A comprehensive survey of miRNA repertoire and 3' addition events in the placentas of patients with pre-eclampsia from high-throughput sequencing.

<h4>Background</h4>To gain insight into potential roles of isomiR spectrum and isomiRs with 3' additions in pre-eclampsia, we performed a comprehensive survey of miRNA repertoire and 3' addition events from placental samples with different degrees of pre-eclampsia by applying S...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Li Guo, Qi Yang, Jiafeng Lu, Hailing Li, Qinyu Ge, Wanjun Gu, Yunfei Bai, Zuhong Lu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/184c3cec99fa476ba7045e0dc28ea2ad
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:184c3cec99fa476ba7045e0dc28ea2ad
record_format	dspace
spelling	oai:doaj.org-article:184c3cec99fa476ba7045e0dc28ea2ad2021-11-18T06:51:34ZA comprehensive survey of miRNA repertoire and 3' addition events in the placentas of patients with pre-eclampsia from high-throughput sequencing.1932-620310.1371/journal.pone.0021072https://doaj.org/article/184c3cec99fa476ba7045e0dc28ea2ad2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21731650/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>To gain insight into potential roles of isomiR spectrum and isomiRs with 3' additions in pre-eclampsia, we performed a comprehensive survey of miRNA repertoire and 3' addition events from placental samples with different degrees of pre-eclampsia by applying SOLiD sequencing platform.<h4>Principal findings</h4>Over 30% isomiRs were detected with 3' non-template additional nucleotides, especially for additional nucleotide of adenosine. However, these modified isomiRs showed a lower percentage of total miRNA expression (<15%). Generally, 1-3 abundant isomiRs from a given miRNA locus were identified, but none of them was detected with 3' additions. Different miRNAs indicated various isomiR spectrums and expression patterns. The most abundant isomiR spectrum, isomiR profile and expression pattern always were stability, but herein we found several exceptions across samples, especially between normal and diseased samples. At isomiR level, we detected a distinct subset of differentially expressed modified isomiRs between normal and diseased samples or between mild and severe samples. Gene Ontology analysis of their experimentally validated target genes revealed enrichment for specific biological process categories.<h4>Conclusions</h4>The phenomenon of multiple isomiRs, especially for isomiRs with 3' additions, is not a random event during pre-miRNA processing. Varieties of isomiRs and expression patterns reveal potential functional implication and should be taken into account. The study enriches association of miRNAs and human disease, including potential roles of various miRNA variants and 3' addition events.Li GuoQi YangJiafeng LuHailing LiQinyu GeWanjun GuYunfei BaiZuhong LuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 6, p e21072 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Li Guo Qi Yang Jiafeng Lu Hailing Li Qinyu Ge Wanjun Gu Yunfei Bai Zuhong Lu A comprehensive survey of miRNA repertoire and 3' addition events in the placentas of patients with pre-eclampsia from high-throughput sequencing.
description	<h4>Background</h4>To gain insight into potential roles of isomiR spectrum and isomiRs with 3' additions in pre-eclampsia, we performed a comprehensive survey of miRNA repertoire and 3' addition events from placental samples with different degrees of pre-eclampsia by applying SOLiD sequencing platform.<h4>Principal findings</h4>Over 30% isomiRs were detected with 3' non-template additional nucleotides, especially for additional nucleotide of adenosine. However, these modified isomiRs showed a lower percentage of total miRNA expression (<15%). Generally, 1-3 abundant isomiRs from a given miRNA locus were identified, but none of them was detected with 3' additions. Different miRNAs indicated various isomiR spectrums and expression patterns. The most abundant isomiR spectrum, isomiR profile and expression pattern always were stability, but herein we found several exceptions across samples, especially between normal and diseased samples. At isomiR level, we detected a distinct subset of differentially expressed modified isomiRs between normal and diseased samples or between mild and severe samples. Gene Ontology analysis of their experimentally validated target genes revealed enrichment for specific biological process categories.<h4>Conclusions</h4>The phenomenon of multiple isomiRs, especially for isomiRs with 3' additions, is not a random event during pre-miRNA processing. Varieties of isomiRs and expression patterns reveal potential functional implication and should be taken into account. The study enriches association of miRNAs and human disease, including potential roles of various miRNA variants and 3' addition events.
