Possible involvement of cone opsins in distinct photoresponses of intrinsically photosensitive dermal chromatophores in tilapia Oreochromis niloticus.

Dermal specialized pigment cells (chromatophores) are thought to be one type of extraretinal photoreceptors responsible for a wide variety of sensory tasks, including adjusting body coloration. Unlike the well-studied image-forming function in retinal photoreceptors, direct evidence characterizing t...

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Autores principales:	Shyh-Chi Chen, R Meldrum Robertson, Craig W Hawryshyn
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1886790e2f2841f69f13647b8f951eb1
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spelling	oai:doaj.org-article:1886790e2f2841f69f13647b8f951eb12021-11-18T09:01:23ZPossible involvement of cone opsins in distinct photoresponses of intrinsically photosensitive dermal chromatophores in tilapia Oreochromis niloticus.1932-620310.1371/journal.pone.0070342https://doaj.org/article/1886790e2f2841f69f13647b8f951eb12013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23940562/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Dermal specialized pigment cells (chromatophores) are thought to be one type of extraretinal photoreceptors responsible for a wide variety of sensory tasks, including adjusting body coloration. Unlike the well-studied image-forming function in retinal photoreceptors, direct evidence characterizing the mechanism of chromatophore photoresponses is less understood, particularly at the molecular and cellular levels. In the present study, cone opsin expression was detected in tilapia caudal fin where photosensitive chromatophores exist. Single-cell RT-PCR revealed co-existence of different cone opsins within melanophores and erythrophores. By stimulating cells with six wavelengths ranging from 380 to 580 nm, we found melanophores and erythrophores showed distinct photoresponses. After exposed to light, regardless of wavelength presentation, melanophores dispersed and maintained cell shape in an expansion stage by shuttling pigment granules. Conversely, erythrophores aggregated or dispersed pigment granules when exposed to short- or middle/long-wavelength light, respectively. These results suggest that diverse molecular mechanisms and light-detecting strategies may be employed by different types of tilapia chromatophores, which are instrumental in pigment pattern formation.Shyh-Chi ChenR Meldrum RobertsonCraig W HawryshynPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e70342 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shyh-Chi Chen R Meldrum Robertson Craig W Hawryshyn Possible involvement of cone opsins in distinct photoresponses of intrinsically photosensitive dermal chromatophores in tilapia Oreochromis niloticus.
description	Dermal specialized pigment cells (chromatophores) are thought to be one type of extraretinal photoreceptors responsible for a wide variety of sensory tasks, including adjusting body coloration. Unlike the well-studied image-forming function in retinal photoreceptors, direct evidence characterizing the mechanism of chromatophore photoresponses is less understood, particularly at the molecular and cellular levels. In the present study, cone opsin expression was detected in tilapia caudal fin where photosensitive chromatophores exist. Single-cell RT-PCR revealed co-existence of different cone opsins within melanophores and erythrophores. By stimulating cells with six wavelengths ranging from 380 to 580 nm, we found melanophores and erythrophores showed distinct photoresponses. After exposed to light, regardless of wavelength presentation, melanophores dispersed and maintained cell shape in an expansion stage by shuttling pigment granules. Conversely, erythrophores aggregated or dispersed pigment granules when exposed to short- or middle/long-wavelength light, respectively. These results suggest that diverse molecular mechanisms and light-detecting strategies may be employed by different types of tilapia chromatophores, which are instrumental in pigment pattern formation.
format	article
author	Shyh-Chi Chen R Meldrum Robertson Craig W Hawryshyn
author_facet	Shyh-Chi Chen R Meldrum Robertson Craig W Hawryshyn
author_sort	Shyh-Chi Chen
title	Possible involvement of cone opsins in distinct photoresponses of intrinsically photosensitive dermal chromatophores in tilapia Oreochromis niloticus.
title_short	Possible involvement of cone opsins in distinct photoresponses of intrinsically photosensitive dermal chromatophores in tilapia Oreochromis niloticus.
title_full	Possible involvement of cone opsins in distinct photoresponses of intrinsically photosensitive dermal chromatophores in tilapia Oreochromis niloticus.
title_fullStr	Possible involvement of cone opsins in distinct photoresponses of intrinsically photosensitive dermal chromatophores in tilapia Oreochromis niloticus.
title_full_unstemmed	Possible involvement of cone opsins in distinct photoresponses of intrinsically photosensitive dermal chromatophores in tilapia Oreochromis niloticus.
title_sort	possible involvement of cone opsins in distinct photoresponses of intrinsically photosensitive dermal chromatophores in tilapia oreochromis niloticus.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1886790e2f2841f69f13647b8f951eb1
work_keys_str_mv	AT shyhchichen possibleinvolvementofconeopsinsindistinctphotoresponsesofintrinsicallyphotosensitivedermalchromatophoresintilapiaoreochromisniloticus AT rmeldrumrobertson possibleinvolvementofconeopsinsindistinctphotoresponsesofintrinsicallyphotosensitivedermalchromatophoresintilapiaoreochromisniloticus AT craigwhawryshyn possibleinvolvementofconeopsinsindistinctphotoresponsesofintrinsicallyphotosensitivedermalchromatophoresintilapiaoreochromisniloticus
_version_	1718421008729243648

Possible involvement of cone opsins in distinct photoresponses of intrinsically photosensitive dermal chromatophores in tilapia Oreochromis niloticus.

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