Mechanosensitive Piezo1 in Periodontal Ligament Cells Promotes Alveolar Bone Remodeling During Orthodontic Tooth Movement

Orthodontic tooth movement (OTM) is a process depending on the remodeling of periodontal tissues surrounding the roots. Orthodontic forces trigger the conversion of mechanical stimuli into intercellular chemical signals within periodontal ligament (PDL) cells, activating alveolar bone remodeling, an...

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Autores principales:	Yukun Jiang, Yuzhe Guan, Yuanchen Lan, Shuo Chen, Tiancheng Li, Shujuan Zou, Zhiai Hu, Qingsong Ye
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Piezo1 bone remodeling orthodontic tooth movement alveolar bone mechanotransduction Physiology QP1-981
Acceso en línea:	https://doaj.org/article/1843bf760f1f4e4299db6afe1bfe34af
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spelling	oai:doaj.org-article:1843bf760f1f4e4299db6afe1bfe34af2021-11-22T06:55:37ZMechanosensitive Piezo1 in Periodontal Ligament Cells Promotes Alveolar Bone Remodeling During Orthodontic Tooth Movement1664-042X10.3389/fphys.2021.767136https://doaj.org/article/1843bf760f1f4e4299db6afe1bfe34af2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphys.2021.767136/fullhttps://doaj.org/toc/1664-042XOrthodontic tooth movement (OTM) is a process depending on the remodeling of periodontal tissues surrounding the roots. Orthodontic forces trigger the conversion of mechanical stimuli into intercellular chemical signals within periodontal ligament (PDL) cells, activating alveolar bone remodeling, and thereby, initiating OTM. Recently, the mechanosensitive ion channel Piezo1 has been found to play pivotal roles in the different types of human cells by transforming external physical stimuli into intercellular chemical signals. However, the function of Piezo1 during the mechanotransduction process of PDL cells has rarely been reported. Herein, we established a rat OTM model to study the potential role of Piezo1 during the mechanotransduction process of PDL cells and investigate its effects on the tension side of alveolar bone remodeling. A total of 60 male Sprague-Dawley rats were randomly assigned into three groups: the OTM + inhibitor (INH) group, the OTM group, and the control (CON) group. Nickel-titanium orthodontic springs were applied to trigger tooth movement. Mice were sacrificed on days 0, 3, 7, and 14 after orthodontic movement for the radiographic, histological, immunohistochemical, and molecular biological analyses. Our results revealed that the Piezo1 channel was activated by orthodontic force and mainly expressed in the PDL cells during the whole tooth movement period. The activation of the Piezo1 channel was essential for maintaining the rate of orthodontic tooth movement and facilitation of new alveolar bone formation on the tension side. Reduced osteogenesis-associated transcription factors such as Runt-related transcription factor 2 (RUNX2), Osterix (OSX), and receptor activator of nuclear factor-kappa B ligand (RANKL)/osteoprotegerin (OPG) ratio were examined when the function of Piezo1 was inhibited. In summary, Piezo1 plays a critical role in mediating both the osteogenesis and osteoclastic activities on the tension side during OTM.Yukun JiangYuzhe GuanYuanchen LanShuo ChenTiancheng LiShujuan ZouZhiai HuQingsong YeQingsong YeFrontiers Media S.A.articlePiezo1bone remodelingorthodontic tooth movementalveolar bonemechanotransductionPhysiologyQP1-981ENFrontiers in Physiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Piezo1 bone remodeling orthodontic tooth movement alveolar bone mechanotransduction Physiology QP1-981
spellingShingle	Piezo1 bone remodeling orthodontic tooth movement alveolar bone mechanotransduction Physiology QP1-981 Yukun Jiang Yuzhe Guan Yuanchen Lan Shuo Chen Tiancheng Li Shujuan Zou Zhiai Hu Qingsong Ye Qingsong Ye Mechanosensitive Piezo1 in Periodontal Ligament Cells Promotes Alveolar Bone Remodeling During Orthodontic Tooth Movement
description	Orthodontic tooth movement (OTM) is a process depending on the remodeling of periodontal tissues surrounding the roots. Orthodontic forces trigger the conversion of mechanical stimuli into intercellular chemical signals within periodontal ligament (PDL) cells, activating alveolar bone remodeling, and thereby, initiating OTM. Recently, the mechanosensitive ion channel Piezo1 has been found to play pivotal roles in the different types of human cells by transforming external physical stimuli into intercellular chemical signals. However, the function of Piezo1 during the mechanotransduction process of PDL cells has rarely been reported. Herein, we established a rat OTM model to study the potential role of Piezo1 during the mechanotransduction process of PDL cells and investigate its effects on the tension side of alveolar bone remodeling. A total of 60 male Sprague-Dawley rats were randomly assigned into three groups: the OTM + inhibitor (INH) group, the OTM group, and the control (CON) group. Nickel-titanium orthodontic springs were applied to trigger tooth movement. Mice were sacrificed on days 0, 3, 7, and 14 after orthodontic movement for the radiographic, histological, immunohistochemical, and molecular biological analyses. Our results revealed that the Piezo1 channel was activated by orthodontic force and mainly expressed in the PDL cells during the whole tooth movement period. The activation of the Piezo1 channel was essential for maintaining the rate of orthodontic tooth movement and facilitation of new alveolar bone formation on the tension side. Reduced osteogenesis-associated transcription factors such as Runt-related transcription factor 2 (RUNX2), Osterix (OSX), and receptor activator of nuclear factor-kappa B ligand (RANKL)/osteoprotegerin (OPG) ratio were examined when the function of Piezo1 was inhibited. In summary, Piezo1 plays a critical role in mediating both the osteogenesis and osteoclastic activities on the tension side during OTM.
format	article
author	Yukun Jiang Yuzhe Guan Yuanchen Lan Shuo Chen Tiancheng Li Shujuan Zou Zhiai Hu Qingsong Ye Qingsong Ye
author_facet	Yukun Jiang Yuzhe Guan Yuanchen Lan Shuo Chen Tiancheng Li Shujuan Zou Zhiai Hu Qingsong Ye Qingsong Ye
author_sort	Yukun Jiang
title	Mechanosensitive Piezo1 in Periodontal Ligament Cells Promotes Alveolar Bone Remodeling During Orthodontic Tooth Movement
title_short	Mechanosensitive Piezo1 in Periodontal Ligament Cells Promotes Alveolar Bone Remodeling During Orthodontic Tooth Movement
title_full	Mechanosensitive Piezo1 in Periodontal Ligament Cells Promotes Alveolar Bone Remodeling During Orthodontic Tooth Movement
title_fullStr	Mechanosensitive Piezo1 in Periodontal Ligament Cells Promotes Alveolar Bone Remodeling During Orthodontic Tooth Movement
title_full_unstemmed	Mechanosensitive Piezo1 in Periodontal Ligament Cells Promotes Alveolar Bone Remodeling During Orthodontic Tooth Movement
title_sort	mechanosensitive piezo1 in periodontal ligament cells promotes alveolar bone remodeling during orthodontic tooth movement
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1843bf760f1f4e4299db6afe1bfe34af
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Mechanosensitive Piezo1 in Periodontal Ligament Cells Promotes Alveolar Bone Remodeling During Orthodontic Tooth Movement

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