Multiple begomoviruses found associated with cotton leaf curl disease in Pakistan in early 1990 are back in cultivated cotton

Abstract The first epidemic of cotton leaf curl disease (CLCuD) in early 1990’s in the Indian subcontinent was associated with several distinct begomoviruses along with a disease-specific betasatellite. Resistant cotton varieties were introduced in late 1990’s but soon resistance was broken and was...

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Autores principales:	Muhammad Zubair, Syed Shan-e-Ali Zaidi, Sara Shakir, Muhammad Farooq, Imran Amin, Jodi A. Scheffler, Brian E. Scheffler, Shahid Mansoor
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/185bec225fdd41d48d5493a6c8a8c3aa
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spelling	oai:doaj.org-article:185bec225fdd41d48d5493a6c8a8c3aa2021-12-02T12:32:08ZMultiple begomoviruses found associated with cotton leaf curl disease in Pakistan in early 1990 are back in cultivated cotton10.1038/s41598-017-00727-22045-2322https://doaj.org/article/185bec225fdd41d48d5493a6c8a8c3aa2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00727-2https://doaj.org/toc/2045-2322Abstract The first epidemic of cotton leaf curl disease (CLCuD) in early 1990’s in the Indian subcontinent was associated with several distinct begomoviruses along with a disease-specific betasatellite. Resistant cotton varieties were introduced in late 1990’s but soon resistance was broken and was associated with a single recombinant begomovirus named Burewala strain of Cotton leaf curl Kokhran virus that lacks a full complement of a gene encoding a transcription activator protein (TrAP). In order to understand the ongoing changes in CLCuD complex in Pakistan, CLCuD affected plants from cotton fields at Vehari were collected. Illumina sequencing was used to assess the diversity of CLCuD complex. At least three distinct begomoviruses characterized from the first epidemic; Cotton leaf curl Multan virus, Cotton leaf curl Kokhran virus and Cotton leaf curl Alabad virus, several distinct species of alphasatellites and cotton leaf curl Multan betasatellite were found associated with CLCuD. These viruses were also cloned and sequenced through Sanger sequencing to confirm the identity of the begomoviruses and that all clones possessed a full complement of the TrAP gene. A new strain of betasatellite was identified here and named CLCuMuBVeh. The implications of these findings in efforts to control CLCuD are discussed.Muhammad ZubairSyed Shan-e-Ali ZaidiSara ShakirMuhammad FarooqImran AminJodi A. SchefflerBrian E. SchefflerShahid MansoorNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Muhammad Zubair Syed Shan-e-Ali Zaidi Sara Shakir Muhammad Farooq Imran Amin Jodi A. Scheffler Brian E. Scheffler Shahid Mansoor Multiple begomoviruses found associated with cotton leaf curl disease in Pakistan in early 1990 are back in cultivated cotton
description	Abstract The first epidemic of cotton leaf curl disease (CLCuD) in early 1990’s in the Indian subcontinent was associated with several distinct begomoviruses along with a disease-specific betasatellite. Resistant cotton varieties were introduced in late 1990’s but soon resistance was broken and was associated with a single recombinant begomovirus named Burewala strain of Cotton leaf curl Kokhran virus that lacks a full complement of a gene encoding a transcription activator protein (TrAP). In order to understand the ongoing changes in CLCuD complex in Pakistan, CLCuD affected plants from cotton fields at Vehari were collected. Illumina sequencing was used to assess the diversity of CLCuD complex. At least three distinct begomoviruses characterized from the first epidemic; Cotton leaf curl Multan virus, Cotton leaf curl Kokhran virus and Cotton leaf curl Alabad virus, several distinct species of alphasatellites and cotton leaf curl Multan betasatellite were found associated with CLCuD. These viruses were also cloned and sequenced through Sanger sequencing to confirm the identity of the begomoviruses and that all clones possessed a full complement of the TrAP gene. A new strain of betasatellite was identified here and named CLCuMuBVeh. The implications of these findings in efforts to control CLCuD are discussed.
format	article
author	Muhammad Zubair Syed Shan-e-Ali Zaidi Sara Shakir Muhammad Farooq Imran Amin Jodi A. Scheffler Brian E. Scheffler Shahid Mansoor
author_facet	Muhammad Zubair Syed Shan-e-Ali Zaidi Sara Shakir Muhammad Farooq Imran Amin Jodi A. Scheffler Brian E. Scheffler Shahid Mansoor
author_sort	Muhammad Zubair
title	Multiple begomoviruses found associated with cotton leaf curl disease in Pakistan in early 1990 are back in cultivated cotton
title_short	Multiple begomoviruses found associated with cotton leaf curl disease in Pakistan in early 1990 are back in cultivated cotton
title_full	Multiple begomoviruses found associated with cotton leaf curl disease in Pakistan in early 1990 are back in cultivated cotton
title_fullStr	Multiple begomoviruses found associated with cotton leaf curl disease in Pakistan in early 1990 are back in cultivated cotton
title_full_unstemmed	Multiple begomoviruses found associated with cotton leaf curl disease in Pakistan in early 1990 are back in cultivated cotton
title_sort	multiple begomoviruses found associated with cotton leaf curl disease in pakistan in early 1990 are back in cultivated cotton
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/185bec225fdd41d48d5493a6c8a8c3aa
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Multiple begomoviruses found associated with cotton leaf curl disease in Pakistan in early 1990 are back in cultivated cotton

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