Identification of novel sRNAs in mycobacterial species.

Bacterial small RNAs (sRNAs) are short transcripts that typically do not encode proteins and often act as regulators of gene expression through a variety of mechanisms. Regulatory sRNAs have been identified in many species, including Mycobacterium tuberculosis, the causative agent of tuberculosis. H...

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Autores principales:	Chen-Hsun Tsai, Catherine Baranowski, Jonathan Livny, Kathleen A McDonough, Joseph T Wade, Lydia M Contreras
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/18758fc2f0764dbaa8bf7a4b02ef57fa
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spelling	oai:doaj.org-article:18758fc2f0764dbaa8bf7a4b02ef57fa2021-11-18T08:46:31ZIdentification of novel sRNAs in mycobacterial species.1932-620310.1371/journal.pone.0079411https://doaj.org/article/18758fc2f0764dbaa8bf7a4b02ef57fa2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24244498/?tool=EBIhttps://doaj.org/toc/1932-6203Bacterial small RNAs (sRNAs) are short transcripts that typically do not encode proteins and often act as regulators of gene expression through a variety of mechanisms. Regulatory sRNAs have been identified in many species, including Mycobacterium tuberculosis, the causative agent of tuberculosis. Here, we use a computational algorithm to predict sRNA candidates in the mycobacterial species M. smegmatis and M. bovis BCG and confirmed the expression of many sRNAs using Northern blotting. Thus, we have identified 17 and 23 novel sRNAs in M. smegmatis and M. bovis BCG, respectively. We have also applied a high-throughput technique (Deep-RACE) to map the 5' and 3' ends of many of these sRNAs and identified potential regulators of sRNAs by analysis of existing ChIP-seq datasets. The sRNAs identified in this work likely contribute to the unique biology of mycobacteria.Chen-Hsun TsaiCatherine BaranowskiJonathan LivnyKathleen A McDonoughJoseph T WadeLydia M ContrerasPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e79411 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chen-Hsun Tsai Catherine Baranowski Jonathan Livny Kathleen A McDonough Joseph T Wade Lydia M Contreras Identification of novel sRNAs in mycobacterial species.
description	Bacterial small RNAs (sRNAs) are short transcripts that typically do not encode proteins and often act as regulators of gene expression through a variety of mechanisms. Regulatory sRNAs have been identified in many species, including Mycobacterium tuberculosis, the causative agent of tuberculosis. Here, we use a computational algorithm to predict sRNA candidates in the mycobacterial species M. smegmatis and M. bovis BCG and confirmed the expression of many sRNAs using Northern blotting. Thus, we have identified 17 and 23 novel sRNAs in M. smegmatis and M. bovis BCG, respectively. We have also applied a high-throughput technique (Deep-RACE) to map the 5' and 3' ends of many of these sRNAs and identified potential regulators of sRNAs by analysis of existing ChIP-seq datasets. The sRNAs identified in this work likely contribute to the unique biology of mycobacteria.
format	article
author	Chen-Hsun Tsai Catherine Baranowski Jonathan Livny Kathleen A McDonough Joseph T Wade Lydia M Contreras
author_facet	Chen-Hsun Tsai Catherine Baranowski Jonathan Livny Kathleen A McDonough Joseph T Wade Lydia M Contreras
author_sort	Chen-Hsun Tsai
title	Identification of novel sRNAs in mycobacterial species.
title_short	Identification of novel sRNAs in mycobacterial species.
title_full	Identification of novel sRNAs in mycobacterial species.
title_fullStr	Identification of novel sRNAs in mycobacterial species.
title_full_unstemmed	Identification of novel sRNAs in mycobacterial species.
title_sort	identification of novel srnas in mycobacterial species.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/18758fc2f0764dbaa8bf7a4b02ef57fa
work_keys_str_mv	AT chenhsuntsai identificationofnovelsrnasinmycobacterialspecies AT catherinebaranowski identificationofnovelsrnasinmycobacterialspecies AT jonathanlivny identificationofnovelsrnasinmycobacterialspecies AT kathleenamcdonough identificationofnovelsrnasinmycobacterialspecies AT josephtwade identificationofnovelsrnasinmycobacterialspecies AT lydiamcontreras identificationofnovelsrnasinmycobacterialspecies
_version_	1718421307549286400

Identification of novel sRNAs in mycobacterial species.

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