Curcumin mediates repulsive guidance molecule B (RGMb) in the treatment mechanism of renal fibrosis induced by unilateral ureteral obstruction

In this study, we explored the role and mechanism of repulsive guidance molecule B (RGMb, also known as Dragon) in the protective effects of curcumin against renal fibrosis and verified Dragon’s effect on renal tubular epithelial cell apoptosis and cell programmability. Unilateral ureteral obstructi...

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Autores principales:	Fei Chen, Yu Xie, Qin Lv, Wei Zou, Liyan Xiong
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	curcumin dragon renal fibrosis unilateral ureteral obstruction repulsive guidance molecule b Diseases of the genitourinary system. Urology RC870-923
Acceso en línea:	https://doaj.org/article/1865372fb72a4019b97e9b26dc69b358
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spelling	oai:doaj.org-article:1865372fb72a4019b97e9b26dc69b3582021-11-11T14:23:40ZCurcumin mediates repulsive guidance molecule B (RGMb) in the treatment mechanism of renal fibrosis induced by unilateral ureteral obstruction0886-022X1525-604910.1080/0886022X.2021.1997764https://doaj.org/article/1865372fb72a4019b97e9b26dc69b3582021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/0886022X.2021.1997764https://doaj.org/toc/0886-022Xhttps://doaj.org/toc/1525-6049In this study, we explored the role and mechanism of repulsive guidance molecule B (RGMb, also known as Dragon) in the protective effects of curcumin against renal fibrosis and verified Dragon’s effect on renal tubular epithelial cell apoptosis and cell programmability. Unilateral ureteral obstruction (UUO) was surgically induced in rats to establish a model of renal interstitial fibrosis (RIF). The rats were then treated with curcumin. Curcumin prominently decreased the serum creatinine (SCr) and blood urea nitrogen (BUN) levels, and also improved the tubular injury in the UUO-induced rats. Curcumin significantly downregulated the TGF-β1, P-Smad2/3, cleaved caspase-3, cleaved caspase-8 and Dragon levels. Dragon knockdown also markedly reduced the TGF-β1, P-Smad2/3, Smad2/3, cleaved caspase-3, cleaved caspase-8, fibronectin, collagen I, collagen IV, vimentin, and α-SMA expression levels. Conversely, Dragon overexpression caused higher expression levels of these proteins, and curcumin reversed this effect. Furthermore, Dragon knockdown increased the E-cadherin levels, whereas Dragon overexpression decreased these levels. Overexpressing Dragon significantly decreased the cell viability, and curcumin reversed this effect. In conclusion, curcumin acted on Dragon and attenuated RIF in UUO rat models. Curcumin downregulated the TGF-β1/Smad signaling pathway and inhibited Dragon and fibrogenic molecules in both rats and HK-2 cells.Fei ChenYu XieQin LvWei ZouLiyan XiongTaylor & Francis Grouparticlecurcumindragonrenal fibrosisunilateral ureteral obstructionrepulsive guidance molecule bDiseases of the genitourinary system. UrologyRC870-923ENRenal Failure, Vol 43, Iss 1, Pp 1496-1505 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	curcumin dragon renal fibrosis unilateral ureteral obstruction repulsive guidance molecule b Diseases of the genitourinary system. Urology RC870-923
spellingShingle	curcumin dragon renal fibrosis unilateral ureteral obstruction repulsive guidance molecule b Diseases of the genitourinary system. Urology RC870-923 Fei Chen Yu Xie Qin Lv Wei Zou Liyan Xiong Curcumin mediates repulsive guidance molecule B (RGMb) in the treatment mechanism of renal fibrosis induced by unilateral ureteral obstruction
description	In this study, we explored the role and mechanism of repulsive guidance molecule B (RGMb, also known as Dragon) in the protective effects of curcumin against renal fibrosis and verified Dragon’s effect on renal tubular epithelial cell apoptosis and cell programmability. Unilateral ureteral obstruction (UUO) was surgically induced in rats to establish a model of renal interstitial fibrosis (RIF). The rats were then treated with curcumin. Curcumin prominently decreased the serum creatinine (SCr) and blood urea nitrogen (BUN) levels, and also improved the tubular injury in the UUO-induced rats. Curcumin significantly downregulated the TGF-β1, P-Smad2/3, cleaved caspase-3, cleaved caspase-8 and Dragon levels. Dragon knockdown also markedly reduced the TGF-β1, P-Smad2/3, Smad2/3, cleaved caspase-3, cleaved caspase-8, fibronectin, collagen I, collagen IV, vimentin, and α-SMA expression levels. Conversely, Dragon overexpression caused higher expression levels of these proteins, and curcumin reversed this effect. Furthermore, Dragon knockdown increased the E-cadherin levels, whereas Dragon overexpression decreased these levels. Overexpressing Dragon significantly decreased the cell viability, and curcumin reversed this effect. In conclusion, curcumin acted on Dragon and attenuated RIF in UUO rat models. Curcumin downregulated the TGF-β1/Smad signaling pathway and inhibited Dragon and fibrogenic molecules in both rats and HK-2 cells.
format	article
author	Fei Chen Yu Xie Qin Lv Wei Zou Liyan Xiong
author_facet	Fei Chen Yu Xie Qin Lv Wei Zou Liyan Xiong
author_sort	Fei Chen
title	Curcumin mediates repulsive guidance molecule B (RGMb) in the treatment mechanism of renal fibrosis induced by unilateral ureteral obstruction
title_short	Curcumin mediates repulsive guidance molecule B (RGMb) in the treatment mechanism of renal fibrosis induced by unilateral ureteral obstruction
title_full	Curcumin mediates repulsive guidance molecule B (RGMb) in the treatment mechanism of renal fibrosis induced by unilateral ureteral obstruction
title_fullStr	Curcumin mediates repulsive guidance molecule B (RGMb) in the treatment mechanism of renal fibrosis induced by unilateral ureteral obstruction
title_full_unstemmed	Curcumin mediates repulsive guidance molecule B (RGMb) in the treatment mechanism of renal fibrosis induced by unilateral ureteral obstruction
title_sort	curcumin mediates repulsive guidance molecule b (rgmb) in the treatment mechanism of renal fibrosis induced by unilateral ureteral obstruction
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/1865372fb72a4019b97e9b26dc69b358
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Curcumin mediates repulsive guidance molecule B (RGMb) in the treatment mechanism of renal fibrosis induced by unilateral ureteral obstruction

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