Lucio Melone Silvia Farè Silvia Farè Nicola Contessi Negrini Nicola Contessi Negrini
author_sort	Matteo Pitton
title	3D Bioprinting of Pectin-Cellulose Nanofibers Multicomponent Bioinks
title_short	3D Bioprinting of Pectin-Cellulose Nanofibers Multicomponent Bioinks
title_full	3D Bioprinting of Pectin-Cellulose Nanofibers Multicomponent Bioinks
title_fullStr	3D Bioprinting of Pectin-Cellulose Nanofibers Multicomponent Bioinks
title_full_unstemmed	3D Bioprinting of Pectin-Cellulose Nanofibers Multicomponent Bioinks
title_sort	3d bioprinting of pectin-cellulose nanofibers multicomponent bioinks
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1836242337694e5485bf8378e6bfd878
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3D Bioprinting of Pectin-Cellulose Nanofibers Multicomponent Bioinks

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