Interleukin-6/STAT3 signalling regulates adipocyte induced epithelial-mesenchymal transition in breast cancer cells

Abstract The tumour microenvironment is a key regulators of tumour progression through the secretion of growth factors that activate epithelial-mesenchymal transition (EMT). Induction of EMT is a key step for transition from a benign state to a metastatic tumour. Adipose tissue forms a bulk portion...

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Autores principales:	Jones Gyamfi, Yun-Hee Lee, Minseob Eom, Junjeong Choi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/187b74812dc443519a146de4eba5ee15
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spelling	oai:doaj.org-article:187b74812dc443519a146de4eba5ee152021-12-02T15:08:00ZInterleukin-6/STAT3 signalling regulates adipocyte induced epithelial-mesenchymal transition in breast cancer cells10.1038/s41598-018-27184-92045-2322https://doaj.org/article/187b74812dc443519a146de4eba5ee152018-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-27184-9https://doaj.org/toc/2045-2322Abstract The tumour microenvironment is a key regulators of tumour progression through the secretion of growth factors that activate epithelial-mesenchymal transition (EMT). Induction of EMT is a key step for transition from a benign state to a metastatic tumour. Adipose tissue forms a bulk portion of the breast cancer microenvironment, emerging evidence indicates the potential for adipocytes to influence tumour progression through the secretion of adipokines that can induce EMT. The molecular mechanisms underlying how adipocytes enhance breast cancer progression is largely unknown. We hypothesized that paracrine signalling by adipocytes can activate EMT and results in increased migration and invasion characteristics of breast cancer cells. We found that IL-6 secreted by adipocytes induce EMT in breast cancer cells. The effect of IL-6 expression on EMT is mediated through activation of the signal transducer and activated of transcription 3 (STAT3). Blocking of IL-6 signalling in breast cancer cells and adipocytes, decreased proliferation, migration and invasion capabilities and altered the expression of genes regulating EMT. Together, our results suggest that matured human adipocytes can enhance the aggressive behaviour of breast cancer cells and induce an EMT-phenotype through paracrine IL-6/STAT3 signalling.Jones GyamfiYun-Hee LeeMinseob EomJunjeong ChoiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jones Gyamfi Yun-Hee Lee Minseob Eom Junjeong Choi Interleukin-6/STAT3 signalling regulates adipocyte induced epithelial-mesenchymal transition in breast cancer cells
description	Abstract The tumour microenvironment is a key regulators of tumour progression through the secretion of growth factors that activate epithelial-mesenchymal transition (EMT). Induction of EMT is a key step for transition from a benign state to a metastatic tumour. Adipose tissue forms a bulk portion of the breast cancer microenvironment, emerging evidence indicates the potential for adipocytes to influence tumour progression through the secretion of adipokines that can induce EMT. The molecular mechanisms underlying how adipocytes enhance breast cancer progression is largely unknown. We hypothesized that paracrine signalling by adipocytes can activate EMT and results in increased migration and invasion characteristics of breast cancer cells. We found that IL-6 secreted by adipocytes induce EMT in breast cancer cells. The effect of IL-6 expression on EMT is mediated through activation of the signal transducer and activated of transcription 3 (STAT3). Blocking of IL-6 signalling in breast cancer cells and adipocytes, decreased proliferation, migration and invasion capabilities and altered the expression of genes regulating EMT. Together, our results suggest that matured human adipocytes can enhance the aggressive behaviour of breast cancer cells and induce an EMT-phenotype through paracrine IL-6/STAT3 signalling.
format	article
author	Jones Gyamfi Yun-Hee Lee Minseob Eom Junjeong Choi
author_facet	Jones Gyamfi Yun-Hee Lee Minseob Eom Junjeong Choi
author_sort	Jones Gyamfi
title	Interleukin-6/STAT3 signalling regulates adipocyte induced epithelial-mesenchymal transition in breast cancer cells
title_short	Interleukin-6/STAT3 signalling regulates adipocyte induced epithelial-mesenchymal transition in breast cancer cells
title_full	Interleukin-6/STAT3 signalling regulates adipocyte induced epithelial-mesenchymal transition in breast cancer cells
title_fullStr	Interleukin-6/STAT3 signalling regulates adipocyte induced epithelial-mesenchymal transition in breast cancer cells
title_full_unstemmed	Interleukin-6/STAT3 signalling regulates adipocyte induced epithelial-mesenchymal transition in breast cancer cells
title_sort	interleukin-6/stat3 signalling regulates adipocyte induced epithelial-mesenchymal transition in breast cancer cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/187b74812dc443519a146de4eba5ee15
work_keys_str_mv	AT jonesgyamfi interleukin6stat3signallingregulatesadipocyteinducedepithelialmesenchymaltransitioninbreastcancercells AT yunheelee interleukin6stat3signallingregulatesadipocyteinducedepithelialmesenchymaltransitioninbreastcancercells AT minseobeom interleukin6stat3signallingregulatesadipocyteinducedepithelialmesenchymaltransitioninbreastcancercells AT junjeongchoi interleukin6stat3signallingregulatesadipocyteinducedepithelialmesenchymaltransitioninbreastcancercells
_version_	1718388301750075392

Interleukin-6/STAT3 signalling regulates adipocyte induced epithelial-mesenchymal transition in breast cancer cells

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