Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model
Natalia Kurantowicz,1 Ewa Sawosz,1 Gabriela Halik,1 Barbara Strojny,1 Anna Hotowy,1 Marta Grodzik,1 Radosław Piast,2 Wanvimol Pasanphan,3 André Chwalibog4 1Department of Animal Nutrition and Biotechnology, Warsaw University of Life Sciences, 2Faculty of Chemistry, Warsaw University, Wars...
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Dove Medical Press
2017
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oai:doaj.org-article:184fcbcb9fa14e4dad6f9e45d274ec742021-12-02T02:38:20ZToxicity studies of six types of carbon nanoparticles in a chicken-embryo model1178-2013https://doaj.org/article/184fcbcb9fa14e4dad6f9e45d274ec742017-04-01T00:00:00Zhttps://www.dovepress.com/toxicity-studies-of-six-types-of-carbon-nanoparticles-in-a-chicken-emb-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Natalia Kurantowicz,1 Ewa Sawosz,1 Gabriela Halik,1 Barbara Strojny,1 Anna Hotowy,1 Marta Grodzik,1 Radosław Piast,2 Wanvimol Pasanphan,3 André Chwalibog4 1Department of Animal Nutrition and Biotechnology, Warsaw University of Life Sciences, 2Faculty of Chemistry, Warsaw University, Warsaw, Poland; 3Department of Materials Science, Faculty of Science, Kasetsart University, Bangkok, Thailand; 4Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Copenhagen, Denmark Abstract: In the present study, the toxicity of six different types of carbon nanoparticles (CNPs) was investigated using a chicken-embryo model. Fertilized chicken eggs were divided into the following treatment groups: placebo, diamond NPs, graphite NPs, pristine graphene, small graphene oxide, large graphene oxide, and reduced graphene oxide. Experimental solutions at a concentration of 500 µg/mL were administrated into the egg albumin. Gross pathology and the rate of survival were examined after 5, 10, 15, and 20 days of incubation. After 20 days of incubation, blood samples were collected and the weight of the body and organs measured. The relative ratio of embryo survival decreased after treatment all treatments except diamond NPs. There was no correlation between the rate of survival and the ζ-potential or the surface charge of the CNPs in solution. Body and organ weight, red blood-cell morphology, blood serum biochemical parameters, and oxidative damage in the liver did not differ among the groups. These results indicate that CNPs can remain in blood circulation without any major side effects, suggesting their potential applicability as vehicles for drug delivery or active compounds per se. However, there is a need for further investigation of their properties, which vary depending on production methods and surface functionalization. Keywords: nanoparticles, diamond, graphite, graphene, toxicity, red blood cells, oxidative stress, surface chargeKurantowicz NSawosz EHalik GStrojny BHotowy AGrodzik MPiast RPasanphan WChwalibog ADove Medical Pressarticlenanoparticlesdiamondgraphitegraphenetoxicityred blood cellsoxidative stresssurface chargeMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 2887-2898 (2017) |
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nanoparticles diamond graphite graphene toxicity red blood cells oxidative stress surface charge Medicine (General) R5-920 |
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nanoparticles diamond graphite graphene toxicity red blood cells oxidative stress surface charge Medicine (General) R5-920 Kurantowicz N Sawosz E Halik G Strojny B Hotowy A Grodzik M Piast R Pasanphan W Chwalibog A Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model |
| description |
Natalia Kurantowicz,1 Ewa Sawosz,1 Gabriela Halik,1 Barbara Strojny,1 Anna Hotowy,1 Marta Grodzik,1 Radosław Piast,2 Wanvimol Pasanphan,3 André Chwalibog4 1Department of Animal Nutrition and Biotechnology, Warsaw University of Life Sciences, 2Faculty of Chemistry, Warsaw University, Warsaw, Poland; 3Department of Materials Science, Faculty of Science, Kasetsart University, Bangkok, Thailand; 4Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Copenhagen, Denmark Abstract: In the present study, the toxicity of six different types of carbon nanoparticles (CNPs) was investigated using a chicken-embryo model. Fertilized chicken eggs were divided into the following treatment groups: placebo, diamond NPs, graphite NPs, pristine graphene, small graphene oxide, large graphene oxide, and reduced graphene oxide. Experimental solutions at a concentration of 500 µg/mL were administrated into the egg albumin. Gross pathology and the rate of survival were examined after 5, 10, 15, and 20 days of incubation. After 20 days of incubation, blood samples were collected and the weight of the body and organs measured. The relative ratio of embryo survival decreased after treatment all treatments except diamond NPs. There was no correlation between the rate of survival and the ζ-potential or the surface charge of the CNPs in solution. Body and organ weight, red blood-cell morphology, blood serum biochemical parameters, and oxidative damage in the liver did not differ among the groups. These results indicate that CNPs can remain in blood circulation without any major side effects, suggesting their potential applicability as vehicles for drug delivery or active compounds per se. However, there is a need for further investigation of their properties, which vary depending on production methods and surface functionalization. Keywords: nanoparticles, diamond, graphite, graphene, toxicity, red blood cells, oxidative stress, surface charge |
| format |
article |
| author |
Kurantowicz N Sawosz E Halik G Strojny B Hotowy A Grodzik M Piast R Pasanphan W Chwalibog A |
| author_facet |
Kurantowicz N Sawosz E Halik G Strojny B Hotowy A Grodzik M Piast R Pasanphan W Chwalibog A |
| author_sort |
Kurantowicz N |
| title |
Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model |
| title_short |
Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model |
| title_full |
Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model |
| title_fullStr |
Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model |
| title_full_unstemmed |
Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model |
| title_sort |
toxicity studies of six types of carbon nanoparticles in a chicken-embryo model |
| publisher |
Dove Medical Press |
| publishDate |
2017 |
| url |
https://doaj.org/article/184fcbcb9fa14e4dad6f9e45d274ec74 |
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