A simple and rapid diagnostic method for 13 types of high-risk human papillomavirus (HR-HPV) detection using CRISPR-Cas12a technology

A simple and rapid diagnostic method for 13 types of high-risk human papillomavirus (HR-HPV) detection using CRISPR-Cas12a technology

Abstract Cervical carcinoma is the second most common cancer in women worldwide with greater than 99% of the cases caused by human papillomaviruses (HPVs). Early detection of HPVs especially the high risk types (HR-HPVs) are essential to prevent the disease progression. The existing methods for HPV...

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Autores principales: Jiaojiao Gong, Guanghui Zhang, Wangguo Wang, Liping Liang, Qianyun Li, Menghao Liu, Liang Xue, Guanghui Tang
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Science
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Acceso en línea:https://doaj.org/article/187295dff3134ec8935b1df1dfef0400
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spelling oai:doaj.org-article:187295dff3134ec8935b1df1dfef04002021-12-02T17:40:45ZA simple and rapid diagnostic method for 13 types of high-risk human papillomavirus (HR-HPV) detection using CRISPR-Cas12a technology10.1038/s41598-021-92329-22045-2322https://doaj.org/article/187295dff3134ec8935b1df1dfef04002021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92329-2https://doaj.org/toc/2045-2322Abstract Cervical carcinoma is the second most common cancer in women worldwide with greater than 99% of the cases caused by human papillomaviruses (HPVs). Early detection of HPVs especially the high risk types (HR-HPVs) are essential to prevent the disease progression. The existing methods for HPV detection, such as qPCR are of high sensitivity and specificity, but the need for expensive machinery and well-trained personnel slow down the disease detection. The emerging Cas12a-based method presents a new technique for nucleic acid detection. However, it is time-consuming and labor-intensive when used for HPV detection, as several reactions are required in order to identify multiple HPV infections. We herein present a non-genotyping method for 13 types of HR-HPV detection in a single reaction by combining the isothermal recombinase polymerase amplification (RPA) method with CRISPR-Cas12a technology. The result could be achieved in 35 min with high sensitivity (500 copies per reaction). This assay represents great advances for the application of RPA-Cas12a system and holds a great potential to address the key challenges facing the HPV diagnostics.Jiaojiao GongGuanghui ZhangWangguo WangLiping LiangQianyun LiMenghao LiuLiang XueGuanghui TangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Jiaojiao Gong
Guanghui Zhang
Wangguo Wang
Liping Liang
Qianyun Li
Menghao Liu
Liang Xue
Guanghui Tang
A simple and rapid diagnostic method for 13 types of high-risk human papillomavirus (HR-HPV) detection using CRISPR-Cas12a technology
description Abstract Cervical carcinoma is the second most common cancer in women worldwide with greater than 99% of the cases caused by human papillomaviruses (HPVs). Early detection of HPVs especially the high risk types (HR-HPVs) are essential to prevent the disease progression. The existing methods for HPV detection, such as qPCR are of high sensitivity and specificity, but the need for expensive machinery and well-trained personnel slow down the disease detection. The emerging Cas12a-based method presents a new technique for nucleic acid detection. However, it is time-consuming and labor-intensive when used for HPV detection, as several reactions are required in order to identify multiple HPV infections. We herein present a non-genotyping method for 13 types of HR-HPV detection in a single reaction by combining the isothermal recombinase polymerase amplification (RPA) method with CRISPR-Cas12a technology. The result could be achieved in 35 min with high sensitivity (500 copies per reaction). This assay represents great advances for the application of RPA-Cas12a system and holds a great potential to address the key challenges facing the HPV diagnostics.
format article
author Jiaojiao Gong
Guanghui Zhang
Wangguo Wang
Liping Liang
Qianyun Li
Menghao Liu
Liang Xue
Guanghui Tang
author_facet Jiaojiao Gong
Guanghui Zhang
Wangguo Wang
Liping Liang
Qianyun Li
Menghao Liu
Liang Xue
Guanghui Tang
author_sort Jiaojiao Gong
title A simple and rapid diagnostic method for 13 types of high-risk human papillomavirus (HR-HPV) detection using CRISPR-Cas12a technology
title_short A simple and rapid diagnostic method for 13 types of high-risk human papillomavirus (HR-HPV) detection using CRISPR-Cas12a technology
title_full A simple and rapid diagnostic method for 13 types of high-risk human papillomavirus (HR-HPV) detection using CRISPR-Cas12a technology
title_fullStr A simple and rapid diagnostic method for 13 types of high-risk human papillomavirus (HR-HPV) detection using CRISPR-Cas12a technology
title_full_unstemmed A simple and rapid diagnostic method for 13 types of high-risk human papillomavirus (HR-HPV) detection using CRISPR-Cas12a technology
title_sort simple and rapid diagnostic method for 13 types of high-risk human papillomavirus (hr-hpv) detection using crispr-cas12a technology
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/187295dff3134ec8935b1df1dfef0400
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