The LORF5 Gene Is Non-essential for Replication but Important for Duck Plague Virus Cell-to-Cell Spread Efficiently in Host Cells
Duck plague virus (DPV) can cause high morbidity and mortality in many waterfowl species within the order Anseriformes. The DPV genome contains 78 open reading frames (ORFs), among which the LORF2, LORF3, LORF4, LORF5, and SORF3 genes are unique genes of avian herpesvirus. In this study, to investig...
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oai:doaj.org-article:1861102915e94b71bee30d074dd1165e2021-12-02T06:22:28ZThe LORF5 Gene Is Non-essential for Replication but Important for Duck Plague Virus Cell-to-Cell Spread Efficiently in Host Cells1664-302X10.3389/fmicb.2021.744408https://doaj.org/article/1861102915e94b71bee30d074dd1165e2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.744408/fullhttps://doaj.org/toc/1664-302XDuck plague virus (DPV) can cause high morbidity and mortality in many waterfowl species within the order Anseriformes. The DPV genome contains 78 open reading frames (ORFs), among which the LORF2, LORF3, LORF4, LORF5, and SORF3 genes are unique genes of avian herpesvirus. In this study, to investigate the role of this unique LORF5 gene in DPV proliferation, we generated a recombinant virus that lacks the LORF5 gene by a two-step red recombination system, which cloned the DPV Chinese virulent strain (DPV CHv) genome into a bacterial artificial chromosome (DPV CHv-BAC); the proliferation law of LORF5-deleted mutant virus on DEF cells and the effect of LORF5 gene on the life cycle stages of DPV compared with the parent strain were tested. Our data revealed that the LORF5 gene contributes to the cell-to-cell transmission of DPV but is not relevant to virus invasion, replication, assembly, and release formation. Taken together, this study sheds light on the role of the avian herpesvirus-specific gene LORF5 in the DPV proliferation life cycle. These findings lay the foundation for in-depth functional studies of the LORF5 gene in DPV or other avian herpesviruses.Bingjie ShenBingjie ShenBingjie ShenYunjiao LiYunjiao LiYunjiao LiAnchun ChengAnchun ChengAnchun ChengMingshu WangMingshu WangMingshu WangYing WuYing WuYing WuQiao YangQiao YangQiao YangRenyong JiaRenyong JiaRenyong JiaBin TianBin TianBin TianXumin OuXumin OuXumin OuSai MaoSai MaoSai MaoDi SunDi SunDi SunShaqiu ZhangShaqiu ZhangShaqiu ZhangDekang ZhuDekang ZhuShun ChenShun ChenShun ChenMafeng LiuMafeng LiuMafeng LiuXin-Xin ZhaoXin-Xin ZhaoXin-Xin ZhaoJuan HuangJuan HuangJuan HuangQun GaoQun GaoQun GaoYunya LiuYunya LiuYunya LiuYanling YuYanling YuYanling YuLing ZhangLing ZhangLing ZhangLeichang PanLeichang PanFrontiers Media S.A.articleduck plague virusLORF5 genevirus replicationnon-essentialcell-to-cell spreadMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021) |
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duck plague virus LORF5 gene virus replication non-essential cell-to-cell spread Microbiology QR1-502 |
| spellingShingle |
duck plague virus LORF5 gene virus replication non-essential cell-to-cell spread Microbiology QR1-502 Bingjie Shen Bingjie Shen Bingjie Shen Yunjiao Li Yunjiao Li Yunjiao Li Anchun Cheng Anchun Cheng Anchun Cheng Mingshu Wang Mingshu Wang Mingshu Wang Ying Wu Ying Wu Ying Wu Qiao Yang Qiao Yang Qiao Yang Renyong Jia Renyong Jia Renyong Jia Bin Tian Bin Tian Bin Tian Xumin Ou Xumin Ou Xumin Ou Sai Mao Sai Mao Sai Mao Di Sun Di Sun Di Sun Shaqiu Zhang Shaqiu Zhang Shaqiu Zhang Dekang Zhu Dekang Zhu Shun Chen Shun Chen Shun Chen Mafeng Liu Mafeng Liu Mafeng Liu Xin-Xin Zhao Xin-Xin Zhao Xin-Xin Zhao Juan Huang Juan Huang Juan Huang Qun Gao Qun Gao Qun Gao Yunya Liu Yunya Liu Yunya Liu Yanling Yu Yanling Yu Yanling Yu Ling Zhang Ling Zhang Ling Zhang Leichang Pan Leichang Pan The LORF5 Gene Is Non-essential for Replication but Important for Duck Plague Virus Cell-to-Cell Spread Efficiently in Host Cells |
