Mutations introduced in susceptibility genes through CRISPR/Cas9 genome editing confer increased late blight resistance in potatoes

Abstract The use of pathogen-resistant cultivars is expected to increase yield and decrease fungicide use in agriculture. However, in potato breeding, increased resistance obtained via resistance genes (R-genes) is hampered because R-gene(s) are often specific for a pathogen race and can be quickly...

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Autores principales:	Nam Phuong Kieu, Marit Lenman, Eu Sheng Wang, Bent Larsen Petersen, Erik Andreasson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/186950fad1c14afebbab75569993fce1
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spelling	oai:doaj.org-article:186950fad1c14afebbab75569993fce12021-12-02T13:34:46ZMutations introduced in susceptibility genes through CRISPR/Cas9 genome editing confer increased late blight resistance in potatoes10.1038/s41598-021-83972-w2045-2322https://doaj.org/article/186950fad1c14afebbab75569993fce12021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83972-whttps://doaj.org/toc/2045-2322Abstract The use of pathogen-resistant cultivars is expected to increase yield and decrease fungicide use in agriculture. However, in potato breeding, increased resistance obtained via resistance genes (R-genes) is hampered because R-gene(s) are often specific for a pathogen race and can be quickly overcome by the evolution of the pathogen. In parallel, susceptibility genes (S-genes) are important for pathogenesis, and loss of S-gene function confers increased resistance in several plants, such as rice, wheat, citrus and tomatoes. In this article, we present the mutation and screening of seven putative S-genes in potatoes, including two DMR6 potato homologues. Using a CRISPR/Cas9 system, which conferred co-expression of two guide RNAs, tetra-allelic deletion mutants were generated and resistance against late blight was assayed in the plants. Functional knockouts of StDND1, StCHL1, and DMG400000582 (StDMR6-1) generated potatoes with increased resistance against late blight. Plants mutated in StDND1 showed pleiotropic effects, whereas StDMR6-1 and StCHL1 mutated plants did not exhibit any growth phenotype, making them good candidates for further agricultural studies. Additionally, we showed that DMG401026923 (here denoted StDMR6-2) knockout mutants did not demonstrate any increased late blight resistance, but exhibited a growth phenotype, indicating that StDMR6-1 and StDMR6-2 have different functions. To the best of our knowledge, this is the first report on the mutation and screening of putative S-genes in potatoes, including two DMR6 potato homologues.Nam Phuong KieuMarit LenmanEu Sheng WangBent Larsen PetersenErik AndreassonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nam Phuong Kieu Marit Lenman Eu Sheng Wang Bent Larsen Petersen Erik Andreasson Mutations introduced in susceptibility genes through CRISPR/Cas9 genome editing confer increased late blight resistance in potatoes
description	Abstract The use of pathogen-resistant cultivars is expected to increase yield and decrease fungicide use in agriculture. However, in potato breeding, increased resistance obtained via resistance genes (R-genes) is hampered because R-gene(s) are often specific for a pathogen race and can be quickly overcome by the evolution of the pathogen. In parallel, susceptibility genes (S-genes) are important for pathogenesis, and loss of S-gene function confers increased resistance in several plants, such as rice, wheat, citrus and tomatoes. In this article, we present the mutation and screening of seven putative S-genes in potatoes, including two DMR6 potato homologues. Using a CRISPR/Cas9 system, which conferred co-expression of two guide RNAs, tetra-allelic deletion mutants were generated and resistance against late blight was assayed in the plants. Functional knockouts of StDND1, StCHL1, and DMG400000582 (StDMR6-1) generated potatoes with increased resistance against late blight. Plants mutated in StDND1 showed pleiotropic effects, whereas StDMR6-1 and StCHL1 mutated plants did not exhibit any growth phenotype, making them good candidates for further agricultural studies. Additionally, we showed that DMG401026923 (here denoted StDMR6-2) knockout mutants did not demonstrate any increased late blight resistance, but exhibited a growth phenotype, indicating that StDMR6-1 and StDMR6-2 have different functions. To the best of our knowledge, this is the first report on the mutation and screening of putative S-genes in potatoes, including two DMR6 potato homologues.
format	article
author	Nam Phuong Kieu Marit Lenman Eu Sheng Wang Bent Larsen Petersen Erik Andreasson
author_facet	Nam Phuong Kieu Marit Lenman Eu Sheng Wang Bent Larsen Petersen Erik Andreasson
author_sort	Nam Phuong Kieu
title	Mutations introduced in susceptibility genes through CRISPR/Cas9 genome editing confer increased late blight resistance in potatoes
title_short	Mutations introduced in susceptibility genes through CRISPR/Cas9 genome editing confer increased late blight resistance in potatoes
title_full	Mutations introduced in susceptibility genes through CRISPR/Cas9 genome editing confer increased late blight resistance in potatoes
title_fullStr	Mutations introduced in susceptibility genes through CRISPR/Cas9 genome editing confer increased late blight resistance in potatoes
title_full_unstemmed	Mutations introduced in susceptibility genes through CRISPR/Cas9 genome editing confer increased late blight resistance in potatoes
title_sort	mutations introduced in susceptibility genes through crispr/cas9 genome editing confer increased late blight resistance in potatoes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/186950fad1c14afebbab75569993fce1
work_keys_str_mv	AT namphuongkieu mutationsintroducedinsusceptibilitygenesthroughcrisprcas9genomeeditingconferincreasedlateblightresistanceinpotatoes AT maritlenman mutationsintroducedinsusceptibilitygenesthroughcrisprcas9genomeeditingconferincreasedlateblightresistanceinpotatoes AT eushengwang mutationsintroducedinsusceptibilitygenesthroughcrisprcas9genomeeditingconferincreasedlateblightresistanceinpotatoes AT bentlarsenpetersen mutationsintroducedinsusceptibilitygenesthroughcrisprcas9genomeeditingconferincreasedlateblightresistanceinpotatoes AT erikandreasson mutationsintroducedinsusceptibilitygenesthroughcrisprcas9genomeeditingconferincreasedlateblightresistanceinpotatoes
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Mutations introduced in susceptibility genes through CRISPR/Cas9 genome editing confer increased late blight resistance in potatoes

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