Major Vault Protein Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection in CRL2843<sup>CD163</sup> Cell Lines and Primary Porcine Alveolar Macrophages

Porcine reproductive and respiratory syndrome (PRRS), a significant viral infectious disease that commonly occurs among farmed pigs, leads to considerable economic losses to the swine industry worldwide. Major vault protein (MVP) is a host factor that induces type Ⅰ interferon (IFN) production. In t...

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Autores principales:	Xiaoping Wu, Junyang Fang, Qiuping Huang, Xu Chen, Zhongyi Guo, Lingyujia Tian, Enmin Zhou, Jianxin Chen, Yang Mu, Taofeng Du
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	PRRSV MVP host factor type Ⅰ interferon antiviral Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/18481193356e4a7cb8269bf14c21611d
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spelling	oai:doaj.org-article:18481193356e4a7cb8269bf14c21611d2021-11-25T19:13:56ZMajor Vault Protein Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection in CRL2843<sup>CD163</sup> Cell Lines and Primary Porcine Alveolar Macrophages10.3390/v131122671999-4915https://doaj.org/article/18481193356e4a7cb8269bf14c21611d2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2267https://doaj.org/toc/1999-4915Porcine reproductive and respiratory syndrome (PRRS), a significant viral infectious disease that commonly occurs among farmed pigs, leads to considerable economic losses to the swine industry worldwide. Major vault protein (MVP) is a host factor that induces type Ⅰ interferon (IFN) production. In this study, we evaluated the effect of MVP on PRRSV infection in CRL2843<sup>CD163</sup> cell lines and porcine alveolar macrophages (PAMs). Our results showed that MVP expression was downregulated by PRRSV infection. Adenoviral overexpression of MVP inhibited PRRSV replication, whereas the siRNA knockdown of MVP promoted PRRSV replication. In addition, MVP knockdown has an adverse effect on the inhibitive role of MVP overexpression on PRRSV replication. Moreover, MVP could induce the expression of type Ⅰ IFNs and IFN-stimulated gene 15 (ISG15) in PRRSV-infected PAMs. Based on these results, MVP may be a potential molecular target of drugs for the effective prevention and treatment of PRRSV infection.Xiaoping WuJunyang FangQiuping HuangXu ChenZhongyi GuoLingyujia TianEnmin ZhouJianxin ChenYang MuTaofeng DuMDPI AGarticlePRRSVMVPhost factortype Ⅰ interferonantiviralMicrobiologyQR1-502ENViruses, Vol 13, Iss 2267, p 2267 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	PRRSV MVP host factor type Ⅰ interferon antiviral Microbiology QR1-502
spellingShingle	PRRSV MVP host factor type Ⅰ interferon antiviral Microbiology QR1-502 Xiaoping Wu Junyang Fang Qiuping Huang Xu Chen Zhongyi Guo Lingyujia Tian Enmin Zhou Jianxin Chen Yang Mu Taofeng Du Major Vault Protein Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection in CRL2843<sup>CD163</sup> Cell Lines and Primary Porcine Alveolar Macrophages
description	Porcine reproductive and respiratory syndrome (PRRS), a significant viral infectious disease that commonly occurs among farmed pigs, leads to considerable economic losses to the swine industry worldwide. Major vault protein (MVP) is a host factor that induces type Ⅰ interferon (IFN) production. In this study, we evaluated the effect of MVP on PRRSV infection in CRL2843<sup>CD163</sup> cell lines and porcine alveolar macrophages (PAMs). Our results showed that MVP expression was downregulated by PRRSV infection. Adenoviral overexpression of MVP inhibited PRRSV replication, whereas the siRNA knockdown of MVP promoted PRRSV replication. In addition, MVP knockdown has an adverse effect on the inhibitive role of MVP overexpression on PRRSV replication. Moreover, MVP could induce the expression of type Ⅰ IFNs and IFN-stimulated gene 15 (ISG15) in PRRSV-infected PAMs. Based on these results, MVP may be a potential molecular target of drugs for the effective prevention and treatment of PRRSV infection.
format	article
author	Xiaoping Wu Junyang Fang Qiuping Huang Xu Chen Zhongyi Guo Lingyujia Tian Enmin Zhou Jianxin Chen Yang Mu Taofeng Du
author_facet	Xiaoping Wu Junyang Fang Qiuping Huang Xu Chen Zhongyi Guo Lingyujia Tian Enmin Zhou Jianxin Chen Yang Mu Taofeng Du
author_sort	Xiaoping Wu
title	Major Vault Protein Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection in CRL2843<sup>CD163</sup> Cell Lines and Primary Porcine Alveolar Macrophages
title_short	Major Vault Protein Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection in CRL2843<sup>CD163</sup> Cell Lines and Primary Porcine Alveolar Macrophages
title_full	Major Vault Protein Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection in CRL2843<sup>CD163</sup> Cell Lines and Primary Porcine Alveolar Macrophages
title_fullStr	Major Vault Protein Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection in CRL2843<sup>CD163</sup> Cell Lines and Primary Porcine Alveolar Macrophages
title_full_unstemmed	Major Vault Protein Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection in CRL2843<sup>CD163</sup> Cell Lines and Primary Porcine Alveolar Macrophages
title_sort	major vault protein inhibits porcine reproductive and respiratory syndrome virus infection in crl2843<sup>cd163</sup> cell lines and primary porcine alveolar macrophages
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/18481193356e4a7cb8269bf14c21611d
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Major Vault Protein Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection in CRL2843<sup>CD163</sup> Cell Lines and Primary Porcine Alveolar Macrophages

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