Mechanical activities of self-beating cardiomyocyte aggregates under mechanical compression

Abstract Since the discovery of synchronous pulsations in cardiomyocytes (CMs), electrical communication between CMs has been emphasized; however, recent studies suggest the possibility of mechanical communication. Here, we demonstrate that spherical self-beating CM aggregates, termed cardiac sphero...

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Autores principales:	Ken Nakano, Naoya Nanri, Yoshinari Tsukamoto, Mitsuru Akashi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1874f27ea7d04c86b505e225c69db5b8
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spelling	oai:doaj.org-article:1874f27ea7d04c86b505e225c69db5b82021-12-02T18:47:10ZMechanical activities of self-beating cardiomyocyte aggregates under mechanical compression10.1038/s41598-021-93657-z2045-2322https://doaj.org/article/1874f27ea7d04c86b505e225c69db5b82021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93657-zhttps://doaj.org/toc/2045-2322Abstract Since the discovery of synchronous pulsations in cardiomyocytes (CMs), electrical communication between CMs has been emphasized; however, recent studies suggest the possibility of mechanical communication. Here, we demonstrate that spherical self-beating CM aggregates, termed cardiac spheroids (CSs), produce enhanced mechanical energy under mechanical compression and work cooperatively via mechanical communication. For single CSs between parallel plates, compression increased both beating frequency and beating energy. Contact mechanics revealed a scaling law on the beating energy, indicating that the most intensively stressed cells in the compressed CSs predominantly contributed to the performance of mechanical work against mechanical compression. For pairs of CSs between parallel plates, compression immediately caused synchronous beating with mechanical coupling. Compression tended to strengthen and stabilize the synchronous beating, although some irregularity and temporary arrest were observed. These results suggest that mechanical compression is an indispensable control parameter when evaluating the activities of CMs and their aggregates.Ken NakanoNaoya NanriYoshinari TsukamotoMitsuru AkashiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ken Nakano Naoya Nanri Yoshinari Tsukamoto Mitsuru Akashi Mechanical activities of self-beating cardiomyocyte aggregates under mechanical compression
description	Abstract Since the discovery of synchronous pulsations in cardiomyocytes (CMs), electrical communication between CMs has been emphasized; however, recent studies suggest the possibility of mechanical communication. Here, we demonstrate that spherical self-beating CM aggregates, termed cardiac spheroids (CSs), produce enhanced mechanical energy under mechanical compression and work cooperatively via mechanical communication. For single CSs between parallel plates, compression increased both beating frequency and beating energy. Contact mechanics revealed a scaling law on the beating energy, indicating that the most intensively stressed cells in the compressed CSs predominantly contributed to the performance of mechanical work against mechanical compression. For pairs of CSs between parallel plates, compression immediately caused synchronous beating with mechanical coupling. Compression tended to strengthen and stabilize the synchronous beating, although some irregularity and temporary arrest were observed. These results suggest that mechanical compression is an indispensable control parameter when evaluating the activities of CMs and their aggregates.
format	article
author	Ken Nakano Naoya Nanri Yoshinari Tsukamoto Mitsuru Akashi
author_facet	Ken Nakano Naoya Nanri Yoshinari Tsukamoto Mitsuru Akashi
author_sort	Ken Nakano
title	Mechanical activities of self-beating cardiomyocyte aggregates under mechanical compression
title_short	Mechanical activities of self-beating cardiomyocyte aggregates under mechanical compression
title_full	Mechanical activities of self-beating cardiomyocyte aggregates under mechanical compression
title_fullStr	Mechanical activities of self-beating cardiomyocyte aggregates under mechanical compression
title_full_unstemmed	Mechanical activities of self-beating cardiomyocyte aggregates under mechanical compression
title_sort	mechanical activities of self-beating cardiomyocyte aggregates under mechanical compression
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1874f27ea7d04c86b505e225c69db5b8
work_keys_str_mv	AT kennakano mechanicalactivitiesofselfbeatingcardiomyocyteaggregatesundermechanicalcompression AT naoyananri mechanicalactivitiesofselfbeatingcardiomyocyteaggregatesundermechanicalcompression AT yoshinaritsukamoto mechanicalactivitiesofselfbeatingcardiomyocyteaggregatesundermechanicalcompression AT mitsuruakashi mechanicalactivitiesofselfbeatingcardiomyocyteaggregatesundermechanicalcompression
_version_	1718377607462912000

Mechanical activities of self-beating cardiomyocyte aggregates under mechanical compression

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