Silencing FYVE, RhoGEF and PH domain containing 1 (FGD1) suppresses melanoma progression by inhibiting PI3K/AKT signaling pathway

Cutaneous melanoma is the leading cause of death among skin cancers despite the availability of diverse treatments. FGD1 plays an important role in multiple cancers, but how it works in cutaneous melanoma has not been illustrated. Thus, this study was intended to investigate the roles of FGD1 and it...

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Autores principales:	Zehao Niu, Yan Li, Yujian Xu, Weiqian Jiang, Ran Tao, Youbai Chen, Yan Han
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	fgd1 pi3k akt melanoma prognostic factor biomarker Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/1840934e97724acd9d08b9d9c26f125e
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spelling	oai:doaj.org-article:1840934e97724acd9d08b9d9c26f125e2021-11-17T14:21:59ZSilencing FYVE, RhoGEF and PH domain containing 1 (FGD1) suppresses melanoma progression by inhibiting PI3K/AKT signaling pathway2165-59792165-598710.1080/21655979.2021.2005877https://doaj.org/article/1840934e97724acd9d08b9d9c26f125e2021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2005877https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Cutaneous melanoma is the leading cause of death among skin cancers despite the availability of diverse treatments. FGD1 plays an important role in multiple cancers, but how it works in cutaneous melanoma has not been illustrated. Thus, this study was intended to investigate the roles of FGD1 and its underlying mechanisms in cutaneous melanoma. Bioinformatics tools and quantitative real-time polymerase chain reaction (qRT-PCR) were used to analyze the expression of FGD1 in cutaneous melanoma. After knockdown of FGD1 in melanoma cells, the proliferation, migration and invasion of cells were analyzed by cell counting kit-8 (CCK8) assay, colony formation assay and transwell assays. Western blot was used to check the expression of key factors in PI3K/AKT pathway. In addition, nude mice models were used to study the role of FGD1 in melanoma development and metastasis in vivo. The data demonstrated that FGD1 was up-regulated and predicted a poor clinical outcome for cutaneous melanoma patients. Knockdown of FGD1 inhibited melanoma cell proliferation, migration and invasion. The expressions of p-PI3K and p-AKT were significantly decreased while the expressions of PI3K and AKT showed no marked difference in the knockdown group. Meanwhile, knockdown of FGD1 suppressed the development of melanoma in vivo. This study suggested that knockdown of FGD1 could block melanoma formation and proliferation by inhibiting PI3K/AKT signaling pathway. FGD1 might be a promising threptic target for melanoma.Zehao NiuYan LiYujian XuWeiqian JiangRan TaoYoubai ChenYan HanTaylor & Francis Grouparticlefgd1pi3kaktmelanomaprognostic factorbiomarkerBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fgd1 pi3k akt melanoma prognostic factor biomarker Biotechnology TP248.13-248.65
spellingShingle	fgd1 pi3k akt melanoma prognostic factor biomarker Biotechnology TP248.13-248.65 Zehao Niu Yan Li Yujian Xu Weiqian Jiang Ran Tao Youbai Chen Yan Han Silencing FYVE, RhoGEF and PH domain containing 1 (FGD1) suppresses melanoma progression by inhibiting PI3K/AKT signaling pathway
description	Cutaneous melanoma is the leading cause of death among skin cancers despite the availability of diverse treatments. FGD1 plays an important role in multiple cancers, but how it works in cutaneous melanoma has not been illustrated. Thus, this study was intended to investigate the roles of FGD1 and its underlying mechanisms in cutaneous melanoma. Bioinformatics tools and quantitative real-time polymerase chain reaction (qRT-PCR) were used to analyze the expression of FGD1 in cutaneous melanoma. After knockdown of FGD1 in melanoma cells, the proliferation, migration and invasion of cells were analyzed by cell counting kit-8 (CCK8) assay, colony formation assay and transwell assays. Western blot was used to check the expression of key factors in PI3K/AKT pathway. In addition, nude mice models were used to study the role of FGD1 in melanoma development and metastasis in vivo. The data demonstrated that FGD1 was up-regulated and predicted a poor clinical outcome for cutaneous melanoma patients. Knockdown of FGD1 inhibited melanoma cell proliferation, migration and invasion. The expressions of p-PI3K and p-AKT were significantly decreased while the expressions of PI3K and AKT showed no marked difference in the knockdown group. Meanwhile, knockdown of FGD1 suppressed the development of melanoma in vivo. This study suggested that knockdown of FGD1 could block melanoma formation and proliferation by inhibiting PI3K/AKT signaling pathway. FGD1 might be a promising threptic target for melanoma.
format	article
author	Zehao Niu Yan Li Yujian Xu Weiqian Jiang Ran Tao Youbai Chen Yan Han
author_facet	Zehao Niu Yan Li Yujian Xu Weiqian Jiang Ran Tao Youbai Chen Yan Han
author_sort	Zehao Niu
title	Silencing FYVE, RhoGEF and PH domain containing 1 (FGD1) suppresses melanoma progression by inhibiting PI3K/AKT signaling pathway
title_short	Silencing FYVE, RhoGEF and PH domain containing 1 (FGD1) suppresses melanoma progression by inhibiting PI3K/AKT signaling pathway
title_full	Silencing FYVE, RhoGEF and PH domain containing 1 (FGD1) suppresses melanoma progression by inhibiting PI3K/AKT signaling pathway
title_fullStr	Silencing FYVE, RhoGEF and PH domain containing 1 (FGD1) suppresses melanoma progression by inhibiting PI3K/AKT signaling pathway
title_full_unstemmed	Silencing FYVE, RhoGEF and PH domain containing 1 (FGD1) suppresses melanoma progression by inhibiting PI3K/AKT signaling pathway
title_sort	silencing fyve, rhogef and ph domain containing 1 (fgd1) suppresses melanoma progression by inhibiting pi3k/akt signaling pathway
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/1840934e97724acd9d08b9d9c26f125e
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Silencing FYVE, RhoGEF and PH domain containing 1 (FGD1) suppresses melanoma progression by inhibiting PI3K/AKT signaling pathway

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