A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions

The interrogation of noncoding regions of the genome with CRISPR strategies requires dense and, ideally, inexpensive libraries to ensure coverage and customisation. Here the authors present 'Molecular Chipper' that uses fragmentation to generate guide RNAs from input DNA.

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Autores principales:	Jijun Cheng, Christine A. Roden, Wen Pan, Shu Zhu, Anna Baccei, Xinghua Pan, Tingting Jiang, Yuval Kluger, Sherman M. Weissman, Shangqin Guo, Richard A. Flavell, Ye Ding, Jun Lu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/18430b661da84ddb83ebbefbef3dc891
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spelling	oai:doaj.org-article:18430b661da84ddb83ebbefbef3dc8912021-12-02T15:34:58ZA Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions10.1038/ncomms111782041-1723https://doaj.org/article/18430b661da84ddb83ebbefbef3dc8912016-03-01T00:00:00Zhttps://doi.org/10.1038/ncomms11178https://doaj.org/toc/2041-1723The interrogation of noncoding regions of the genome with CRISPR strategies requires dense and, ideally, inexpensive libraries to ensure coverage and customisation. Here the authors present 'Molecular Chipper' that uses fragmentation to generate guide RNAs from input DNA.Jijun ChengChristine A. RodenWen PanShu ZhuAnna BacceiXinghua PanTingting JiangYuval KlugerSherman M. WeissmanShangqin GuoRichard A. FlavellYe DingJun LuNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-10 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jijun Cheng Christine A. Roden Wen Pan Shu Zhu Anna Baccei Xinghua Pan Tingting Jiang Yuval Kluger Sherman M. Weissman Shangqin Guo Richard A. Flavell Ye Ding Jun Lu A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions
description	The interrogation of noncoding regions of the genome with CRISPR strategies requires dense and, ideally, inexpensive libraries to ensure coverage and customisation. Here the authors present 'Molecular Chipper' that uses fragmentation to generate guide RNAs from input DNA.
format	article
author	Jijun Cheng Christine A. Roden Wen Pan Shu Zhu Anna Baccei Xinghua Pan Tingting Jiang Yuval Kluger Sherman M. Weissman Shangqin Guo Richard A. Flavell Ye Ding Jun Lu
author_facet	Jijun Cheng Christine A. Roden Wen Pan Shu Zhu Anna Baccei Xinghua Pan Tingting Jiang Yuval Kluger Sherman M. Weissman Shangqin Guo Richard A. Flavell Ye Ding Jun Lu
author_sort	Jijun Cheng
title	A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions
title_short	A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions
title_full	A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions
title_fullStr	A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions
title_full_unstemmed	A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions
title_sort	molecular chipper technology for crispr sgrna library generation and functional mapping of noncoding regions
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/18430b661da84ddb83ebbefbef3dc891
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A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions

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