format	article
author	Li Guo Qi Yang Jiafeng Lu Hailing Li Qinyu Ge Wanjun Gu Yunfei Bai Zuhong Lu
author_facet	Li Guo Qi Yang Jiafeng Lu Hailing Li Qinyu Ge Wanjun Gu Yunfei Bai Zuhong Lu
author_sort	Li Guo
title	A comprehensive survey of miRNA repertoire and 3' addition events in the placentas of patients with pre-eclampsia from high-throughput sequencing.
title_short	A comprehensive survey of miRNA repertoire and 3' addition events in the placentas of patients with pre-eclampsia from high-throughput sequencing.
title_full	A comprehensive survey of miRNA repertoire and 3' addition events in the placentas of patients with pre-eclampsia from high-throughput sequencing.
title_fullStr	A comprehensive survey of miRNA repertoire and 3' addition events in the placentas of patients with pre-eclampsia from high-throughput sequencing.
title_full_unstemmed	A comprehensive survey of miRNA repertoire and 3' addition events in the placentas of patients with pre-eclampsia from high-throughput sequencing.
title_sort	comprehensive survey of mirna repertoire and 3' addition events in the placentas of patients with pre-eclampsia from high-throughput sequencing.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/184c3cec99fa476ba7045e0dc28ea2ad
work_keys_str_mv	AT liguo acomprehensivesurveyofmirnarepertoireand3additioneventsintheplacentasofpatientswithpreeclampsiafromhighthroughputsequencing AT qiyang acomprehensivesurveyofmirnarepertoireand3additioneventsintheplacentasofpatientswithpreeclampsiafromhighthroughputsequencing AT jiafenglu acomprehensivesurveyofmirnarepertoireand3additioneventsintheplacentasofpatientswithpreeclampsiafromhighthroughputsequencing AT hailingli acomprehensivesurveyofmirnarepertoireand3additioneventsintheplacentasofpatientswithpreeclampsiafromhighthroughputsequencing AT qinyuge acomprehensivesurveyofmirnarepertoireand3additioneventsintheplacentasofpatientswithpreeclampsiafromhighthroughputsequencing AT wanjungu acomprehensivesurveyofmirnarepertoireand3additioneventsintheplacentasofpatientswithpreeclampsiafromhighthroughputsequencing AT yunfeibai acomprehensivesurveyofmirnarepertoireand3additioneventsintheplacentasofpatientswithpreeclampsiafromhighthroughputsequencing AT zuhonglu acomprehensivesurveyofmirnarepertoireand3additioneventsintheplacentasofpatientswithpreeclampsiafromhighthroughputsequencing AT liguo comprehensivesurveyofmirnarepertoireand3additioneventsintheplacentasofpatientswithpreeclampsiafromhighthroughputsequencing AT qiyang comprehensivesurveyofmirnarepertoireand3additioneventsintheplacentasofpatientswithpreeclampsiafromhighthroughputsequencing AT jiafenglu comprehensivesurveyofmirnarepertoireand3additioneventsintheplacentasofpatientswithpreeclampsiafromhighthroughputsequencing AT hailingli comprehensivesurveyofmirnarepertoireand3additioneventsintheplacentasofpatientswithpreeclampsiafromhighthroughputsequencing AT qinyuge comprehensivesurveyofmirnarepertoireand3additioneventsintheplacentasofpatientswithpreeclampsiafromhighthroughputsequencing AT wanjungu comprehensivesurveyofmirnarepertoireand3additioneventsintheplacentasofpatientswithpreeclampsiafromhighthroughputsequencing AT yunfeibai comprehensivesurveyofmirnarepertoireand3additioneventsintheplacentasofpatientswithpreeclampsiafromhighthroughputsequencing AT zuhonglu comprehensivesurveyofmirnarepertoireand3additioneventsintheplacentasofpatientswithpreeclampsiafromhighthroughputsequencing
_version_	1718424274586304512

A comprehensive survey of miRNA repertoire and 3' addition events in the placentas of patients with pre-eclampsia from high-throughput sequencing.

Ejemplares similares