| description |
Duck plague virus (DPV) can cause high morbidity and mortality in many waterfowl species within the order Anseriformes. The DPV genome contains 78 open reading frames (ORFs), among which the LORF2, LORF3, LORF4, LORF5, and SORF3 genes are unique genes of avian herpesvirus. In this study, to investigate the role of this unique LORF5 gene in DPV proliferation, we generated a recombinant virus that lacks the LORF5 gene by a two-step red recombination system, which cloned the DPV Chinese virulent strain (DPV CHv) genome into a bacterial artificial chromosome (DPV CHv-BAC); the proliferation law of LORF5-deleted mutant virus on DEF cells and the effect of LORF5 gene on the life cycle stages of DPV compared with the parent strain were tested. Our data revealed that the LORF5 gene contributes to the cell-to-cell transmission of DPV but is not relevant to virus invasion, replication, assembly, and release formation. Taken together, this study sheds light on the role of the avian herpesvirus-specific gene LORF5 in the DPV proliferation life cycle. These findings lay the foundation for in-depth functional studies of the LORF5 gene in DPV or other avian herpesviruses. |
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| author |
Bingjie Shen Bingjie Shen Bingjie Shen Yunjiao Li Yunjiao Li Yunjiao Li Anchun Cheng Anchun Cheng Anchun Cheng Mingshu Wang Mingshu Wang Mingshu Wang Ying Wu Ying Wu Ying Wu Qiao Yang Qiao Yang Qiao Yang Renyong Jia Renyong Jia Renyong Jia Bin Tian Bin Tian Bin Tian Xumin Ou Xumin Ou Xumin Ou Sai Mao Sai Mao Sai Mao Di Sun Di Sun Di Sun Shaqiu Zhang Shaqiu Zhang Shaqiu Zhang Dekang Zhu Dekang Zhu Shun Chen Shun Chen Shun Chen Mafeng Liu Mafeng Liu Mafeng Liu Xin-Xin Zhao Xin-Xin Zhao Xin-Xin Zhao Juan Huang Juan Huang Juan Huang Qun Gao Qun Gao Qun Gao Yunya Liu Yunya Liu Yunya Liu Yanling Yu Yanling Yu Yanling Yu Ling Zhang Ling Zhang Ling Zhang Leichang Pan Leichang Pan |
| author_facet |
Bingjie Shen Bingjie Shen Bingjie Shen Yunjiao Li Yunjiao Li Yunjiao Li Anchun Cheng Anchun Cheng Anchun Cheng Mingshu Wang Mingshu Wang Mingshu Wang Ying Wu Ying Wu Ying Wu Qiao Yang Qiao Yang Qiao Yang Renyong Jia Renyong Jia Renyong Jia Bin Tian Bin Tian Bin Tian Xumin Ou Xumin Ou Xumin Ou Sai Mao Sai Mao Sai Mao Di Sun Di Sun Di Sun Shaqiu Zhang Shaqiu Zhang Shaqiu Zhang Dekang Zhu Dekang Zhu Shun Chen Shun Chen Shun Chen Mafeng Liu Mafeng Liu Mafeng Liu Xin-Xin Zhao Xin-Xin Zhao Xin-Xin Zhao Juan Huang Juan Huang Juan Huang Qun Gao Qun Gao Qun Gao Yunya Liu Yunya Liu Yunya Liu Yanling Yu Yanling Yu Yanling Yu Ling Zhang Ling Zhang Ling Zhang Leichang Pan Leichang Pan |
| author_sort |
Bingjie Shen |
| title |
The LORF5 Gene Is Non-essential for Replication but Important for Duck Plague Virus Cell-to-Cell Spread Efficiently in Host Cells |
| title_short |
The LORF5 Gene Is Non-essential for Replication but Important for Duck Plague Virus Cell-to-Cell Spread Efficiently in Host Cells |
| title_full |
The LORF5 Gene Is Non-essential for Replication but Important for Duck Plague Virus Cell-to-Cell Spread Efficiently in Host Cells |
| title_fullStr |
The LORF5 Gene Is Non-essential for Replication but Important for Duck Plague Virus Cell-to-Cell Spread Efficiently in Host Cells |
| title_full_unstemmed |
The LORF5 Gene Is Non-essential for Replication but Important for Duck Plague Virus Cell-to-Cell Spread Efficiently in Host Cells |
| title_sort |
lorf5 gene is non-essential for replication but important for duck plague virus cell-to-cell spread efficiently in host cells |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/1861102915e94b71bee30d074dd1165e |
| work_keys_str_mv |
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